Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt chiP and chiQ are separated by 49 nucleotides chiQ and fur are separated by 83 nucleotides fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides fldA and ybfE are separated by 139 nucleotides
HEPCGN_01085: chiP - chitoporin, at 142,912 to 144,318
chiP
HEPCGN_01090: chiQ - Uncharacterized lipoprotein ChiQ, at 144,368 to 144,694
chiQ
HEPCGN_01095: fur - ferric iron uptake transcriptional regulator, at 144,778 to 145,224
fur
HEPCGN_01100: uof - fur leader peptide, at 145,217 to 145,303
uof
HEPCGN_01105: fldA - flavodoxin FldA, at 145,513 to 146,043
fldA
HEPCGN_01110: ybfE - LexA regulated protein, at 146,183 to 146,545
ybfE
Position (kb) 145
146 Strain fitness (log2 ratio) -2
-1
0
1 at 144.294 kb on - strand at 144.411 kb on + strand, within chiQ at 144.464 kb on + strand, within chiQ at 144.465 kb on - strand, within chiQ at 144.475 kb on + strand, within chiQ at 144.597 kb on - strand, within chiQ at 144.682 kb on + strand at 144.693 kb on - strand at 144.693 kb on - strand at 144.706 kb on - strand at 144.779 kb on + strand at 145.061 kb on - strand, within fur at 145.107 kb on - strand, within fur at 145.242 kb on - strand, within uof at 145.307 kb on - strand at 145.358 kb on + strand at 145.377 kb on - strand at 145.377 kb on - strand at 145.382 kb on + strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.383 kb on - strand at 145.488 kb on - strand at 146.068 kb on - strand at 146.166 kb on + strand at 146.226 kb on - strand, within ybfE at 146.257 kb on + strand, within ybfE at 146.257 kb on + strand, within ybfE at 146.257 kb on + strand, within ybfE
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 144,294 - +0.4 144,411 + chiQ HEPCGN_01090 0.13 +0.5 144,464 + chiQ HEPCGN_01090 0.29 +0.8 144,465 - chiQ HEPCGN_01090 0.30 -1.2 144,475 + chiQ HEPCGN_01090 0.33 +0.0 144,597 - chiQ HEPCGN_01090 0.70 +0.2 144,682 + +0.1 144,693 - -0.4 144,693 - -0.4 144,706 - +1.1 144,779 + +1.5 145,061 - fur HEPCGN_01095 0.63 +1.0 145,107 - fur HEPCGN_01095 0.74 +0.6 145,242 - uof HEPCGN_01100 0.29 -2.2 145,307 - +0.7 145,358 + +0.3 145,377 - -2.3 145,377 - +0.0 145,382 + -1.5 145,383 - -0.4 145,383 - -0.0 145,383 - -0.4 145,488 - +0.7 146,068 - -0.5 146,166 + -0.5 146,226 - ybfE HEPCGN_01110 0.12 +0.1 146,257 + ybfE HEPCGN_01110 0.20 -0.0 146,257 + ybfE HEPCGN_01110 0.20 -0.1 146,257 + ybfE HEPCGN_01110 0.20 +0.0
Or see this region's nucleotide sequence