Strain Fitness in Escherichia coli ECOR38 around HEPCGN_20585

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxJ and HEPCGN_20575 overlap by 1 nucleotidesHEPCGN_20575 and shoB are separated by 421 nucleotidesshoB and yfhL are separated by 193 nucleotidesyfhL and yfhH are separated by 55 nucleotides HEPCGN_20570: pdxJ - pyridoxine 5'-phosphate synthase, at 2,852,633 to 2,853,364 pdxJ HEPCGN_20575: HEPCGN_20575 - Holo-[acyl-carrier-protein] synthase, at 2,853,364 to 2,853,744 _20575 HEPCGN_20585: shoB - Small toxic protein ShoB, at 2,854,166 to 2,854,246 shoB HEPCGN_20590: yfhL - 4Fe-4S dicluster ferredoxin YfhL, at 2,854,440 to 2,854,700 yfhL HEPCGN_20595: yfhH - MurR/RpiR family transcriptional regulator, at 2,854,756 to 2,855,604 yfhH Position (kb) 2854 2855Strain fitness (log2 ratio) -2 -1 0 1 2at 2853.221 kb on + strand, within pdxJat 2853.238 kb on + strand, within pdxJat 2853.238 kb on + strand, within pdxJat 2853.238 kb on + strand, within pdxJat 2853.286 kb on + strand, within pdxJat 2853.966 kb on - strandat 2853.969 kb on + strandat 2854.011 kb on + strandat 2854.120 kb on + strandat 2854.121 kb on - strandat 2854.121 kb on - strandat 2854.121 kb on - strandat 2854.128 kb on - strandat 2854.190 kb on - strand, within shoBat 2854.301 kb on + strandat 2854.301 kb on + strandat 2854.308 kb on - strandat 2854.332 kb on + strandat 2854.335 kb on - strandat 2854.335 kb on - strandat 2854.345 kb on - strandat 2854.406 kb on - strandat 2854.523 kb on + strand, within yfhLat 2854.527 kb on - strand, within yfhLat 2854.568 kb on - strand, within yfhLat 2854.575 kb on + strand, within yfhLat 2854.575 kb on + strand, within yfhLat 2854.586 kb on + strand, within yfhLat 2854.593 kb on - strand, within yfhLat 2854.614 kb on - strand, within yfhLat 2854.661 kb on + strand, within yfhLat 2854.690 kb on - strandat 2854.741 kb on - strandat 2854.794 kb on + strandat 2854.795 kb on - strandat 2854.917 kb on + strand, within yfhHat 2854.917 kb on + strand, within yfhHat 2854.918 kb on - strand, within yfhHat 2854.918 kb on - strand, within yfhHat 2854.918 kb on - strand, within yfhHat 2854.919 kb on + strand, within yfhHat 2854.920 kb on - strand, within yfhHat 2854.959 kb on + strand, within yfhHat 2854.960 kb on - strand, within yfhHat 2855.084 kb on - strand, within yfhHat 2855.088 kb on + strand, within yfhHat 2855.088 kb on + strand, within yfhHat 2855.106 kb on + strand, within yfhHat 2855.200 kb on + strand, within yfhH

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,853,221 + pdxJ HEPCGN_20570 0.80 +0.7
2,853,238 + pdxJ HEPCGN_20570 0.83 -1.8
2,853,238 + pdxJ HEPCGN_20570 0.83 +0.4
2,853,238 + pdxJ HEPCGN_20570 0.83 -0.7
2,853,286 + pdxJ HEPCGN_20570 0.89 -0.8
2,853,966 - -0.3
2,853,969 + +0.0
2,854,011 + +2.0
2,854,120 + +1.4
2,854,121 - +0.7
2,854,121 - -0.5
2,854,121 - -0.6
2,854,128 - +0.7
2,854,190 - shoB HEPCGN_20585 0.30 -1.1
2,854,301 + -0.8
2,854,301 + +0.2
2,854,308 - -0.5
2,854,332 + +0.5
2,854,335 - -0.8
2,854,335 - -0.0
2,854,345 - -1.0
2,854,406 - +0.0
2,854,523 + yfhL HEPCGN_20590 0.32 +1.0
2,854,527 - yfhL HEPCGN_20590 0.33 +0.4
2,854,568 - yfhL HEPCGN_20590 0.49 -0.2
2,854,575 + yfhL HEPCGN_20590 0.52 -2.4
2,854,575 + yfhL HEPCGN_20590 0.52 -0.2
2,854,586 + yfhL HEPCGN_20590 0.56 -0.6
2,854,593 - yfhL HEPCGN_20590 0.59 -0.5
2,854,614 - yfhL HEPCGN_20590 0.67 -0.0
2,854,661 + yfhL HEPCGN_20590 0.85 +0.1
2,854,690 - -0.2
2,854,741 - -0.3
2,854,794 + -1.0
2,854,795 - +0.1
2,854,917 + yfhH HEPCGN_20595 0.19 +0.4
2,854,917 + yfhH HEPCGN_20595 0.19 -0.3
2,854,918 - yfhH HEPCGN_20595 0.19 -0.4
2,854,918 - yfhH HEPCGN_20595 0.19 -0.4
2,854,918 - yfhH HEPCGN_20595 0.19 +0.4
2,854,919 + yfhH HEPCGN_20595 0.19 +0.2
2,854,920 - yfhH HEPCGN_20595 0.19 -0.3
2,854,959 + yfhH HEPCGN_20595 0.24 -0.4
2,854,960 - yfhH HEPCGN_20595 0.24 +0.1
2,855,084 - yfhH HEPCGN_20595 0.39 +0.2
2,855,088 + yfhH HEPCGN_20595 0.39 -2.7
2,855,088 + yfhH HEPCGN_20595 0.39 -1.1
2,855,106 + yfhH HEPCGN_20595 0.41 +1.5
2,855,200 + yfhH HEPCGN_20595 0.52 +0.1

Or see this region's nucleotide sequence