Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluC and yceF are separated by 111 nucleotides yceF and yceD are separated by 159 nucleotides yceD and rpmF are separated by 51 nucleotides rpmF and plsX are separated by 110 nucleotides
HEPCGN_23865: rluC - 23S rRNA pseudouridine(955/2504/2580) synthase RluC, at 3,562,952 to 3,563,911
rluC
HEPCGN_23870: yceF - 7-methyl-GTP pyrophosphatase, at 3,564,023 to 3,564,646
yceF
HEPCGN_23880: yceD - 23S rRNA accumulation protein YceD, at 3,564,806 to 3,565,327
yceD
HEPCGN_23885: rpmF - 50S ribosomal protein L32, at 3,565,379 to 3,565,552
rpmF
HEPCGN_23890: plsX - phosphate acyltransferase PlsX, at 3,565,663 to 3,566,703
plsX
Position (kb) 3564
3565
3566 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3563.912 kb on + strand at 3563.913 kb on - strand at 3563.913 kb on - strand at 3563.920 kb on + strand at 3563.923 kb on - strand at 3563.923 kb on - strand at 3563.923 kb on - strand at 3563.951 kb on - strand at 3564.056 kb on + strand at 3564.057 kb on - strand at 3564.057 kb on - strand at 3564.100 kb on + strand, within yceF at 3564.160 kb on + strand, within yceF at 3564.188 kb on - strand, within yceF at 3564.195 kb on + strand, within yceF at 3564.196 kb on - strand, within yceF at 3564.280 kb on + strand, within yceF at 3564.281 kb on - strand, within yceF at 3564.299 kb on - strand, within yceF at 3564.300 kb on + strand, within yceF at 3564.300 kb on + strand, within yceF at 3564.301 kb on - strand, within yceF at 3564.395 kb on + strand, within yceF at 3564.395 kb on + strand, within yceF at 3564.396 kb on - strand, within yceF at 3564.396 kb on - strand, within yceF at 3564.590 kb on + strand at 3564.624 kb on + strand at 3564.626 kb on + strand at 3564.626 kb on + strand at 3564.627 kb on - strand at 3564.632 kb on - strand at 3564.655 kb on - strand at 3564.686 kb on - strand at 3564.775 kb on + strand at 3564.877 kb on + strand, within yceD at 3564.877 kb on + strand, within yceD at 3564.877 kb on + strand, within yceD at 3564.881 kb on + strand, within yceD at 3564.881 kb on + strand, within yceD at 3565.045 kb on + strand, within yceD at 3565.067 kb on + strand, within yceD at 3565.629 kb on + strand at 3565.655 kb on + strand at 3565.775 kb on + strand, within plsX at 3565.782 kb on + strand, within plsX at 3565.783 kb on - strand, within plsX at 3566.263 kb on + strand, within plsX
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 3,563,912 + +0.2 3,563,913 - -0.2 3,563,913 - -0.4 3,563,920 + +0.3 3,563,923 - +0.9 3,563,923 - +0.2 3,563,923 - +0.9 3,563,951 - -1.0 3,564,056 + +1.7 3,564,057 - -0.2 3,564,057 - +0.1 3,564,100 + yceF HEPCGN_23870 0.12 -0.1 3,564,160 + yceF HEPCGN_23870 0.22 -0.3 3,564,188 - yceF HEPCGN_23870 0.26 +1.0 3,564,195 + yceF HEPCGN_23870 0.28 -0.8 3,564,196 - yceF HEPCGN_23870 0.28 -0.2 3,564,280 + yceF HEPCGN_23870 0.41 -0.2 3,564,281 - yceF HEPCGN_23870 0.41 -0.3 3,564,299 - yceF HEPCGN_23870 0.44 -1.0 3,564,300 + yceF HEPCGN_23870 0.44 +0.8 3,564,300 + yceF HEPCGN_23870 0.44 -0.0 3,564,301 - yceF HEPCGN_23870 0.45 +0.2 3,564,395 + yceF HEPCGN_23870 0.60 +1.0 3,564,395 + yceF HEPCGN_23870 0.60 -0.8 3,564,396 - yceF HEPCGN_23870 0.60 -0.1 3,564,396 - yceF HEPCGN_23870 0.60 +0.9 3,564,590 + -0.4 3,564,624 + +0.4 3,564,626 + -0.3 3,564,626 + +1.0 3,564,627 - +1.7 3,564,632 - +0.1 3,564,655 - +1.0 3,564,686 - -0.4 3,564,775 + -2.9 3,564,877 + yceD HEPCGN_23880 0.14 -2.6 3,564,877 + yceD HEPCGN_23880 0.14 +0.3 3,564,877 + yceD HEPCGN_23880 0.14 -3.0 3,564,881 + yceD HEPCGN_23880 0.14 -0.9 3,564,881 + yceD HEPCGN_23880 0.14 -1.4 3,565,045 + yceD HEPCGN_23880 0.46 -1.1 3,565,067 + yceD HEPCGN_23880 0.50 +0.3 3,565,629 + -0.7 3,565,655 + -1.8 3,565,775 + plsX HEPCGN_23890 0.11 -1.5 3,565,782 + plsX HEPCGN_23890 0.11 +0.6 3,565,783 - plsX HEPCGN_23890 0.12 -1.4 3,566,263 + plsX HEPCGN_23890 0.58 +0.7
Or see this region's nucleotide sequence