Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycA and hycB are separated by 124 nucleotides hycB and hycC overlap by 4 nucleotides hycC and hycD are separated by 2 nucleotides hycD and hycE are separated by 17 nucleotides
HEPCGN_19760: hycA - formate hydrogenlyase regulator HycA, at 2,705,720 to 2,706,181
hycA
HEPCGN_19765: hycB - formate hydrogenlyase subunit HycB, at 2,706,306 to 2,706,917
hycB
HEPCGN_19770: hycC - formate hydrogenlyase subunit 3, at 2,706,914 to 2,708,740
hycC
HEPCGN_19775: hycD - formate hydrogenlyase subunit HycD, at 2,708,743 to 2,709,666
hycD
HEPCGN_19780: hycE - formate hydrogenlyase subunit HycE, at 2,709,684 to 2,711,393
hycE
Position (kb)
2706
2707
2708
2709 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2705.957 kb on + strand, within hycA at 2706.074 kb on + strand, within hycA at 2706.075 kb on - strand, within hycA at 2706.108 kb on + strand, within hycA at 2706.108 kb on + strand, within hycA at 2706.108 kb on + strand, within hycA at 2706.108 kb on + strand, within hycA at 2706.108 kb on + strand, within hycA at 2706.109 kb on - strand, within hycA at 2706.109 kb on - strand, within hycA at 2706.109 kb on - strand, within hycA at 2706.109 kb on - strand, within hycA at 2706.109 kb on - strand, within hycA at 2706.111 kb on - strand, within hycA at 2706.112 kb on + strand, within hycA at 2706.156 kb on + strand at 2706.554 kb on - strand, within hycB at 2706.554 kb on - strand, within hycB at 2706.710 kb on + strand, within hycB at 2706.839 kb on + strand, within hycB at 2706.839 kb on + strand, within hycB at 2706.857 kb on + strand at 2706.857 kb on + strand at 2706.911 kb on + strand at 2706.912 kb on - strand at 2707.047 kb on - strand at 2707.119 kb on - strand, within hycC at 2707.159 kb on + strand, within hycC at 2707.159 kb on + strand, within hycC at 2707.194 kb on - strand, within hycC at 2707.249 kb on + strand, within hycC at 2707.250 kb on - strand, within hycC at 2707.250 kb on - strand, within hycC at 2707.308 kb on + strand, within hycC at 2707.474 kb on + strand, within hycC at 2707.620 kb on + strand, within hycC at 2707.651 kb on - strand, within hycC at 2707.759 kb on + strand, within hycC at 2707.759 kb on + strand, within hycC at 2707.759 kb on + strand, within hycC at 2707.759 kb on + strand, within hycC at 2707.759 kb on + strand, within hycC at 2707.910 kb on - strand, within hycC at 2708.015 kb on - strand, within hycC at 2708.092 kb on + strand, within hycC at 2708.093 kb on - strand, within hycC at 2708.096 kb on + strand, within hycC at 2708.096 kb on + strand, within hycC at 2708.203 kb on + strand, within hycC at 2708.203 kb on + strand, within hycC at 2708.292 kb on + strand, within hycC at 2708.292 kb on + strand, within hycC at 2708.293 kb on - strand, within hycC at 2708.322 kb on + strand, within hycC at 2708.374 kb on - strand, within hycC at 2708.396 kb on - strand, within hycC at 2708.754 kb on - strand at 2708.754 kb on - strand at 2708.839 kb on - strand, within hycD at 2708.870 kb on - strand, within hycD at 2708.947 kb on + strand, within hycD at 2709.041 kb on + strand, within hycD at 2709.041 kb on + strand, within hycD at 2709.042 kb on - strand, within hycD at 2709.042 kb on - strand, within hycD at 2709.235 kb on + strand, within hycD at 2709.597 kb on + strand at 2709.598 kb on - strand at 2709.609 kb on + strand at 2709.639 kb on - strand at 2709.711 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 2,705,957 + hycA HEPCGN_19760 0.51 +0.3 2,706,074 + hycA HEPCGN_19760 0.77 +0.7 2,706,075 - hycA HEPCGN_19760 0.77 +0.0 2,706,108 + hycA HEPCGN_19760 0.84 +0.2 2,706,108 + hycA HEPCGN_19760 0.84 +0.5 2,706,108 + hycA HEPCGN_19760 0.84 -1.6 2,706,108 + hycA HEPCGN_19760 0.84 -1.9 2,706,108 + hycA HEPCGN_19760 0.84 -0.3 2,706,109 - hycA HEPCGN_19760 0.84 +0.5 2,706,109 - hycA HEPCGN_19760 0.84 -0.9 2,706,109 - hycA HEPCGN_19760 0.84 -0.3 2,706,109 - hycA HEPCGN_19760 0.84 +0.7 2,706,109 - hycA HEPCGN_19760 0.84 +0.9 2,706,111 - hycA HEPCGN_19760 0.85 -0.1 2,706,112 + hycA HEPCGN_19760 0.85 +0.6 2,706,156 + +0.2 2,706,554 - hycB HEPCGN_19765 0.41 -0.7 2,706,554 - hycB HEPCGN_19765 0.41 -0.5 2,706,710 + hycB HEPCGN_19765 0.66 -0.2 2,706,839 + hycB HEPCGN_19765 0.87 +0.0 2,706,839 + hycB HEPCGN_19765 0.87 +0.1 2,706,857 + +0.2 2,706,857 + +0.3 2,706,911 + +0.8 2,706,912 - -0.2 2,707,047 - -0.5 2,707,119 - hycC HEPCGN_19770 0.11 +1.2 2,707,159 + hycC HEPCGN_19770 0.13 -0.0 2,707,159 + hycC HEPCGN_19770 0.13 -1.4 2,707,194 - hycC HEPCGN_19770 0.15 +0.1 2,707,249 + hycC HEPCGN_19770 0.18 -0.6 2,707,250 - hycC HEPCGN_19770 0.18 +0.5 2,707,250 - hycC HEPCGN_19770 0.18 -1.1 2,707,308 + hycC HEPCGN_19770 0.22 +0.2 2,707,474 + hycC HEPCGN_19770 0.31 +0.3 2,707,620 + hycC HEPCGN_19770 0.39 +0.5 2,707,651 - hycC HEPCGN_19770 0.40 -1.0 2,707,759 + hycC HEPCGN_19770 0.46 +2.5 2,707,759 + hycC HEPCGN_19770 0.46 +1.1 2,707,759 + hycC HEPCGN_19770 0.46 +0.4 2,707,759 + hycC HEPCGN_19770 0.46 +0.3 2,707,759 + hycC HEPCGN_19770 0.46 +0.9 2,707,910 - hycC HEPCGN_19770 0.55 +0.0 2,708,015 - hycC HEPCGN_19770 0.60 -0.4 2,708,092 + hycC HEPCGN_19770 0.64 -0.5 2,708,093 - hycC HEPCGN_19770 0.65 +0.3 2,708,096 + hycC HEPCGN_19770 0.65 +0.1 2,708,096 + hycC HEPCGN_19770 0.65 -1.0 2,708,203 + hycC HEPCGN_19770 0.71 +0.4 2,708,203 + hycC HEPCGN_19770 0.71 -0.6 2,708,292 + hycC HEPCGN_19770 0.75 -0.5 2,708,292 + hycC HEPCGN_19770 0.75 +1.0 2,708,293 - hycC HEPCGN_19770 0.75 +1.0 2,708,322 + hycC HEPCGN_19770 0.77 -0.5 2,708,374 - hycC HEPCGN_19770 0.80 +0.9 2,708,396 - hycC HEPCGN_19770 0.81 +0.4 2,708,754 - -3.5 2,708,754 - -0.2 2,708,839 - hycD HEPCGN_19775 0.10 +0.3 2,708,870 - hycD HEPCGN_19775 0.14 +0.3 2,708,947 + hycD HEPCGN_19775 0.22 -0.3 2,709,041 + hycD HEPCGN_19775 0.32 +0.9 2,709,041 + hycD HEPCGN_19775 0.32 -2.2 2,709,042 - hycD HEPCGN_19775 0.32 +0.0 2,709,042 - hycD HEPCGN_19775 0.32 +0.2 2,709,235 + hycD HEPCGN_19775 0.53 -0.5 2,709,597 + +0.3 2,709,598 - -0.4 2,709,609 + -1.6 2,709,639 - +0.9 2,709,711 + -0.7
Or see this region's nucleotide sequence