Strain Fitness in Escherichia coli ECOR38 around HEPCGN_19770

Experiment: K25

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycA and hycB are separated by 124 nucleotideshycB and hycC overlap by 4 nucleotideshycC and hycD are separated by 2 nucleotideshycD and hycE are separated by 17 nucleotides HEPCGN_19760: hycA - formate hydrogenlyase regulator HycA, at 2,705,720 to 2,706,181 hycA HEPCGN_19765: hycB - formate hydrogenlyase subunit HycB, at 2,706,306 to 2,706,917 hycB HEPCGN_19770: hycC - formate hydrogenlyase subunit 3, at 2,706,914 to 2,708,740 hycC HEPCGN_19775: hycD - formate hydrogenlyase subunit HycD, at 2,708,743 to 2,709,666 hycD HEPCGN_19780: hycE - formate hydrogenlyase subunit HycE, at 2,709,684 to 2,711,393 hycE Position (kb) 2706 2707 2708 2709Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2705.957 kb on + strand, within hycAat 2706.074 kb on + strand, within hycAat 2706.075 kb on - strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.108 kb on + strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.109 kb on - strand, within hycAat 2706.111 kb on - strand, within hycAat 2706.112 kb on + strand, within hycAat 2706.156 kb on + strandat 2706.554 kb on - strand, within hycBat 2706.554 kb on - strand, within hycBat 2706.710 kb on + strand, within hycBat 2706.839 kb on + strand, within hycBat 2706.839 kb on + strand, within hycBat 2706.857 kb on + strandat 2706.857 kb on + strandat 2706.911 kb on + strandat 2706.912 kb on - strandat 2707.047 kb on - strandat 2707.119 kb on - strand, within hycCat 2707.159 kb on + strand, within hycCat 2707.159 kb on + strand, within hycCat 2707.194 kb on - strand, within hycCat 2707.249 kb on + strand, within hycCat 2707.250 kb on - strand, within hycCat 2707.250 kb on - strand, within hycCat 2707.308 kb on + strand, within hycCat 2707.474 kb on + strand, within hycCat 2707.620 kb on + strand, within hycCat 2707.651 kb on - strand, within hycCat 2707.759 kb on + strand, within hycCat 2707.759 kb on + strand, within hycCat 2707.759 kb on + strand, within hycCat 2707.759 kb on + strand, within hycCat 2707.759 kb on + strand, within hycCat 2707.910 kb on - strand, within hycCat 2708.015 kb on - strand, within hycCat 2708.092 kb on + strand, within hycCat 2708.093 kb on - strand, within hycCat 2708.096 kb on + strand, within hycCat 2708.096 kb on + strand, within hycCat 2708.203 kb on + strand, within hycCat 2708.203 kb on + strand, within hycCat 2708.292 kb on + strand, within hycCat 2708.292 kb on + strand, within hycCat 2708.293 kb on - strand, within hycCat 2708.322 kb on + strand, within hycCat 2708.374 kb on - strand, within hycCat 2708.396 kb on - strand, within hycCat 2708.754 kb on - strandat 2708.754 kb on - strandat 2708.839 kb on - strand, within hycDat 2708.870 kb on - strand, within hycDat 2708.947 kb on + strand, within hycDat 2709.041 kb on + strand, within hycDat 2709.041 kb on + strand, within hycDat 2709.042 kb on - strand, within hycDat 2709.042 kb on - strand, within hycDat 2709.235 kb on + strand, within hycDat 2709.597 kb on + strandat 2709.598 kb on - strandat 2709.609 kb on + strandat 2709.639 kb on - strandat 2709.711 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K25
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2,705,957 + hycA HEPCGN_19760 0.51 +0.3
2,706,074 + hycA HEPCGN_19760 0.77 +0.7
2,706,075 - hycA HEPCGN_19760 0.77 +0.0
2,706,108 + hycA HEPCGN_19760 0.84 +0.2
2,706,108 + hycA HEPCGN_19760 0.84 +0.5
2,706,108 + hycA HEPCGN_19760 0.84 -1.6
2,706,108 + hycA HEPCGN_19760 0.84 -1.9
2,706,108 + hycA HEPCGN_19760 0.84 -0.3
2,706,109 - hycA HEPCGN_19760 0.84 +0.5
2,706,109 - hycA HEPCGN_19760 0.84 -0.9
2,706,109 - hycA HEPCGN_19760 0.84 -0.3
2,706,109 - hycA HEPCGN_19760 0.84 +0.7
2,706,109 - hycA HEPCGN_19760 0.84 +0.9
2,706,111 - hycA HEPCGN_19760 0.85 -0.1
2,706,112 + hycA HEPCGN_19760 0.85 +0.6
2,706,156 + +0.2
2,706,554 - hycB HEPCGN_19765 0.41 -0.7
2,706,554 - hycB HEPCGN_19765 0.41 -0.5
2,706,710 + hycB HEPCGN_19765 0.66 -0.2
2,706,839 + hycB HEPCGN_19765 0.87 +0.0
2,706,839 + hycB HEPCGN_19765 0.87 +0.1
2,706,857 + +0.2
2,706,857 + +0.3
2,706,911 + +0.8
2,706,912 - -0.2
2,707,047 - -0.5
2,707,119 - hycC HEPCGN_19770 0.11 +1.2
2,707,159 + hycC HEPCGN_19770 0.13 -0.0
2,707,159 + hycC HEPCGN_19770 0.13 -1.4
2,707,194 - hycC HEPCGN_19770 0.15 +0.1
2,707,249 + hycC HEPCGN_19770 0.18 -0.6
2,707,250 - hycC HEPCGN_19770 0.18 +0.5
2,707,250 - hycC HEPCGN_19770 0.18 -1.1
2,707,308 + hycC HEPCGN_19770 0.22 +0.2
2,707,474 + hycC HEPCGN_19770 0.31 +0.3
2,707,620 + hycC HEPCGN_19770 0.39 +0.5
2,707,651 - hycC HEPCGN_19770 0.40 -1.0
2,707,759 + hycC HEPCGN_19770 0.46 +2.5
2,707,759 + hycC HEPCGN_19770 0.46 +1.1
2,707,759 + hycC HEPCGN_19770 0.46 +0.4
2,707,759 + hycC HEPCGN_19770 0.46 +0.3
2,707,759 + hycC HEPCGN_19770 0.46 +0.9
2,707,910 - hycC HEPCGN_19770 0.55 +0.0
2,708,015 - hycC HEPCGN_19770 0.60 -0.4
2,708,092 + hycC HEPCGN_19770 0.64 -0.5
2,708,093 - hycC HEPCGN_19770 0.65 +0.3
2,708,096 + hycC HEPCGN_19770 0.65 +0.1
2,708,096 + hycC HEPCGN_19770 0.65 -1.0
2,708,203 + hycC HEPCGN_19770 0.71 +0.4
2,708,203 + hycC HEPCGN_19770 0.71 -0.6
2,708,292 + hycC HEPCGN_19770 0.75 -0.5
2,708,292 + hycC HEPCGN_19770 0.75 +1.0
2,708,293 - hycC HEPCGN_19770 0.75 +1.0
2,708,322 + hycC HEPCGN_19770 0.77 -0.5
2,708,374 - hycC HEPCGN_19770 0.80 +0.9
2,708,396 - hycC HEPCGN_19770 0.81 +0.4
2,708,754 - -3.5
2,708,754 - -0.2
2,708,839 - hycD HEPCGN_19775 0.10 +0.3
2,708,870 - hycD HEPCGN_19775 0.14 +0.3
2,708,947 + hycD HEPCGN_19775 0.22 -0.3
2,709,041 + hycD HEPCGN_19775 0.32 +0.9
2,709,041 + hycD HEPCGN_19775 0.32 -2.2
2,709,042 - hycD HEPCGN_19775 0.32 +0.0
2,709,042 - hycD HEPCGN_19775 0.32 +0.2
2,709,235 + hycD HEPCGN_19775 0.53 -0.5
2,709,597 + +0.3
2,709,598 - -0.4
2,709,609 + -1.6
2,709,639 - +0.9
2,709,711 + -0.7

Or see this region's nucleotide sequence