Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppdA and pulJ overlap by 10 nucleotides pulJ and ygdB overlap by 4 nucleotides ygdB and ppdC overlap by 16 nucleotides ppdC and recC are separated by 12 nucleotides
HEPCGN_19275: ppdA - prepilin peptidase-dependent protein, at 2,606,236 to 2,606,706
ppdA
HEPCGN_19280: pulJ - prepilin peptidase-dependent protein, at 2,606,697 to 2,607,260
pulJ
HEPCGN_19285: ygdB - DUF2509 domain-containing protein, at 2,607,257 to 2,607,664
ygdB
HEPCGN_19290: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,607,649 to 2,607,972
ppdC
HEPCGN_19295: recC - exodeoxyribonuclease V subunit gamma, at 2,607,985 to 2,611,353
recC
Position (kb)
2607
2608 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2606.761 kb on + strand, within pulJ at 2606.761 kb on + strand, within pulJ at 2606.762 kb on - strand, within pulJ at 2606.762 kb on - strand, within pulJ at 2606.797 kb on + strand, within pulJ at 2606.798 kb on - strand, within pulJ at 2606.825 kb on + strand, within pulJ at 2606.895 kb on + strand, within pulJ at 2607.185 kb on + strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.186 kb on - strand, within pulJ at 2607.298 kb on + strand, within ygdB at 2607.325 kb on - strand, within ygdB at 2607.476 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.510 kb on + strand, within ygdB at 2607.532 kb on - strand, within ygdB at 2607.758 kb on + strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.912 kb on - strand, within ppdC at 2607.970 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2607.984 kb on + strand at 2608.101 kb on + strand at 2608.188 kb on + strand at 2608.397 kb on - strand, within recC at 2608.405 kb on + strand, within recC at 2608.509 kb on + strand, within recC at 2608.552 kb on + strand, within recC at 2608.620 kb on + strand, within recC at 2608.620 kb on + strand, within recC at 2608.621 kb on - strand, within recC at 2608.683 kb on + strand, within recC at 2608.737 kb on + strand, within recC at 2608.806 kb on - strand, within recC
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 2,606,761 + pulJ HEPCGN_19280 0.11 +0.1 2,606,761 + pulJ HEPCGN_19280 0.11 -0.5 2,606,762 - pulJ HEPCGN_19280 0.12 +0.3 2,606,762 - pulJ HEPCGN_19280 0.12 -0.1 2,606,797 + pulJ HEPCGN_19280 0.18 -0.1 2,606,798 - pulJ HEPCGN_19280 0.18 -0.8 2,606,825 + pulJ HEPCGN_19280 0.23 +0.2 2,606,895 + pulJ HEPCGN_19280 0.35 -0.2 2,607,185 + pulJ HEPCGN_19280 0.87 +0.3 2,607,186 - pulJ HEPCGN_19280 0.87 +0.1 2,607,186 - pulJ HEPCGN_19280 0.87 -0.0 2,607,298 + ygdB HEPCGN_19285 0.10 -0.8 2,607,325 - ygdB HEPCGN_19285 0.17 +1.0 2,607,476 + ygdB HEPCGN_19285 0.54 +0.1 2,607,510 + ygdB HEPCGN_19285 0.62 +0.2 2,607,510 + ygdB HEPCGN_19285 0.62 +0.8 2,607,532 - ygdB HEPCGN_19285 0.67 -0.5 2,607,758 + ppdC HEPCGN_19290 0.34 -0.2 2,607,912 - ppdC HEPCGN_19290 0.81 -1.0 2,607,912 - ppdC HEPCGN_19290 0.81 -1.1 2,607,970 + +0.8 2,607,984 + +0.4 2,607,984 + -0.5 2,607,984 + -1.3 2,608,101 + -0.0 2,608,188 + -0.0 2,608,397 - recC HEPCGN_19295 0.12 -1.8 2,608,405 + recC HEPCGN_19295 0.12 -0.5 2,608,509 + recC HEPCGN_19295 0.16 +1.8 2,608,552 + recC HEPCGN_19295 0.17 -1.3 2,608,620 + recC HEPCGN_19295 0.19 -1.7 2,608,620 + recC HEPCGN_19295 0.19 +1.2 2,608,621 - recC HEPCGN_19295 0.19 -1.8 2,608,683 + recC HEPCGN_19295 0.21 -3.4 2,608,737 + recC HEPCGN_19295 0.22 -0.9 2,608,806 - recC HEPCGN_19295 0.24 -1.9
Or see this region's nucleotide sequence