Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ribD and nrdR are separated by 3 nucleotides nrdR and HEPCGN_07090 are separated by 150 nucleotides HEPCGN_07090 and HEPCGN_07095 overlap by 16 nucleotides
HEPCGN_07080: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 150,828 to 151,931
ribD
HEPCGN_07085: nrdR - transcriptional regulator NrdR, at 151,935 to 152,384
nrdR
HEPCGN_07090: HEPCGN_07090 - hypothetical protein, at 152,535 to 152,723
_07090
HEPCGN_07095: HEPCGN_07095 - RNA-guided endonuclease TnpB family protein, at 152,708 to 153,514
_07095
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-2
-1
0
1 at 151.977 kb on - strand at 152.015 kb on - strand, within nrdR at 152.025 kb on - strand, within nrdR at 152.188 kb on - strand, within nrdR at 152.283 kb on - strand, within nrdR at 152.283 kb on - strand, within nrdR at 152.352 kb on - strand at 152.438 kb on + strand at 152.439 kb on - strand at 152.466 kb on - strand at 152.491 kb on + strand at 152.534 kb on + strand at 152.535 kb on - strand at 152.535 kb on - strand at 152.540 kb on + strand at 152.540 kb on + strand at 152.540 kb on + strand at 152.541 kb on - strand at 152.541 kb on - strand at 152.543 kb on + strand at 152.843 kb on - strand, within HEPCGN_07095 at 152.852 kb on + strand, within HEPCGN_07095 at 152.852 kb on + strand, within HEPCGN_07095 at 152.932 kb on - strand, within HEPCGN_07095 at 152.932 kb on - strand, within HEPCGN_07095
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 151,977 - -2.4 152,015 - nrdR HEPCGN_07085 0.18 -0.8 152,025 - nrdR HEPCGN_07085 0.20 -0.6 152,188 - nrdR HEPCGN_07085 0.56 -2.3 152,283 - nrdR HEPCGN_07085 0.77 -0.1 152,283 - nrdR HEPCGN_07085 0.77 -0.5 152,352 - -1.2 152,438 + +0.7 152,439 - -0.2 152,466 - +1.1 152,491 + -1.9 152,534 + +0.7 152,535 - -0.5 152,535 - -0.0 152,540 + +0.6 152,540 + -0.3 152,540 + -0.1 152,541 - +0.9 152,541 - +0.2 152,543 + -0.6 152,843 - HEPCGN_07095 0.17 -0.3 152,852 + HEPCGN_07095 0.18 +0.2 152,852 + HEPCGN_07095 0.18 -0.9 152,932 - HEPCGN_07095 0.28 +0.2 152,932 - HEPCGN_07095 0.28 -0.1
Or see this region's nucleotide sequence