Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_00045 and iss are separated by 168 nucleotides iss and rzpD are separated by 31 nucleotides rzpD and rrrD overlap by 4 nucleotides rrrD and essD overlap by 1 nucleotides essD and HEPCGN_00070 are separated by 188 nucleotides
HEPCGN_00045: HEPCGN_00045 - Arginyl-tRNA synthetase, at 133 to 288
_00045
HEPCGN_00050: iss - increased serum survival lipoprotein Iss, at 457 to 750
iss
HEPCGN_00055: rzpD - prophage endopeptidase RzpD, at 782 to 1,243
rzpD
HEPCGN_00060: rrrD - lysozyme RrrD, at 1,240 to 1,737
rrrD
HEPCGN_00065: essD - phage lysis protein EssD, at 1,737 to 1,952
essD
HEPCGN_00070: HEPCGN_00070 - AraC family transcriptional regulator, at 2,141 to 2,872
_00070
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 0.042 kb on + strand at 0.092 kb on - strand at 0.156 kb on + strand, within HEPCGN_00045 at 0.165 kb on + strand, within HEPCGN_00045 at 0.350 kb on - strand at 0.350 kb on - strand at 0.366 kb on + strand at 0.697 kb on - strand, within iss at 0.699 kb on + strand, within iss at 0.712 kb on + strand, within iss at 0.714 kb on + strand, within iss at 0.749 kb on - strand at 0.784 kb on - strand at 0.804 kb on + strand at 0.805 kb on - strand at 1.146 kb on + strand, within rzpD at 1.150 kb on + strand, within rzpD at 1.175 kb on - strand, within rzpD at 1.319 kb on + strand, within rrrD at 1.333 kb on + strand, within rrrD at 1.472 kb on + strand, within rrrD at 1.541 kb on + strand, within rrrD at 1.605 kb on + strand, within rrrD at 1.605 kb on + strand, within rrrD at 1.614 kb on - strand, within rrrD at 1.619 kb on - strand, within rrrD at 1.619 kb on - strand, within rrrD at 1.623 kb on - strand, within rrrD at 1.678 kb on + strand, within rrrD at 1.738 kb on + strand at 1.772 kb on + strand, within essD at 1.816 kb on - strand, within essD at 1.816 kb on - strand, within essD at 1.836 kb on + strand, within essD at 1.837 kb on - strand, within essD at 1.837 kb on - strand, within essD at 1.915 kb on + strand, within essD at 1.938 kb on - strand at 2.009 kb on + strand at 2.012 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 42 + -0.2 92 - +0.1 156 + HEPCGN_00045 0.15 +0.4 165 + HEPCGN_00045 0.21 -0.1 350 - -1.0 350 - -1.0 366 + -0.5 697 - iss HEPCGN_00050 0.82 +0.6 699 + iss HEPCGN_00050 0.82 +0.7 712 + iss HEPCGN_00050 0.87 -1.2 714 + iss HEPCGN_00050 0.87 +0.1 749 - +0.8 784 - +1.0 804 + +0.1 805 - -1.8 1,146 + rzpD HEPCGN_00055 0.79 -0.5 1,150 + rzpD HEPCGN_00055 0.80 -1.2 1,175 - rzpD HEPCGN_00055 0.85 +0.1 1,319 + rrrD HEPCGN_00060 0.16 +0.2 1,333 + rrrD HEPCGN_00060 0.19 +0.1 1,472 + rrrD HEPCGN_00060 0.47 -0.1 1,541 + rrrD HEPCGN_00060 0.60 +0.3 1,605 + rrrD HEPCGN_00060 0.73 +0.2 1,605 + rrrD HEPCGN_00060 0.73 -1.2 1,614 - rrrD HEPCGN_00060 0.75 -1.0 1,619 - rrrD HEPCGN_00060 0.76 +1.3 1,619 - rrrD HEPCGN_00060 0.76 -2.2 1,623 - rrrD HEPCGN_00060 0.77 -0.3 1,678 + rrrD HEPCGN_00060 0.88 -0.1 1,738 + +0.6 1,772 + essD HEPCGN_00065 0.16 -0.8 1,816 - essD HEPCGN_00065 0.37 -0.1 1,816 - essD HEPCGN_00065 0.37 -1.3 1,836 + essD HEPCGN_00065 0.46 +0.5 1,837 - essD HEPCGN_00065 0.46 +0.9 1,837 - essD HEPCGN_00065 0.46 +0.9 1,915 + essD HEPCGN_00065 0.82 +1.4 1,938 - +2.1 2,009 + +0.1 2,012 - +0.1
Or see this region's nucleotide sequence