Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and HEPCGN_04680 are separated by 1 nucleotides HEPCGN_04680 and yobI are separated by 70 nucleotides yobI and ruvA are separated by 138 nucleotides ruvA and ruvB are separated by 8 nucleotides
HEPCGN_04675: ruvC - crossover junction endodeoxyribonuclease RuvC, at 815,432 to 815,953
ruvC
HEPCGN_04680: HEPCGN_04680 - Protein, at 815,955 to 816,557
_04680
HEPCGN_04685: yobI - Uncharacterized protein YobI, at 816,628 to 816,693
yobI
HEPCGN_04690: ruvA - Holliday junction branch migration protein RuvA, at 816,832 to 817,443
ruvA
HEPCGN_04695: ruvB - Holliday junction branch migration DNA helicase RuvB, at 817,452 to 818,462
ruvB
Position (kb) 816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 815.766 kb on + strand, within ruvC at 815.862 kb on + strand, within ruvC at 815.862 kb on + strand, within ruvC at 815.867 kb on - strand, within ruvC at 815.952 kb on - strand at 815.958 kb on + strand at 816.302 kb on + strand, within HEPCGN_04680 at 816.324 kb on + strand, within HEPCGN_04680 at 816.332 kb on - strand, within HEPCGN_04680 at 816.425 kb on + strand, within HEPCGN_04680 at 816.503 kb on + strand at 816.503 kb on + strand at 816.504 kb on - strand at 816.504 kb on - strand at 816.639 kb on + strand, within yobI at 817.542 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 815,766 + ruvC HEPCGN_04675 0.64 -1.0 815,862 + ruvC HEPCGN_04675 0.82 -0.5 815,862 + ruvC HEPCGN_04675 0.82 -1.4 815,867 - ruvC HEPCGN_04675 0.83 +0.1 815,952 - -0.6 815,958 + -0.2 816,302 + HEPCGN_04680 0.58 +0.4 816,324 + HEPCGN_04680 0.61 -0.8 816,332 - HEPCGN_04680 0.63 -0.2 816,425 + HEPCGN_04680 0.78 +1.4 816,503 + +0.6 816,503 + -2.4 816,504 - +0.0 816,504 - -0.8 816,639 + yobI HEPCGN_04685 0.17 -3.0 817,542 + +0.1
Or see this region's nucleotide sequence