Strain Fitness in Escherichia coli ECOR38 around HEPCGN_19900

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmltB and pncC are separated by 144 nucleotidespncC and recA are separated by 79 nucleotidesrecA and recX are separated by 68 nucleotidesrecX and alaS are separated by 128 nucleotides HEPCGN_19890: mltB - lytic murein transglycosylase B, at 2,731,848 to 2,733,038 mltB HEPCGN_19895: pncC - nicotinamide-nucleotide amidase, at 2,733,183 to 2,733,680 pncC HEPCGN_19900: recA - recombinase RecA, at 2,733,760 to 2,734,821 recA HEPCGN_19905: recX - recombination regulator RecX, at 2,734,890 to 2,735,390 recX HEPCGN_19910: alaS - alanine--tRNA ligase, at 2,735,519 to 2,738,149 alaS Position (kb) 2733 2734 2735Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2732.820 kb on + strand, within mltBat 2732.851 kb on + strand, within mltBat 2732.966 kb on + strandat 2732.967 kb on - strandat 2732.967 kb on - strandat 2733.036 kb on + strandat 2733.040 kb on + strandat 2733.040 kb on + strandat 2733.040 kb on + strandat 2733.041 kb on - strandat 2733.041 kb on - strandat 2733.144 kb on - strandat 2733.348 kb on + strand, within pncCat 2733.474 kb on + strand, within pncCat 2733.486 kb on - strand, within pncCat 2733.576 kb on - strand, within pncCat 2733.579 kb on - strand, within pncCat 2733.636 kb on + strandat 2733.637 kb on - strandat 2733.637 kb on - strandat 2733.637 kb on - strandat 2733.637 kb on - strandat 2733.637 kb on - strandat 2733.701 kb on + strandat 2733.720 kb on + strandat 2733.720 kb on + strandat 2733.955 kb on + strand, within recAat 2734.023 kb on + strand, within recAat 2734.069 kb on + strand, within recAat 2734.069 kb on + strand, within recAat 2734.069 kb on + strand, within recAat 2734.070 kb on - strand, within recAat 2734.270 kb on - strand, within recAat 2734.270 kb on - strand, within recAat 2734.414 kb on + strand, within recAat 2734.444 kb on - strand, within recAat 2734.574 kb on - strand, within recAat 2734.730 kb on + strandat 2734.778 kb on + strandat 2734.836 kb on - strandat 2734.871 kb on + strandat 2734.878 kb on + strandat 2734.884 kb on + strandat 2734.884 kb on + strandat 2734.884 kb on + strandat 2734.885 kb on - strandat 2734.921 kb on - strandat 2735.091 kb on + strand, within recXat 2735.091 kb on + strand, within recXat 2735.092 kb on - strand, within recXat 2735.366 kb on + strandat 2735.449 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,732,820 + mltB HEPCGN_19890 0.82 +0.4
2,732,851 + mltB HEPCGN_19890 0.84 -0.0
2,732,966 + +0.9
2,732,967 - +0.9
2,732,967 - +1.9
2,733,036 + +2.1
2,733,040 + +0.4
2,733,040 + +0.4
2,733,040 + -0.6
2,733,041 - -0.3
2,733,041 - -1.7
2,733,144 - -0.3
2,733,348 + pncC HEPCGN_19895 0.33 -0.5
2,733,474 + pncC HEPCGN_19895 0.58 +1.4
2,733,486 - pncC HEPCGN_19895 0.61 +0.2
2,733,576 - pncC HEPCGN_19895 0.79 +1.3
2,733,579 - pncC HEPCGN_19895 0.80 +0.5
2,733,636 + +2.9
2,733,637 - -0.3
2,733,637 - -0.9
2,733,637 - -0.4
2,733,637 - -0.4
2,733,637 - -0.1
2,733,701 + +0.0
2,733,720 + +0.6
2,733,720 + +0.3
2,733,955 + recA HEPCGN_19900 0.18 +0.6
2,734,023 + recA HEPCGN_19900 0.25 +2.9
2,734,069 + recA HEPCGN_19900 0.29 +0.2
2,734,069 + recA HEPCGN_19900 0.29 -1.2
2,734,069 + recA HEPCGN_19900 0.29 -1.4
2,734,070 - recA HEPCGN_19900 0.29 +0.1
2,734,270 - recA HEPCGN_19900 0.48 -2.0
2,734,270 - recA HEPCGN_19900 0.48 -1.4
2,734,414 + recA HEPCGN_19900 0.62 -0.5
2,734,444 - recA HEPCGN_19900 0.64 -0.6
2,734,574 - recA HEPCGN_19900 0.77 +0.3
2,734,730 + -0.6
2,734,778 + -0.2
2,734,836 - +0.1
2,734,871 + -0.1
2,734,878 + -0.5
2,734,884 + +0.2
2,734,884 + +1.1
2,734,884 + +0.2
2,734,885 - -0.4
2,734,921 - -0.6
2,735,091 + recX HEPCGN_19905 0.40 -0.6
2,735,091 + recX HEPCGN_19905 0.40 -0.3
2,735,092 - recX HEPCGN_19905 0.40 +0.3
2,735,366 + +0.0
2,735,449 + +0.2

Or see this region's nucleotide sequence