Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zapA and ygfB are separated by 167 nucleotides ygfB and pepP are separated by 25 nucleotides pepP and ubiH overlap by 4 nucleotides
HEPCGN_18785: zapA - cell division protein ZapA, at 2,500,260 to 2,500,589
zapA
HEPCGN_18790: ygfB - UPF0149 family protein YgfB, at 2,500,757 to 2,501,335
ygfB
HEPCGN_18795: pepP - Xaa-Pro aminopeptidase, at 2,501,361 to 2,502,686
pepP
HEPCGN_18800: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 2,502,683 to 2,503,861
ubiH
Position (kb)
2501
2502
2503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2500.395 kb on + strand, within zapA at 2500.402 kb on + strand, within zapA at 2500.402 kb on + strand, within zapA at 2500.403 kb on - strand, within zapA at 2500.403 kb on - strand, within zapA at 2500.442 kb on + strand, within zapA at 2500.443 kb on - strand, within zapA at 2500.658 kb on - strand at 2500.659 kb on + strand at 2500.659 kb on + strand at 2500.659 kb on + strand at 2500.659 kb on + strand at 2500.660 kb on - strand at 2500.663 kb on + strand at 2500.664 kb on - strand at 2500.716 kb on + strand at 2500.721 kb on + strand at 2500.721 kb on + strand at 2500.721 kb on + strand at 2500.721 kb on + strand at 2500.722 kb on - strand at 2500.722 kb on - strand at 2500.731 kb on - strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.756 kb on + strand at 2500.757 kb on - strand at 2500.757 kb on - strand at 2500.762 kb on + strand at 2500.762 kb on + strand at 2500.785 kb on - strand at 2500.866 kb on + strand, within ygfB at 2500.867 kb on - strand, within ygfB at 2500.867 kb on - strand, within ygfB at 2500.867 kb on - strand, within ygfB at 2500.963 kb on + strand, within ygfB at 2501.170 kb on + strand, within ygfB at 2501.246 kb on + strand, within ygfB at 2501.246 kb on + strand, within ygfB at 2501.247 kb on - strand, within ygfB at 2501.247 kb on - strand, within ygfB at 2501.269 kb on - strand, within ygfB at 2501.285 kb on - strand at 2501.360 kb on + strand at 2501.722 kb on + strand, within pepP at 2501.722 kb on + strand, within pepP at 2501.745 kb on + strand, within pepP at 2501.769 kb on + strand, within pepP at 2501.911 kb on + strand, within pepP at 2501.911 kb on + strand, within pepP at 2502.050 kb on + strand, within pepP at 2502.050 kb on + strand, within pepP at 2502.051 kb on - strand, within pepP at 2502.340 kb on + strand, within pepP at 2502.430 kb on + strand, within pepP at 2502.499 kb on + strand, within pepP at 2502.522 kb on + strand, within pepP at 2502.522 kb on + strand, within pepP
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 2,500,395 + zapA HEPCGN_18785 0.41 -0.0 2,500,402 + zapA HEPCGN_18785 0.43 +1.1 2,500,402 + zapA HEPCGN_18785 0.43 +0.6 2,500,403 - zapA HEPCGN_18785 0.43 +1.6 2,500,403 - zapA HEPCGN_18785 0.43 -2.1 2,500,442 + zapA HEPCGN_18785 0.55 +1.0 2,500,443 - zapA HEPCGN_18785 0.55 +1.2 2,500,658 - +0.3 2,500,659 + +0.3 2,500,659 + -2.4 2,500,659 + -1.4 2,500,659 + +1.6 2,500,660 - +0.4 2,500,663 + +0.9 2,500,664 - +0.2 2,500,716 + -0.8 2,500,721 + -0.3 2,500,721 + -0.1 2,500,721 + +0.6 2,500,721 + +0.6 2,500,722 - -0.6 2,500,722 - -0.6 2,500,731 - -0.9 2,500,756 + +0.2 2,500,756 + +0.2 2,500,756 + -0.5 2,500,756 + -0.1 2,500,756 + -0.3 2,500,756 + -1.0 2,500,756 + +1.1 2,500,756 + -0.1 2,500,756 + -0.9 2,500,757 - -2.7 2,500,757 - -3.3 2,500,762 + +1.1 2,500,762 + -0.9 2,500,785 - -0.8 2,500,866 + ygfB HEPCGN_18790 0.19 -0.0 2,500,867 - ygfB HEPCGN_18790 0.19 -0.3 2,500,867 - ygfB HEPCGN_18790 0.19 +0.6 2,500,867 - ygfB HEPCGN_18790 0.19 -1.2 2,500,963 + ygfB HEPCGN_18790 0.36 -0.6 2,501,170 + ygfB HEPCGN_18790 0.71 -0.5 2,501,246 + ygfB HEPCGN_18790 0.84 +2.4 2,501,246 + ygfB HEPCGN_18790 0.84 -0.2 2,501,247 - ygfB HEPCGN_18790 0.85 -0.8 2,501,247 - ygfB HEPCGN_18790 0.85 -2.2 2,501,269 - ygfB HEPCGN_18790 0.88 -1.6 2,501,285 - -1.6 2,501,360 + -0.4 2,501,722 + pepP HEPCGN_18795 0.27 -1.1 2,501,722 + pepP HEPCGN_18795 0.27 +2.1 2,501,745 + pepP HEPCGN_18795 0.29 -0.3 2,501,769 + pepP HEPCGN_18795 0.31 -0.2 2,501,911 + pepP HEPCGN_18795 0.41 -2.2 2,501,911 + pepP HEPCGN_18795 0.41 -2.0 2,502,050 + pepP HEPCGN_18795 0.52 -2.0 2,502,050 + pepP HEPCGN_18795 0.52 +2.0 2,502,051 - pepP HEPCGN_18795 0.52 -2.3 2,502,340 + pepP HEPCGN_18795 0.74 +0.6 2,502,430 + pepP HEPCGN_18795 0.81 +0.2 2,502,499 + pepP HEPCGN_18795 0.86 -0.7 2,502,522 + pepP HEPCGN_18795 0.88 -1.8 2,502,522 + pepP HEPCGN_18795 0.88 -0.4
Or see this region's nucleotide sequence