Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and cydX are separated by 14 nucleotides cydX and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides
HEPCGN_01430: cydB - cytochrome d ubiquinol oxidase subunit II, at 217,918 to 219,057
cydB
HEPCGN_01435: cydX - cytochrome bd-I oxidase subunit CydX, at 219,072 to 219,185
cydX
HEPCGN_01440: ybgE - cyd operon protein YbgE, at 219,185 to 219,478
ybgE
HEPCGN_01445: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 219,628 to 220,032
ybgC
HEPCGN_01450: tolQ - Tol-Pal system protein TolQ, at 220,029 to 220,721
tolQ
HEPCGN_01455: tolR - colicin uptake protein TolR, at 220,725 to 221,153
tolR
HEPCGN_01460: tolA - cell envelope integrity protein TolA, at 221,218 to 222,405
tolA
Position (kb) 220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 219.055 kb on + strand at 219.059 kb on + strand at 219.196 kb on + strand at 219.210 kb on - strand at 219.522 kb on - strand at 219.575 kb on + strand at 219.576 kb on - strand at 219.581 kb on - strand at 219.581 kb on - strand at 219.581 kb on - strand at 219.591 kb on - strand at 219.624 kb on + strand at 219.624 kb on + strand at 219.624 kb on + strand at 219.633 kb on + strand at 219.634 kb on - strand at 219.664 kb on + strand at 219.667 kb on + strand at 219.667 kb on + strand at 219.667 kb on + strand at 219.668 kb on - strand at 219.676 kb on - strand, within ybgC at 219.709 kb on + strand, within ybgC at 219.804 kb on - strand, within ybgC at 219.808 kb on - strand, within ybgC at 219.827 kb on - strand, within ybgC at 219.872 kb on + strand, within ybgC at 219.912 kb on + strand, within ybgC at 219.913 kb on - strand, within ybgC at 219.930 kb on + strand, within ybgC at 220.009 kb on - strand at 220.043 kb on + strand at 220.044 kb on - strand at 220.224 kb on + strand, within tolQ at 220.367 kb on + strand, within tolQ at 220.368 kb on - strand, within tolQ at 220.368 kb on - strand, within tolQ at 220.443 kb on + strand, within tolQ at 220.603 kb on - strand, within tolQ at 220.603 kb on - strand, within tolQ at 220.604 kb on + strand, within tolQ at 220.604 kb on + strand, within tolQ at 220.780 kb on + strand, within tolR at 220.796 kb on - strand, within tolR at 220.944 kb on + strand, within tolR at 220.944 kb on + strand, within tolR at 220.945 kb on - strand, within tolR at 220.945 kb on - strand, within tolR at 221.074 kb on - strand, within tolR at 221.087 kb on + strand, within tolR at 221.104 kb on + strand, within tolR at 221.151 kb on + strand at 221.151 kb on + strand at 221.291 kb on + strand at 221.330 kb on + strand at 221.397 kb on - strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 219,055 + +0.9 219,059 + +0.6 219,196 + +0.9 219,210 - -1.0 219,522 - +0.2 219,575 + +0.7 219,576 - +0.9 219,581 - -0.5 219,581 - -0.2 219,581 - -0.4 219,591 - -0.5 219,624 + -0.1 219,624 + +0.3 219,624 + +0.1 219,633 + -0.1 219,634 - -0.5 219,664 + -1.4 219,667 + -0.4 219,667 + +0.2 219,667 + +0.2 219,668 - -0.9 219,676 - ybgC HEPCGN_01445 0.12 -2.0 219,709 + ybgC HEPCGN_01445 0.20 -0.7 219,804 - ybgC HEPCGN_01445 0.43 -1.1 219,808 - ybgC HEPCGN_01445 0.44 -1.7 219,827 - ybgC HEPCGN_01445 0.49 -0.3 219,872 + ybgC HEPCGN_01445 0.60 -0.8 219,912 + ybgC HEPCGN_01445 0.70 -1.4 219,913 - ybgC HEPCGN_01445 0.70 -1.9 219,930 + ybgC HEPCGN_01445 0.75 -0.1 220,009 - -1.3 220,043 + -2.4 220,044 - -1.4 220,224 + tolQ HEPCGN_01450 0.28 -1.9 220,367 + tolQ HEPCGN_01450 0.49 -0.7 220,368 - tolQ HEPCGN_01450 0.49 +0.3 220,368 - tolQ HEPCGN_01450 0.49 -1.1 220,443 + tolQ HEPCGN_01450 0.60 -0.3 220,603 - tolQ HEPCGN_01450 0.83 -1.4 220,603 - tolQ HEPCGN_01450 0.83 -1.6 220,604 + tolQ HEPCGN_01450 0.83 -1.4 220,604 + tolQ HEPCGN_01450 0.83 -1.6 220,780 + tolR HEPCGN_01455 0.13 -0.9 220,796 - tolR HEPCGN_01455 0.17 -0.1 220,944 + tolR HEPCGN_01455 0.51 +1.6 220,944 + tolR HEPCGN_01455 0.51 -2.0 220,945 - tolR HEPCGN_01455 0.51 -0.4 220,945 - tolR HEPCGN_01455 0.51 -0.4 221,074 - tolR HEPCGN_01455 0.81 +1.6 221,087 + tolR HEPCGN_01455 0.84 -2.9 221,104 + tolR HEPCGN_01455 0.88 +0.6 221,151 + -0.1 221,151 + -0.4 221,291 + -1.0 221,330 + -0.5 221,397 - tolA HEPCGN_01460 0.15 -3.0
Or see this region's nucleotide sequence