Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00225 and HEPCGN_00230 overlap by 4 nucleotides HEPCGN_00230 and HEPCGN_00235 overlap by 14 nucleotides HEPCGN_00235 and HEPCGN_00240 are separated by 453 nucleotides HEPCGN_00240 and HEPCGN_00245 are separated by 51 nucleotides HEPCGN_00245 and HEPCGN_00250 overlap by 4 nucleotides HEPCGN_00250 and HEPCGN_00255 are separated by 82 nucleotides
HEPCGN_00225: HEPCGN_00225 - hypothetical protein, at 14,273 to 14,815
_00225
HEPCGN_00230: HEPCGN_00230 - hypothetical protein, at 14,812 to 15,063
_00230
HEPCGN_00235: HEPCGN_00235 - XRE family transcriptional regulator, at 15,050 to 15,277
_00235
HEPCGN_00240: HEPCGN_00240 - hypothetical protein, at 15,731 to 16,030
_00240
HEPCGN_00245: HEPCGN_00245 - Phage tail protein, at 16,082 to 16,456
_00245
HEPCGN_00250: HEPCGN_00250 - hypothetical protein, at 16,453 to 16,764
_00250
HEPCGN_00255: HEPCGN_00255 - hypothetical protein, at 16,847 to 16,984
_00255
Position (kb) 15
16
17 Strain fitness (log2 ratio) -2
-1
0
1
2 at 14.773 kb on - strand at 14.938 kb on + strand, within HEPCGN_00230 at 14.939 kb on - strand, within HEPCGN_00230 at 15.296 kb on + strand at 15.296 kb on + strand at 15.297 kb on - strand at 15.297 kb on - strand at 15.297 kb on - strand at 15.301 kb on + strand at 15.301 kb on + strand at 15.302 kb on - strand at 15.323 kb on - strand at 15.705 kb on + strand at 15.705 kb on + strand at 15.806 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 15.837 kb on - strand, within HEPCGN_00240 at 16.136 kb on - strand, within HEPCGN_00245 at 16.529 kb on + strand, within HEPCGN_00250 at 16.730 kb on + strand, within HEPCGN_00250 at 16.970 kb on + strand, within HEPCGN_00255
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 14,773 - +1.8 14,938 + HEPCGN_00230 0.50 -0.4 14,939 - HEPCGN_00230 0.50 -2.1 15,296 + -0.6 15,296 + -0.4 15,297 - +0.6 15,297 - +0.2 15,297 - +0.5 15,301 + +0.0 15,301 + +0.3 15,302 - +0.7 15,323 - -0.1 15,705 + +0.5 15,705 + -1.9 15,806 - HEPCGN_00240 0.25 +0.4 15,837 - HEPCGN_00240 0.35 +0.5 15,837 - HEPCGN_00240 0.35 +1.2 15,837 - HEPCGN_00240 0.35 +0.0 16,136 - HEPCGN_00245 0.14 -0.2 16,529 + HEPCGN_00250 0.24 -0.2 16,730 + HEPCGN_00250 0.89 -0.2 16,970 + HEPCGN_00255 0.89 +1.3
Or see this region's nucleotide sequence