Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygfZ and sdhE are separated by 242 nucleotides sdhE and ygfX overlap by 20 nucleotides ygfX and fldB are separated by 39 nucleotides
HEPCGN_18840: ygfZ - tRNA-modifying protein YgfZ, at 2,512,887 to 2,513,867
ygfZ
HEPCGN_18845: sdhE - FAD assembly factor SdhE, at 2,514,110 to 2,514,376
sdhE
HEPCGN_18850: ygfX - Inner membrane protein YgfX, at 2,514,357 to 2,514,764
ygfX
HEPCGN_18855: fldB - flavodoxin FldB, at 2,514,804 to 2,515,325
fldB
Position (kb)
2514
2515 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2513.871 kb on - strand at 2513.871 kb on - strand at 2513.871 kb on - strand at 2513.871 kb on - strand at 2513.871 kb on - strand at 2513.871 kb on - strand at 2513.898 kb on + strand at 2513.920 kb on + strand at 2513.920 kb on + strand at 2514.070 kb on - strand at 2514.074 kb on + strand at 2514.119 kb on - strand at 2514.119 kb on - strand at 2514.119 kb on - strand at 2514.203 kb on + strand, within sdhE at 2514.203 kb on + strand, within sdhE at 2514.440 kb on + strand, within ygfX at 2514.445 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.490 kb on + strand, within ygfX at 2514.762 kb on + strand at 2514.781 kb on + strand at 2514.781 kb on + strand at 2514.782 kb on - strand at 2514.782 kb on - strand at 2514.782 kb on - strand at 2514.857 kb on + strand, within fldB at 2514.857 kb on + strand, within fldB at 2514.884 kb on + strand, within fldB at 2514.948 kb on - strand, within fldB at 2514.948 kb on - strand, within fldB at 2515.019 kb on + strand, within fldB at 2515.088 kb on + strand, within fldB at 2515.189 kb on + strand, within fldB at 2515.227 kb on - strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.230 kb on + strand, within fldB at 2515.231 kb on - strand, within fldB at 2515.289 kb on - strand at 2515.289 kb on - strand at 2515.320 kb on - strand at 2515.320 kb on - strand at 2515.320 kb on - strand at 2515.325 kb on + strand at 2515.326 kb on - strand at 2515.330 kb on + strand at 2515.363 kb on + strand at 2515.363 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 2,513,871 - -0.6 2,513,871 - -0.9 2,513,871 - -0.8 2,513,871 - -0.0 2,513,871 - -1.1 2,513,871 - -0.8 2,513,898 + +0.6 2,513,920 + +0.5 2,513,920 + -0.4 2,514,070 - -1.1 2,514,074 + -0.7 2,514,119 - -2.2 2,514,119 - +0.2 2,514,119 - -0.4 2,514,203 + sdhE HEPCGN_18845 0.35 -2.1 2,514,203 + sdhE HEPCGN_18845 0.35 +0.4 2,514,440 + ygfX HEPCGN_18850 0.20 +0.3 2,514,445 + ygfX HEPCGN_18850 0.22 -0.5 2,514,490 + ygfX HEPCGN_18850 0.33 -0.1 2,514,490 + ygfX HEPCGN_18850 0.33 +0.3 2,514,490 + ygfX HEPCGN_18850 0.33 -1.1 2,514,762 + -0.9 2,514,781 + +0.2 2,514,781 + +0.1 2,514,782 - -1.0 2,514,782 - -2.3 2,514,782 - -0.3 2,514,857 + fldB HEPCGN_18855 0.10 +0.4 2,514,857 + fldB HEPCGN_18855 0.10 -0.3 2,514,884 + fldB HEPCGN_18855 0.15 +0.6 2,514,948 - fldB HEPCGN_18855 0.28 -1.8 2,514,948 - fldB HEPCGN_18855 0.28 +0.5 2,515,019 + fldB HEPCGN_18855 0.41 +0.5 2,515,088 + fldB HEPCGN_18855 0.54 -0.5 2,515,189 + fldB HEPCGN_18855 0.74 +3.0 2,515,227 - fldB HEPCGN_18855 0.81 -2.4 2,515,230 + fldB HEPCGN_18855 0.82 -0.3 2,515,230 + fldB HEPCGN_18855 0.82 +0.2 2,515,230 + fldB HEPCGN_18855 0.82 +0.4 2,515,230 + fldB HEPCGN_18855 0.82 +0.4 2,515,230 + fldB HEPCGN_18855 0.82 -1.8 2,515,230 + fldB HEPCGN_18855 0.82 -1.7 2,515,231 - fldB HEPCGN_18855 0.82 +1.0 2,515,289 - +0.8 2,515,289 - -0.4 2,515,320 - -0.8 2,515,320 - -0.6 2,515,320 - -0.6 2,515,325 + -0.7 2,515,326 - -1.5 2,515,330 + -0.3 2,515,363 + -2.3 2,515,363 + -0.7
Or see this region's nucleotide sequence