Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoC and nuoE are separated by 2 nucleotides nuoE and nuoF overlap by 4 nucleotides nuoF and nuoG are separated by 52 nucleotides
HEPCGN_22055: nuoC - NADH-quinone oxidoreductase subunit C/D, at 3,166,599 to 3,168,401
nuoC
HEPCGN_22060: nuoE - NADH-quinone oxidoreductase subunit NuoE, at 3,168,404 to 3,168,904
nuoE
HEPCGN_22065: nuoF - NADH-quinone oxidoreductase subunit NuoF, at 3,168,901 to 3,170,238
nuoF
HEPCGN_22070: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 3,170,291 to 3,173,017
nuoG
Position (kb)
3168
3169
3170
3171 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3167.989 kb on - strand, within nuoC at 3168.006 kb on - strand, within nuoC at 3168.132 kb on - strand, within nuoC at 3168.234 kb on + strand at 3168.234 kb on + strand at 3168.237 kb on + strand at 3168.380 kb on + strand at 3168.403 kb on + strand at 3168.403 kb on + strand at 3168.629 kb on + strand, within nuoE at 3168.698 kb on - strand, within nuoE at 3168.698 kb on - strand, within nuoE at 3168.781 kb on + strand, within nuoE at 3168.818 kb on - strand, within nuoE at 3168.896 kb on + strand at 3168.897 kb on - strand at 3168.898 kb on + strand at 3168.912 kb on + strand at 3168.985 kb on + strand at 3169.156 kb on + strand, within nuoF at 3169.157 kb on - strand, within nuoF at 3169.195 kb on + strand, within nuoF at 3169.195 kb on + strand, within nuoF at 3169.196 kb on - strand, within nuoF at 3169.196 kb on - strand, within nuoF at 3169.196 kb on - strand, within nuoF at 3169.197 kb on + strand, within nuoF at 3169.198 kb on - strand, within nuoF at 3169.246 kb on - strand, within nuoF at 3169.246 kb on - strand, within nuoF at 3169.306 kb on + strand, within nuoF at 3169.307 kb on - strand, within nuoF at 3169.307 kb on - strand, within nuoF at 3169.432 kb on + strand, within nuoF at 3169.461 kb on + strand, within nuoF at 3169.461 kb on + strand, within nuoF at 3169.462 kb on - strand, within nuoF at 3169.570 kb on - strand, within nuoF at 3169.570 kb on - strand, within nuoF at 3169.627 kb on - strand, within nuoF at 3169.963 kb on - strand, within nuoF at 3170.073 kb on - strand, within nuoF at 3170.082 kb on + strand, within nuoF at 3170.141 kb on - strand at 3170.289 kb on + strand at 3170.296 kb on + strand at 3170.296 kb on + strand at 3170.308 kb on - strand at 3170.322 kb on - strand at 3170.377 kb on + strand at 3170.384 kb on + strand at 3170.492 kb on - strand at 3170.518 kb on + strand at 3170.621 kb on - strand, within nuoG at 3170.689 kb on + strand, within nuoG at 3170.828 kb on + strand, within nuoG at 3170.829 kb on - strand, within nuoG at 3170.995 kb on + strand, within nuoG at 3170.996 kb on - strand, within nuoG at 3171.060 kb on + strand, within nuoG at 3171.068 kb on + strand, within nuoG at 3171.068 kb on + strand, within nuoG at 3171.068 kb on + strand, within nuoG at 3171.138 kb on + strand, within nuoG at 3171.202 kb on + strand, within nuoG
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 3,167,989 - nuoC HEPCGN_22055 0.77 -1.3 3,168,006 - nuoC HEPCGN_22055 0.78 -2.9 3,168,132 - nuoC HEPCGN_22055 0.85 -2.9 3,168,234 + -2.8 3,168,234 + -0.5 3,168,237 + -1.9 3,168,380 + +1.1 3,168,403 + -1.9 3,168,403 + -1.9 3,168,629 + nuoE HEPCGN_22060 0.45 -1.3 3,168,698 - nuoE HEPCGN_22060 0.59 -0.7 3,168,698 - nuoE HEPCGN_22060 0.59 +0.1 3,168,781 + nuoE HEPCGN_22060 0.75 -1.6 3,168,818 - nuoE HEPCGN_22060 0.83 -2.8 3,168,896 + -1.5 3,168,897 - -1.9 3,168,898 + -2.7 3,168,912 + -1.1 3,168,985 + -2.3 3,169,156 + nuoF HEPCGN_22065 0.19 -0.5 3,169,157 - nuoF HEPCGN_22065 0.19 +0.2 3,169,195 + nuoF HEPCGN_22065 0.22 -2.7 3,169,195 + nuoF HEPCGN_22065 0.22 -1.9 3,169,196 - nuoF HEPCGN_22065 0.22 -0.3 3,169,196 - nuoF HEPCGN_22065 0.22 -2.2 3,169,196 - nuoF HEPCGN_22065 0.22 -1.9 3,169,197 + nuoF HEPCGN_22065 0.22 -1.8 3,169,198 - nuoF HEPCGN_22065 0.22 -0.2 3,169,246 - nuoF HEPCGN_22065 0.26 -2.4 3,169,246 - nuoF HEPCGN_22065 0.26 -2.7 3,169,306 + nuoF HEPCGN_22065 0.30 -0.7 3,169,307 - nuoF HEPCGN_22065 0.30 -1.2 3,169,307 - nuoF HEPCGN_22065 0.30 -2.0 3,169,432 + nuoF HEPCGN_22065 0.40 -2.1 3,169,461 + nuoF HEPCGN_22065 0.42 -1.3 3,169,461 + nuoF HEPCGN_22065 0.42 -2.7 3,169,462 - nuoF HEPCGN_22065 0.42 +1.1 3,169,570 - nuoF HEPCGN_22065 0.50 -0.7 3,169,570 - nuoF HEPCGN_22065 0.50 -2.1 3,169,627 - nuoF HEPCGN_22065 0.54 -0.4 3,169,963 - nuoF HEPCGN_22065 0.79 -0.3 3,170,073 - nuoF HEPCGN_22065 0.88 -1.8 3,170,082 + nuoF HEPCGN_22065 0.88 -3.0 3,170,141 - -1.7 3,170,289 + -1.6 3,170,296 + -1.7 3,170,296 + -1.8 3,170,308 - -1.9 3,170,322 - +0.3 3,170,377 + -0.2 3,170,384 + -2.3 3,170,492 - -0.7 3,170,518 + -1.8 3,170,621 - nuoG HEPCGN_22070 0.12 -2.0 3,170,689 + nuoG HEPCGN_22070 0.15 -0.3 3,170,828 + nuoG HEPCGN_22070 0.20 -2.0 3,170,829 - nuoG HEPCGN_22070 0.20 -2.0 3,170,995 + nuoG HEPCGN_22070 0.26 -2.0 3,170,996 - nuoG HEPCGN_22070 0.26 +1.4 3,171,060 + nuoG HEPCGN_22070 0.28 -1.6 3,171,068 + nuoG HEPCGN_22070 0.28 -1.2 3,171,068 + nuoG HEPCGN_22070 0.28 -2.2 3,171,068 + nuoG HEPCGN_22070 0.28 -0.7 3,171,138 + nuoG HEPCGN_22070 0.31 -2.7 3,171,202 + nuoG HEPCGN_22070 0.33 -2.1
Or see this region's nucleotide sequence