Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt damX and dam are separated by 106 nucleotides dam and rpe are separated by 17 nucleotides rpe and gph overlap by 8 nucleotides gph and trpS overlap by 8 nucleotides
HEPCGN_15940: damX - cell division protein DamX, at 1,961,815 to 1,963,101
damX
HEPCGN_15945: dam - adenine-specific DNA-methyltransferase, at 1,963,208 to 1,964,044
dam
HEPCGN_15950: rpe - ribulose-phosphate 3-epimerase, at 1,964,062 to 1,964,739
rpe
HEPCGN_15955: gph - phosphoglycolate phosphatase, at 1,964,732 to 1,965,490
gph
HEPCGN_15960: trpS - tryptophan--tRNA ligase, at 1,965,483 to 1,966,487
trpS
Position (kb)
1964
1965 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1963.082 kb on + strand at 1963.099 kb on + strand at 1963.099 kb on + strand at 1963.099 kb on + strand at 1963.127 kb on - strand at 1963.193 kb on - strand at 1963.193 kb on - strand at 1963.196 kb on + strand at 1963.197 kb on - strand at 1963.197 kb on - strand at 1963.197 kb on - strand at 1963.251 kb on - strand at 1963.270 kb on + strand at 1963.271 kb on - strand at 1963.314 kb on + strand, within dam at 1963.315 kb on - strand, within dam at 1963.355 kb on + strand, within dam at 1963.356 kb on - strand, within dam at 1963.428 kb on + strand, within dam at 1963.429 kb on - strand, within dam at 1963.478 kb on + strand, within dam at 1963.581 kb on - strand, within dam at 1963.688 kb on + strand, within dam at 1963.742 kb on + strand, within dam at 1963.957 kb on + strand, within dam at 1963.960 kb on - strand, within dam at 1963.981 kb on + strand at 1964.233 kb on - strand, within rpe at 1964.328 kb on + strand, within rpe at 1964.328 kb on + strand, within rpe at 1964.434 kb on + strand, within rpe at 1964.553 kb on + strand, within rpe at 1964.643 kb on + strand, within rpe at 1964.726 kb on + strand at 1964.726 kb on + strand at 1964.738 kb on - strand at 1964.779 kb on + strand at 1964.780 kb on - strand at 1964.817 kb on + strand, within gph at 1964.817 kb on + strand, within gph at 1964.817 kb on + strand, within gph at 1964.818 kb on - strand, within gph at 1964.818 kb on - strand, within gph at 1964.821 kb on + strand, within gph at 1964.822 kb on - strand, within gph at 1964.822 kb on - strand, within gph at 1964.871 kb on + strand, within gph at 1964.872 kb on - strand, within gph at 1964.872 kb on - strand, within gph at 1964.872 kb on - strand, within gph at 1964.872 kb on - strand, within gph at 1964.963 kb on - strand, within gph at 1965.007 kb on + strand, within gph at 1965.008 kb on - strand, within gph at 1965.008 kb on - strand, within gph at 1965.008 kb on - strand, within gph at 1965.033 kb on - strand, within gph at 1965.121 kb on - strand, within gph at 1965.121 kb on - strand, within gph at 1965.174 kb on + strand, within gph at 1965.174 kb on + strand, within gph at 1965.188 kb on + strand, within gph at 1965.189 kb on - strand, within gph at 1965.252 kb on + strand, within gph at 1965.253 kb on - strand, within gph at 1965.319 kb on + strand, within gph at 1965.320 kb on - strand, within gph at 1965.372 kb on - strand, within gph at 1965.373 kb on + strand, within gph at 1965.373 kb on + strand, within gph at 1965.374 kb on - strand, within gph at 1965.374 kb on - strand, within gph at 1965.378 kb on - strand, within gph at 1965.378 kb on - strand, within gph at 1965.378 kb on - strand, within gph at 1965.411 kb on + strand, within gph at 1965.424 kb on + strand at 1965.426 kb on + strand at 1965.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 1,963,082 + +0.0 1,963,099 + -2.0 1,963,099 + +0.0 1,963,099 + -0.0 1,963,127 - -0.9 1,963,193 - -0.9 1,963,193 - +0.6 1,963,196 + -0.2 1,963,197 - -1.5 1,963,197 - +0.1 1,963,197 - -1.5 1,963,251 - -0.2 1,963,270 + +0.3 1,963,271 - +0.4 1,963,314 + dam HEPCGN_15945 0.13 +0.1 1,963,315 - dam HEPCGN_15945 0.13 +0.1 1,963,355 + dam HEPCGN_15945 0.18 -0.0 1,963,356 - dam HEPCGN_15945 0.18 -0.3 1,963,428 + dam HEPCGN_15945 0.26 -0.2 1,963,429 - dam HEPCGN_15945 0.26 +0.8 1,963,478 + dam HEPCGN_15945 0.32 +3.5 1,963,581 - dam HEPCGN_15945 0.45 +0.6 1,963,688 + dam HEPCGN_15945 0.57 -0.6 1,963,742 + dam HEPCGN_15945 0.64 -0.3 1,963,957 + dam HEPCGN_15945 0.89 +0.8 1,963,960 - dam HEPCGN_15945 0.90 +1.6 1,963,981 + +0.3 1,964,233 - rpe HEPCGN_15950 0.25 +3.1 1,964,328 + rpe HEPCGN_15950 0.39 +0.1 1,964,328 + rpe HEPCGN_15950 0.39 -1.0 1,964,434 + rpe HEPCGN_15950 0.55 +0.8 1,964,553 + rpe HEPCGN_15950 0.72 -1.2 1,964,643 + rpe HEPCGN_15950 0.86 +0.6 1,964,726 + +0.1 1,964,726 + -0.2 1,964,738 - +0.4 1,964,779 + -0.3 1,964,780 - -0.2 1,964,817 + gph HEPCGN_15955 0.11 +0.3 1,964,817 + gph HEPCGN_15955 0.11 +0.5 1,964,817 + gph HEPCGN_15955 0.11 -0.4 1,964,818 - gph HEPCGN_15955 0.11 -0.2 1,964,818 - gph HEPCGN_15955 0.11 +0.2 1,964,821 + gph HEPCGN_15955 0.12 +0.4 1,964,822 - gph HEPCGN_15955 0.12 +0.0 1,964,822 - gph HEPCGN_15955 0.12 -0.1 1,964,871 + gph HEPCGN_15955 0.18 -0.4 1,964,872 - gph HEPCGN_15955 0.18 -0.1 1,964,872 - gph HEPCGN_15955 0.18 +0.2 1,964,872 - gph HEPCGN_15955 0.18 +0.1 1,964,872 - gph HEPCGN_15955 0.18 -0.6 1,964,963 - gph HEPCGN_15955 0.30 +1.0 1,965,007 + gph HEPCGN_15955 0.36 +0.7 1,965,008 - gph HEPCGN_15955 0.36 +0.2 1,965,008 - gph HEPCGN_15955 0.36 -1.0 1,965,008 - gph HEPCGN_15955 0.36 -0.4 1,965,033 - gph HEPCGN_15955 0.40 +0.2 1,965,121 - gph HEPCGN_15955 0.51 +0.1 1,965,121 - gph HEPCGN_15955 0.51 -1.2 1,965,174 + gph HEPCGN_15955 0.58 -1.4 1,965,174 + gph HEPCGN_15955 0.58 +0.4 1,965,188 + gph HEPCGN_15955 0.60 +0.1 1,965,189 - gph HEPCGN_15955 0.60 +0.1 1,965,252 + gph HEPCGN_15955 0.69 -2.4 1,965,253 - gph HEPCGN_15955 0.69 -0.7 1,965,319 + gph HEPCGN_15955 0.77 -1.6 1,965,320 - gph HEPCGN_15955 0.77 +0.5 1,965,372 - gph HEPCGN_15955 0.84 -0.1 1,965,373 + gph HEPCGN_15955 0.84 -0.2 1,965,373 + gph HEPCGN_15955 0.84 +0.2 1,965,374 - gph HEPCGN_15955 0.85 -1.1 1,965,374 - gph HEPCGN_15955 0.85 +1.6 1,965,378 - gph HEPCGN_15955 0.85 -0.5 1,965,378 - gph HEPCGN_15955 0.85 -2.0 1,965,378 - gph HEPCGN_15955 0.85 +0.1 1,965,411 + gph HEPCGN_15955 0.89 -0.3 1,965,424 + -0.4 1,965,426 + -0.3 1,965,426 + -2.3
Or see this region's nucleotide sequence