Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoA and HEPCGN_16320 are separated by 40 nucleotides HEPCGN_16320 and yhdN are separated by 106 nucleotides yhdN and zntR are separated by 10 nucleotides zntR and arfA are separated by 55 nucleotides arfA and mscL overlap by 4 nucleotides
HEPCGN_16315: rpoA - DNA-directed RNA polymerase subunit alpha, at 2,017,646 to 2,018,635
rpoA
HEPCGN_16320: HEPCGN_16320 - 50S ribosomal protein L17, at 2,018,676 to 2,019,059
_16320
HEPCGN_16325: yhdN - Uncharacterized protein YhdN, at 2,019,166 to 2,019,534
yhdN
HEPCGN_16330: zntR - Zn(2+)-responsive transcriptional regulator, at 2,019,545 to 2,019,970
zntR
HEPCGN_16335: arfA - alternative ribosome-rescue factor ArfA, at 2,020,026 to 2,020,244
arfA
HEPCGN_16340: mscL - large-conductance mechanosensitive channel protein MscL, at 2,020,241 to 2,020,651
mscL
Position (kb)
2019
2020 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2019.108 kb on + strand at 2019.108 kb on + strand at 2019.121 kb on + strand at 2019.121 kb on + strand at 2019.122 kb on - strand at 2019.125 kb on + strand at 2019.130 kb on + strand at 2019.130 kb on + strand at 2019.131 kb on - strand at 2019.136 kb on + strand at 2019.161 kb on + strand at 2019.161 kb on + strand at 2019.161 kb on + strand at 2019.163 kb on + strand at 2019.163 kb on + strand at 2019.163 kb on + strand at 2019.163 kb on + strand at 2019.163 kb on + strand at 2019.164 kb on - strand at 2019.164 kb on - strand at 2019.164 kb on - strand at 2019.164 kb on - strand at 2019.164 kb on - strand at 2019.165 kb on + strand at 2019.165 kb on + strand at 2019.166 kb on - strand at 2019.166 kb on - strand at 2019.166 kb on - strand at 2019.166 kb on - strand at 2019.173 kb on + strand at 2019.198 kb on + strand at 2019.198 kb on + strand at 2019.200 kb on + strand at 2019.200 kb on + strand at 2019.201 kb on - strand at 2019.294 kb on - strand, within yhdN at 2019.441 kb on - strand, within yhdN at 2019.529 kb on - strand at 2019.540 kb on + strand at 2019.540 kb on + strand at 2019.540 kb on + strand at 2019.541 kb on - strand at 2019.544 kb on + strand at 2019.544 kb on + strand at 2019.544 kb on + strand at 2019.544 kb on + strand at 2019.544 kb on + strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.545 kb on - strand at 2019.548 kb on + strand at 2019.548 kb on + strand at 2019.582 kb on + strand at 2019.582 kb on + strand at 2019.637 kb on - strand, within zntR at 2019.637 kb on - strand, within zntR at 2019.663 kb on - strand, within zntR at 2019.663 kb on - strand, within zntR at 2019.850 kb on + strand, within zntR at 2019.901 kb on + strand, within zntR at 2019.912 kb on - strand, within zntR at 2020.005 kb on - strand at 2020.043 kb on + strand at 2020.043 kb on + strand at 2020.044 kb on - strand at 2020.046 kb on + strand at 2020.073 kb on - strand, within arfA at 2020.081 kb on + strand, within arfA at 2020.081 kb on + strand, within arfA at 2020.084 kb on + strand, within arfA at 2020.084 kb on + strand, within arfA at 2020.084 kb on + strand, within arfA at 2020.084 kb on + strand, within arfA at 2020.121 kb on + strand, within arfA at 2020.140 kb on + strand, within arfA at 2020.140 kb on + strand, within arfA at 2020.141 kb on - strand, within arfA at 2020.292 kb on + strand, within mscL at 2020.304 kb on - strand, within mscL at 2020.304 kb on - strand, within mscL at 2020.358 kb on + strand, within mscL at 2020.364 kb on - strand, within mscL at 2020.509 kb on - strand, within mscL at 2020.529 kb on - strand, within mscL
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 2,019,108 + -0.1 2,019,108 + +0.0 2,019,121 + +0.1 2,019,121 + -0.4 2,019,122 - -0.1 2,019,125 + -0.5 2,019,130 + +0.7 2,019,130 + -0.7 2,019,131 - -0.2 2,019,136 + -1.2 2,019,161 + -0.3 2,019,161 + -0.4 2,019,161 + -0.6 2,019,163 + +0.2 2,019,163 + -1.1 2,019,163 + +0.0 2,019,163 + +0.5 2,019,163 + +0.0 2,019,164 - -0.8 2,019,164 - -0.3 2,019,164 - -0.3 2,019,164 - +1.7 2,019,164 - -0.2 2,019,165 + +0.4 2,019,165 + +0.2 2,019,166 - -0.2 2,019,166 - +0.3 2,019,166 - +1.2 2,019,166 - -0.4 2,019,173 + -0.7 2,019,198 + -0.8 2,019,198 + +0.5 2,019,200 + -0.2 2,019,200 + +0.7 2,019,201 - -1.3 2,019,294 - yhdN HEPCGN_16325 0.35 -0.5 2,019,441 - yhdN HEPCGN_16325 0.75 -0.5 2,019,529 - -2.0 2,019,540 + +0.4 2,019,540 + -0.0 2,019,540 + -0.4 2,019,541 - -0.5 2,019,544 + -0.6 2,019,544 + +0.4 2,019,544 + +0.6 2,019,544 + +0.8 2,019,544 + -0.3 2,019,545 - +0.4 2,019,545 - +0.0 2,019,545 - -2.8 2,019,545 - -0.7 2,019,545 - +0.3 2,019,545 - -1.0 2,019,545 - -0.1 2,019,548 + +0.2 2,019,548 + +0.7 2,019,582 + +0.2 2,019,582 + +0.8 2,019,637 - zntR HEPCGN_16330 0.22 -1.6 2,019,637 - zntR HEPCGN_16330 0.22 -0.6 2,019,663 - zntR HEPCGN_16330 0.28 -0.2 2,019,663 - zntR HEPCGN_16330 0.28 -0.5 2,019,850 + zntR HEPCGN_16330 0.72 -0.1 2,019,901 + zntR HEPCGN_16330 0.84 -0.1 2,019,912 - zntR HEPCGN_16330 0.86 -0.3 2,020,005 - +1.0 2,020,043 + +0.2 2,020,043 + -0.1 2,020,044 - -0.7 2,020,046 + +0.6 2,020,073 - arfA HEPCGN_16335 0.21 -0.5 2,020,081 + arfA HEPCGN_16335 0.25 +1.0 2,020,081 + arfA HEPCGN_16335 0.25 -0.2 2,020,084 + arfA HEPCGN_16335 0.26 -0.0 2,020,084 + arfA HEPCGN_16335 0.26 -0.4 2,020,084 + arfA HEPCGN_16335 0.26 -0.3 2,020,084 + arfA HEPCGN_16335 0.26 -1.2 2,020,121 + arfA HEPCGN_16335 0.43 +0.2 2,020,140 + arfA HEPCGN_16335 0.52 -2.5 2,020,140 + arfA HEPCGN_16335 0.52 -0.4 2,020,141 - arfA HEPCGN_16335 0.53 -2.3 2,020,292 + mscL HEPCGN_16340 0.12 -1.3 2,020,304 - mscL HEPCGN_16340 0.15 -0.5 2,020,304 - mscL HEPCGN_16340 0.15 -0.3 2,020,358 + mscL HEPCGN_16340 0.28 -1.2 2,020,364 - mscL HEPCGN_16340 0.30 -0.1 2,020,509 - mscL HEPCGN_16340 0.65 +1.1 2,020,529 - mscL HEPCGN_16340 0.70 +0.5
Or see this region's nucleotide sequence