Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03060

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfcl and gmm overlap by 1 nucleotidesgmm and wcaI overlap by 4 nucleotideswcaI and manC are separated by 2 nucleotides HEPCGN_03050: fcl - GDP-L-fucose synthase, at 518,647 to 519,612 fcl HEPCGN_03055: gmm - GDP-mannose mannosyl hydrolase, at 519,612 to 520,094 gmm HEPCGN_03060: wcaI - colanic acid biosynthesis fucosyltransferase WcaI, at 520,091 to 521,314 wcaI HEPCGN_03065: manC - mannose-1-phosphate guanylyltransferase ManC, at 521,317 to 522,753 manC Position (kb) 520 521 522Strain fitness (log2 ratio) -1 0 1at 519.174 kb on + strand, within fclat 519.483 kb on + strand, within fclat 519.571 kb on + strandat 519.610 kb on + strandat 519.647 kb on - strandat 519.847 kb on + strand, within gmmat 519.848 kb on - strand, within gmmat 519.848 kb on - strand, within gmmat 519.887 kb on + strand, within gmmat 519.888 kb on - strand, within gmmat 519.919 kb on - strand, within gmmat 519.919 kb on - strand, within gmmat 520.098 kb on + strandat 520.148 kb on + strandat 520.148 kb on + strandat 520.149 kb on - strandat 520.201 kb on + strandat 520.247 kb on + strand, within wcaIat 520.594 kb on + strand, within wcaIat 520.648 kb on + strand, within wcaIat 520.649 kb on - strand, within wcaIat 520.797 kb on - strand, within wcaIat 520.807 kb on + strand, within wcaIat 520.837 kb on + strand, within wcaIat 521.050 kb on - strand, within wcaIat 521.249 kb on + strandat 521.295 kb on + strandat 521.302 kb on + strandat 521.303 kb on - strandat 521.316 kb on + strandat 521.317 kb on - strandat 521.563 kb on - strand, within manCat 521.632 kb on - strand, within manCat 521.651 kb on + strand, within manCat 521.652 kb on - strand, within manCat 521.652 kb on - strand, within manCat 521.728 kb on + strand, within manCat 521.729 kb on - strand, within manCat 521.795 kb on - strand, within manCat 521.893 kb on + strand, within manCat 521.894 kb on - strand, within manCat 521.953 kb on + strand, within manCat 521.953 kb on + strand, within manCat 522.250 kb on + strand, within manCat 522.250 kb on + strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.251 kb on - strand, within manCat 522.257 kb on - strand, within manC

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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519,174 + fcl HEPCGN_03050 0.55 +0.4
519,483 + fcl HEPCGN_03050 0.87 +0.3
519,571 + -1.0
519,610 + -0.6
519,647 - -1.0
519,847 + gmm HEPCGN_03055 0.49 -0.0
519,848 - gmm HEPCGN_03055 0.49 -0.6
519,848 - gmm HEPCGN_03055 0.49 -0.7
519,887 + gmm HEPCGN_03055 0.57 -0.0
519,888 - gmm HEPCGN_03055 0.57 -0.5
519,919 - gmm HEPCGN_03055 0.64 -0.3
519,919 - gmm HEPCGN_03055 0.64 -0.1
520,098 + +1.2
520,148 + -0.1
520,148 + +0.3
520,149 - -0.3
520,201 + +0.3
520,247 + wcaI HEPCGN_03060 0.13 +0.5
520,594 + wcaI HEPCGN_03060 0.41 +1.0
520,648 + wcaI HEPCGN_03060 0.46 +1.4
520,649 - wcaI HEPCGN_03060 0.46 -0.0
520,797 - wcaI HEPCGN_03060 0.58 +0.2
520,807 + wcaI HEPCGN_03060 0.58 +0.9
520,837 + wcaI HEPCGN_03060 0.61 -0.7
521,050 - wcaI HEPCGN_03060 0.78 +1.3
521,249 + -0.5
521,295 + +0.9
521,302 + -0.6
521,303 - +0.6
521,316 + +0.5
521,317 - +1.5
521,563 - manC HEPCGN_03065 0.17 -0.5
521,632 - manC HEPCGN_03065 0.22 +1.2
521,651 + manC HEPCGN_03065 0.23 -0.4
521,652 - manC HEPCGN_03065 0.23 +0.1
521,652 - manC HEPCGN_03065 0.23 +0.2
521,728 + manC HEPCGN_03065 0.29 +0.1
521,729 - manC HEPCGN_03065 0.29 -1.4
521,795 - manC HEPCGN_03065 0.33 -0.1
521,893 + manC HEPCGN_03065 0.40 -0.1
521,894 - manC HEPCGN_03065 0.40 -0.2
521,953 + manC HEPCGN_03065 0.44 -0.2
521,953 + manC HEPCGN_03065 0.44 +0.4
522,250 + manC HEPCGN_03065 0.65 -1.1
522,250 + manC HEPCGN_03065 0.65 +0.1
522,251 - manC HEPCGN_03065 0.65 +0.3
522,251 - manC HEPCGN_03065 0.65 +0.5
522,251 - manC HEPCGN_03065 0.65 +0.2
522,251 - manC HEPCGN_03065 0.65 +1.6
522,251 - manC HEPCGN_03065 0.65 -0.1
522,251 - manC HEPCGN_03065 0.65 +0.7
522,251 - manC HEPCGN_03065 0.65 -0.1
522,251 - manC HEPCGN_03065 0.65 +1.1
522,257 - manC HEPCGN_03065 0.65 +0.4

Or see this region's nucleotide sequence