Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxJ and HEPCGN_20575 overlap by 1 nucleotides HEPCGN_20575 and shoB are separated by 421 nucleotides shoB and yfhL are separated by 193 nucleotides yfhL and yfhH are separated by 55 nucleotides
HEPCGN_20570: pdxJ - pyridoxine 5'-phosphate synthase, at 2,852,633 to 2,853,364
pdxJ
HEPCGN_20575: HEPCGN_20575 - Holo-[acyl-carrier-protein] synthase, at 2,853,364 to 2,853,744
_20575
HEPCGN_20585: shoB - Small toxic protein ShoB, at 2,854,166 to 2,854,246
shoB
HEPCGN_20590: yfhL - 4Fe-4S dicluster ferredoxin YfhL, at 2,854,440 to 2,854,700
yfhL
HEPCGN_20595: yfhH - MurR/RpiR family transcriptional regulator, at 2,854,756 to 2,855,604
yfhH
Position (kb)
2854
2855 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2853.221 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.238 kb on + strand, within pdxJ at 2853.286 kb on + strand, within pdxJ at 2853.966 kb on - strand at 2853.969 kb on + strand at 2854.011 kb on + strand at 2854.120 kb on + strand at 2854.121 kb on - strand at 2854.121 kb on - strand at 2854.121 kb on - strand at 2854.128 kb on - strand at 2854.190 kb on - strand, within shoB at 2854.301 kb on + strand at 2854.301 kb on + strand at 2854.308 kb on - strand at 2854.332 kb on + strand at 2854.335 kb on - strand at 2854.335 kb on - strand at 2854.345 kb on - strand at 2854.406 kb on - strand at 2854.523 kb on + strand, within yfhL at 2854.527 kb on - strand, within yfhL at 2854.568 kb on - strand, within yfhL at 2854.575 kb on + strand, within yfhL at 2854.575 kb on + strand, within yfhL at 2854.586 kb on + strand, within yfhL at 2854.593 kb on - strand, within yfhL at 2854.614 kb on - strand, within yfhL at 2854.661 kb on + strand, within yfhL at 2854.690 kb on - strand at 2854.741 kb on - strand at 2854.794 kb on + strand at 2854.795 kb on - strand at 2854.917 kb on + strand, within yfhH at 2854.917 kb on + strand, within yfhH at 2854.918 kb on - strand, within yfhH at 2854.918 kb on - strand, within yfhH at 2854.918 kb on - strand, within yfhH at 2854.919 kb on + strand, within yfhH at 2854.920 kb on - strand, within yfhH at 2854.959 kb on + strand, within yfhH at 2854.960 kb on - strand, within yfhH at 2855.084 kb on - strand, within yfhH at 2855.088 kb on + strand, within yfhH at 2855.088 kb on + strand, within yfhH at 2855.106 kb on + strand, within yfhH at 2855.200 kb on + strand, within yfhH
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 2,853,221 + pdxJ HEPCGN_20570 0.80 +0.7 2,853,238 + pdxJ HEPCGN_20570 0.83 -1.8 2,853,238 + pdxJ HEPCGN_20570 0.83 +0.4 2,853,238 + pdxJ HEPCGN_20570 0.83 -0.7 2,853,286 + pdxJ HEPCGN_20570 0.89 -0.8 2,853,966 - -0.3 2,853,969 + +0.0 2,854,011 + +2.0 2,854,120 + +1.4 2,854,121 - +0.7 2,854,121 - -0.5 2,854,121 - -0.6 2,854,128 - +0.7 2,854,190 - shoB HEPCGN_20585 0.30 -1.1 2,854,301 + -0.8 2,854,301 + +0.2 2,854,308 - -0.5 2,854,332 + +0.5 2,854,335 - -0.8 2,854,335 - -0.0 2,854,345 - -1.0 2,854,406 - +0.0 2,854,523 + yfhL HEPCGN_20590 0.32 +1.0 2,854,527 - yfhL HEPCGN_20590 0.33 +0.4 2,854,568 - yfhL HEPCGN_20590 0.49 -0.2 2,854,575 + yfhL HEPCGN_20590 0.52 -2.4 2,854,575 + yfhL HEPCGN_20590 0.52 -0.2 2,854,586 + yfhL HEPCGN_20590 0.56 -0.6 2,854,593 - yfhL HEPCGN_20590 0.59 -0.5 2,854,614 - yfhL HEPCGN_20590 0.67 -0.0 2,854,661 + yfhL HEPCGN_20590 0.85 +0.1 2,854,690 - -0.2 2,854,741 - -0.3 2,854,794 + -1.0 2,854,795 - +0.1 2,854,917 + yfhH HEPCGN_20595 0.19 +0.4 2,854,917 + yfhH HEPCGN_20595 0.19 -0.3 2,854,918 - yfhH HEPCGN_20595 0.19 -0.4 2,854,918 - yfhH HEPCGN_20595 0.19 -0.4 2,854,918 - yfhH HEPCGN_20595 0.19 +0.4 2,854,919 + yfhH HEPCGN_20595 0.19 +0.2 2,854,920 - yfhH HEPCGN_20595 0.19 -0.3 2,854,959 + yfhH HEPCGN_20595 0.24 -0.4 2,854,960 - yfhH HEPCGN_20595 0.24 +0.1 2,855,084 - yfhH HEPCGN_20595 0.39 +0.2 2,855,088 + yfhH HEPCGN_20595 0.39 -2.7 2,855,088 + yfhH HEPCGN_20595 0.39 -1.1 2,855,106 + yfhH HEPCGN_20595 0.41 +1.5 2,855,200 + yfhH HEPCGN_20595 0.52 +0.1
Or see this region's nucleotide sequence