Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmA and apaG are separated by 2 nucleotides apaG and apaH are separated by 6 nucleotides apaH and HEPCGN_08840 are separated by 185 nucleotides HEPCGN_08840 and HEPCGN_08845 are separated by 2 nucleotides HEPCGN_08845 and HEPCGN_08850 are separated by 85 nucleotides
HEPCGN_08825: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 509,986 to 510,807
rsmA
HEPCGN_08830: apaG - Co2+/Mg2+ efflux protein ApaG, at 510,810 to 511,187
apaG
HEPCGN_08835: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 511,194 to 512,042
apaH
HEPCGN_08840: HEPCGN_08840 - Toxin CcdB, at 512,228 to 512,353
_08840
HEPCGN_08845: HEPCGN_08845 - antitoxin, at 512,356 to 512,589
_08845
HEPCGN_08850: HEPCGN_08850 - Dihydrofolate reductase, at 512,675 to 513,265
_08850
Position (kb) 511
512
513 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 510.332 kb on - strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.334 kb on - strand, within rsmA at 510.334 kb on - strand, within rsmA at 510.367 kb on - strand, within rsmA at 510.371 kb on - strand, within rsmA at 510.371 kb on - strand, within rsmA at 510.399 kb on + strand, within rsmA at 510.399 kb on + strand, within rsmA at 510.454 kb on + strand, within rsmA at 510.481 kb on + strand, within rsmA at 510.663 kb on + strand, within rsmA at 510.773 kb on - strand at 510.853 kb on - strand, within apaG at 510.901 kb on - strand, within apaG at 510.913 kb on + strand, within apaG at 511.028 kb on + strand, within apaG at 511.053 kb on + strand, within apaG at 511.185 kb on + strand at 511.203 kb on + strand at 511.203 kb on + strand at 511.203 kb on + strand at 511.204 kb on - strand at 511.204 kb on - strand at 511.204 kb on - strand at 511.208 kb on + strand at 511.230 kb on + strand at 511.231 kb on - strand at 511.256 kb on + strand at 511.261 kb on + strand at 511.269 kb on - strand at 511.283 kb on + strand, within apaH at 511.369 kb on + strand, within apaH at 511.370 kb on - strand, within apaH at 511.370 kb on - strand, within apaH at 511.434 kb on - strand, within apaH at 511.491 kb on - strand, within apaH at 511.609 kb on + strand, within apaH at 511.610 kb on - strand, within apaH at 511.610 kb on - strand, within apaH at 511.635 kb on + strand, within apaH at 511.659 kb on + strand, within apaH at 511.660 kb on - strand, within apaH at 511.772 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.946 kb on + strand, within apaH at 511.947 kb on - strand, within apaH at 511.973 kb on + strand at 511.974 kb on - strand at 511.981 kb on - strand at 512.164 kb on - strand at 512.164 kb on - strand at 512.178 kb on - strand at 512.178 kb on - strand at 512.183 kb on + strand at 512.183 kb on + strand at 512.237 kb on - strand at 512.291 kb on - strand, within HEPCGN_08840 at 512.291 kb on - strand, within HEPCGN_08840 at 512.340 kb on - strand, within HEPCGN_08840 at 512.506 kb on - strand, within HEPCGN_08845 at 512.509 kb on + strand, within HEPCGN_08845 at 512.575 kb on - strand at 512.613 kb on - strand at 512.620 kb on + strand at 512.620 kb on + strand at 512.620 kb on + strand at 512.621 kb on - strand at 512.623 kb on + strand at 512.623 kb on + strand at 512.624 kb on - strand at 512.624 kb on - strand at 512.625 kb on + strand at 512.633 kb on + strand at 512.643 kb on + strand at 512.643 kb on + strand at 512.644 kb on - strand at 512.644 kb on - strand at 512.647 kb on - strand at 512.647 kb on - strand at 512.664 kb on - strand at 512.668 kb on + strand at 512.668 kb on + strand at 512.669 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 510,332 - rsmA HEPCGN_08825 0.42 -0.8 510,333 + rsmA HEPCGN_08825 0.42 -2.1 510,333 + rsmA HEPCGN_08825 0.42 -0.6 510,333 + rsmA HEPCGN_08825 0.42 -0.0 510,333 + rsmA HEPCGN_08825 0.42 +2.5 510,334 - rsmA HEPCGN_08825 0.42 +0.3 510,334 - rsmA HEPCGN_08825 0.42 -0.3 510,367 - rsmA HEPCGN_08825 0.46 -0.8 510,371 - rsmA HEPCGN_08825 0.47 -0.5 510,371 - rsmA HEPCGN_08825 0.47 -1.3 510,399 + rsmA HEPCGN_08825 0.50 -1.1 510,399 + rsmA HEPCGN_08825 0.50 +0.0 510,454 + rsmA HEPCGN_08825 0.57 -2.3 510,481 + rsmA HEPCGN_08825 0.60 +0.0 510,663 + rsmA HEPCGN_08825 0.82 -0.7 510,773 - -1.0 510,853 - apaG HEPCGN_08830 0.11 +0.8 510,901 - apaG HEPCGN_08830 0.24 +0.2 510,913 + apaG HEPCGN_08830 0.27 -0.7 511,028 + apaG HEPCGN_08830 0.58 -0.6 511,053 + apaG HEPCGN_08830 0.64 +0.2 511,185 + -0.1 511,203 + -2.5 511,203 + -0.0 511,203 + -1.9 511,204 - -0.1 511,204 - -2.0 511,204 - -3.0 511,208 + -3.1 511,230 + -0.1 511,231 - -0.1 511,256 + -3.2 511,261 + -1.9 511,269 - +0.8 511,283 + apaH HEPCGN_08835 0.10 -1.9 511,369 + apaH HEPCGN_08835 0.21 -1.9 511,370 - apaH HEPCGN_08835 0.21 -2.2 511,370 - apaH HEPCGN_08835 0.21 -0.8 511,434 - apaH HEPCGN_08835 0.28 -1.8 511,491 - apaH HEPCGN_08835 0.35 -0.7 511,609 + apaH HEPCGN_08835 0.49 +0.1 511,610 - apaH HEPCGN_08835 0.49 -1.2 511,610 - apaH HEPCGN_08835 0.49 -2.5 511,635 + apaH HEPCGN_08835 0.52 -2.3 511,659 + apaH HEPCGN_08835 0.55 -0.6 511,660 - apaH HEPCGN_08835 0.55 -0.7 511,772 + apaH HEPCGN_08835 0.68 +0.3 511,776 + apaH HEPCGN_08835 0.69 +0.1 511,776 + apaH HEPCGN_08835 0.69 -2.0 511,946 + apaH HEPCGN_08835 0.89 +1.9 511,947 - apaH HEPCGN_08835 0.89 -1.4 511,973 + -0.2 511,974 - +0.3 511,981 - -0.8 512,164 - +0.0 512,164 - -0.8 512,178 - -0.4 512,178 - +0.1 512,183 + +0.5 512,183 + +0.2 512,237 - +0.1 512,291 - HEPCGN_08840 0.50 +0.6 512,291 - HEPCGN_08840 0.50 +1.9 512,340 - HEPCGN_08840 0.89 -0.3 512,506 - HEPCGN_08845 0.64 +0.1 512,509 + HEPCGN_08845 0.65 +0.8 512,575 - -0.0 512,613 - +0.7 512,620 + +0.2 512,620 + -1.1 512,620 + +0.2 512,621 - +0.1 512,623 + +1.4 512,623 + -1.4 512,624 - -0.5 512,624 - +0.1 512,625 + +0.6 512,633 + -0.4 512,643 + -1.2 512,643 + +0.3 512,644 - -0.3 512,644 - -0.3 512,647 - +0.1 512,647 - -0.1 512,664 - -0.0 512,668 + -1.3 512,668 + +0.4 512,669 - -1.6
Or see this region's nucleotide sequence