Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisG are separated by 5 nucleotides hisG and hisL are separated by 145 nucleotides hisL and yeeZ are separated by 426 nucleotides
HEPCGN_03220: hisD - Histidinol dehydrogenase, at 555,681 to 556,985
hisD
HEPCGN_03225: hisG - ATP phosphoribosyltransferase, at 556,991 to 557,890
hisG
HEPCGN_03230: hisL - his operon leader peptide, at 558,036 to 558,086
hisL
HEPCGN_03235: yeeZ - Protein YeeZ, at 558,513 to 559,337
yeeZ
Position (kb) 556
557
558 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 556.292 kb on + strand, within hisD at 556.323 kb on + strand, within hisD at 556.598 kb on - strand, within hisD at 556.598 kb on - strand, within hisD at 556.598 kb on - strand, within hisD at 556.615 kb on - strand, within hisD at 556.641 kb on + strand, within hisD at 556.659 kb on + strand, within hisD at 556.659 kb on + strand, within hisD at 556.704 kb on - strand, within hisD at 556.734 kb on + strand, within hisD at 556.735 kb on - strand, within hisD at 556.825 kb on + strand, within hisD at 556.955 kb on + strand at 556.955 kb on + strand at 556.956 kb on - strand at 556.976 kb on + strand at 557.102 kb on + strand, within hisG at 557.134 kb on + strand, within hisG at 557.208 kb on + strand, within hisG at 557.329 kb on + strand, within hisG at 557.329 kb on + strand, within hisG at 557.330 kb on - strand, within hisG at 557.353 kb on + strand, within hisG at 557.442 kb on - strand, within hisG at 557.499 kb on + strand, within hisG at 557.512 kb on + strand, within hisG at 557.800 kb on + strand, within hisG at 557.865 kb on - strand at 557.896 kb on + strand at 557.897 kb on - strand at 558.164 kb on + strand at 558.164 kb on + strand at 558.229 kb on + strand at 558.230 kb on - strand at 558.230 kb on - strand at 558.255 kb on - strand at 558.294 kb on - strand at 558.368 kb on - strand at 558.368 kb on - strand at 558.415 kb on - strand at 558.417 kb on - strand at 558.471 kb on + strand at 558.482 kb on + strand at 558.484 kb on + strand at 558.485 kb on - strand at 558.485 kb on - strand at 558.485 kb on - strand at 558.539 kb on - strand at 558.651 kb on - strand, within yeeZ at 558.654 kb on + strand, within yeeZ at 558.680 kb on - strand, within yeeZ at 558.779 kb on - strand, within yeeZ
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 556,292 + hisD HEPCGN_03220 0.47 -1.0 556,323 + hisD HEPCGN_03220 0.49 -0.2 556,598 - hisD HEPCGN_03220 0.70 +1.2 556,598 - hisD HEPCGN_03220 0.70 -1.9 556,598 - hisD HEPCGN_03220 0.70 -0.3 556,615 - hisD HEPCGN_03220 0.72 +0.2 556,641 + hisD HEPCGN_03220 0.74 -0.5 556,659 + hisD HEPCGN_03220 0.75 +0.9 556,659 + hisD HEPCGN_03220 0.75 -1.1 556,704 - hisD HEPCGN_03220 0.78 +0.4 556,734 + hisD HEPCGN_03220 0.81 +0.9 556,735 - hisD HEPCGN_03220 0.81 +0.6 556,825 + hisD HEPCGN_03220 0.88 +0.7 556,955 + -0.3 556,955 + +0.5 556,956 - -1.1 556,976 + -0.3 557,102 + hisG HEPCGN_03225 0.12 +0.3 557,134 + hisG HEPCGN_03225 0.16 +0.4 557,208 + hisG HEPCGN_03225 0.24 -0.3 557,329 + hisG HEPCGN_03225 0.38 +0.3 557,329 + hisG HEPCGN_03225 0.38 +0.1 557,330 - hisG HEPCGN_03225 0.38 +1.0 557,353 + hisG HEPCGN_03225 0.40 +0.6 557,442 - hisG HEPCGN_03225 0.50 -1.8 557,499 + hisG HEPCGN_03225 0.56 +1.0 557,512 + hisG HEPCGN_03225 0.58 +2.8 557,800 + hisG HEPCGN_03225 0.90 +1.6 557,865 - +0.4 557,896 + +0.7 557,897 - -0.4 558,164 + -0.3 558,164 + -0.5 558,229 + +0.5 558,230 - +0.5 558,230 - -2.1 558,255 - +0.5 558,294 - -0.1 558,368 - +0.7 558,368 - -0.7 558,415 - -0.4 558,417 - +0.7 558,471 + -0.3 558,482 + -2.7 558,484 + -0.0 558,485 - -0.4 558,485 - -0.8 558,485 - -0.5 558,539 - +0.1 558,651 - yeeZ HEPCGN_03235 0.17 +0.4 558,654 + yeeZ HEPCGN_03235 0.17 -0.6 558,680 - yeeZ HEPCGN_03235 0.20 +2.0 558,779 - yeeZ HEPCGN_03235 0.32 +1.4
Or see this region's nucleotide sequence