Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhgF and greB are separated by 96 nucleotides greB and ompR_G63V are separated by 227 nucleotides ompR_G63V and envZ overlap by 4 nucleotides
HEPCGN_15835: yhgF - Protein YhgF, at 1,938,598 to 1,940,919
yhgF
HEPCGN_15840: greB - transcription elongation factor GreB, at 1,941,016 to 1,941,492
greB
HEPCGN_15845: ompR_G63V - two-component system response regulator OmpR, at 1,941,720 to 1,942,439
_G63V
HEPCGN_15850: envZ - two-component system sensor histidine kinase EnvZ, at 1,942,436 to 1,943,788
envZ
Position (kb)
1941
1942
1943 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1940.735 kb on + strand at 1940.735 kb on + strand at 1940.735 kb on + strand at 1940.757 kb on + strand at 1940.823 kb on + strand at 1940.824 kb on - strand at 1940.957 kb on + strand at 1940.957 kb on + strand at 1940.958 kb on - strand at 1940.958 kb on - strand at 1940.958 kb on - strand at 1940.958 kb on - strand at 1940.961 kb on + strand at 1940.963 kb on + strand at 1940.964 kb on - strand at 1941.005 kb on - strand at 1941.018 kb on - strand at 1941.042 kb on + strand at 1941.047 kb on + strand at 1941.144 kb on - strand, within greB at 1941.171 kb on - strand, within greB at 1941.263 kb on + strand, within greB at 1941.302 kb on + strand, within greB at 1941.340 kb on - strand, within greB at 1941.342 kb on + strand, within greB at 1941.344 kb on + strand, within greB at 1941.344 kb on + strand, within greB at 1941.345 kb on - strand, within greB at 1941.351 kb on - strand, within greB at 1941.434 kb on + strand, within greB at 1941.440 kb on - strand, within greB at 1941.535 kb on + strand at 1941.538 kb on - strand at 1941.549 kb on - strand at 1941.578 kb on + strand at 1941.591 kb on + strand at 1941.592 kb on - strand at 1941.592 kb on - strand at 1941.592 kb on - strand at 1941.604 kb on - strand at 1941.604 kb on - strand at 1941.663 kb on - strand at 1941.713 kb on - strand at 1941.732 kb on + strand at 1941.758 kb on + strand at 1941.759 kb on - strand at 1941.786 kb on + strand at 1941.872 kb on + strand, within ompR_G63V at 1941.872 kb on + strand, within ompR_G63V at 1941.873 kb on - strand, within ompR_G63V at 1941.873 kb on - strand, within ompR_G63V at 1941.877 kb on + strand, within ompR_G63V at 1941.892 kb on + strand, within ompR_G63V at 1941.892 kb on + strand, within ompR_G63V at 1941.893 kb on - strand, within ompR_G63V at 1941.959 kb on + strand, within ompR_G63V at 1941.960 kb on - strand, within ompR_G63V at 1941.960 kb on - strand, within ompR_G63V at 1942.299 kb on + strand, within ompR_G63V at 1942.408 kb on - strand at 1942.417 kb on + strand at 1942.430 kb on + strand at 1942.430 kb on + strand at 1942.526 kb on + strand at 1942.527 kb on - strand at 1942.573 kb on + strand, within envZ at 1942.574 kb on - strand, within envZ at 1942.574 kb on - strand, within envZ at 1942.576 kb on + strand, within envZ at 1942.576 kb on + strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.601 kb on - strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.676 kb on + strand, within envZ at 1942.701 kb on - strand, within envZ at 1942.924 kb on + strand, within envZ at 1942.924 kb on + strand, within envZ at 1942.925 kb on - strand, within envZ at 1942.937 kb on - strand, within envZ at 1943.036 kb on - strand, within envZ at 1943.057 kb on + strand, within envZ at 1943.116 kb on + strand, within envZ at 1943.117 kb on - strand, within envZ at 1943.174 kb on - strand, within envZ at 1943.229 kb on - strand, within envZ at 1943.249 kb on - strand, within envZ at 1943.295 kb on - strand, within envZ at 1943.379 kb on - strand, within envZ
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 1,940,735 + +0.6 1,940,735 + -0.9 1,940,735 + +0.2 1,940,757 + -0.8 1,940,823 + -0.7 1,940,824 - +0.3 1,940,957 + +1.5 1,940,957 + -0.1 1,940,958 - -0.3 1,940,958 - -2.2 1,940,958 - -0.4 1,940,958 - -0.4 1,940,961 + -0.3 1,940,963 + +3.1 1,940,964 - -0.0 1,941,005 - -0.6 1,941,018 - -0.4 1,941,042 + -0.0 1,941,047 + -0.3 1,941,144 - greB HEPCGN_15840 0.27 +0.0 1,941,171 - greB HEPCGN_15840 0.32 +0.5 1,941,263 + greB HEPCGN_15840 0.52 -0.0 1,941,302 + greB HEPCGN_15840 0.60 +0.2 1,941,340 - greB HEPCGN_15840 0.68 +0.2 1,941,342 + greB HEPCGN_15840 0.68 -0.3 1,941,344 + greB HEPCGN_15840 0.69 -0.3 1,941,344 + greB HEPCGN_15840 0.69 +0.4 1,941,345 - greB HEPCGN_15840 0.69 -1.2 1,941,351 - greB HEPCGN_15840 0.70 +0.8 1,941,434 + greB HEPCGN_15840 0.88 -0.7 1,941,440 - greB HEPCGN_15840 0.89 +0.1 1,941,535 + +0.7 1,941,538 - -0.1 1,941,549 - +0.4 1,941,578 + -0.4 1,941,591 + -0.6 1,941,592 - +0.2 1,941,592 - -0.4 1,941,592 - +1.3 1,941,604 - -0.4 1,941,604 - -0.0 1,941,663 - -0.6 1,941,713 - -0.8 1,941,732 + +0.0 1,941,758 + -0.2 1,941,759 - +0.6 1,941,786 + -1.4 1,941,872 + ompR_G63V HEPCGN_15845 0.21 -0.6 1,941,872 + ompR_G63V HEPCGN_15845 0.21 +1.5 1,941,873 - ompR_G63V HEPCGN_15845 0.21 -1.7 1,941,873 - ompR_G63V HEPCGN_15845 0.21 -0.7 1,941,877 + ompR_G63V HEPCGN_15845 0.22 -0.8 1,941,892 + ompR_G63V HEPCGN_15845 0.24 -1.1 1,941,892 + ompR_G63V HEPCGN_15845 0.24 +0.8 1,941,893 - ompR_G63V HEPCGN_15845 0.24 -1.2 1,941,959 + ompR_G63V HEPCGN_15845 0.33 -0.6 1,941,960 - ompR_G63V HEPCGN_15845 0.33 -1.2 1,941,960 - ompR_G63V HEPCGN_15845 0.33 -0.2 1,942,299 + ompR_G63V HEPCGN_15845 0.80 -1.8 1,942,408 - +1.0 1,942,417 + -0.7 1,942,430 + +0.1 1,942,430 + +0.3 1,942,526 + -1.9 1,942,527 - +0.2 1,942,573 + envZ HEPCGN_15850 0.10 +0.2 1,942,574 - envZ HEPCGN_15850 0.10 -0.0 1,942,574 - envZ HEPCGN_15850 0.10 +0.1 1,942,576 + envZ HEPCGN_15850 0.10 -1.0 1,942,576 + envZ HEPCGN_15850 0.10 -0.2 1,942,601 - envZ HEPCGN_15850 0.12 -0.3 1,942,601 - envZ HEPCGN_15850 0.12 -0.2 1,942,601 - envZ HEPCGN_15850 0.12 +0.1 1,942,601 - envZ HEPCGN_15850 0.12 -2.7 1,942,676 + envZ HEPCGN_15850 0.18 -0.5 1,942,676 + envZ HEPCGN_15850 0.18 -0.0 1,942,676 + envZ HEPCGN_15850 0.18 -1.3 1,942,701 - envZ HEPCGN_15850 0.20 +0.3 1,942,924 + envZ HEPCGN_15850 0.36 -0.9 1,942,924 + envZ HEPCGN_15850 0.36 -0.4 1,942,925 - envZ HEPCGN_15850 0.36 +0.5 1,942,937 - envZ HEPCGN_15850 0.37 -1.4 1,943,036 - envZ HEPCGN_15850 0.44 -0.1 1,943,057 + envZ HEPCGN_15850 0.46 +1.9 1,943,116 + envZ HEPCGN_15850 0.50 -0.8 1,943,117 - envZ HEPCGN_15850 0.50 -0.6 1,943,174 - envZ HEPCGN_15850 0.55 +1.2 1,943,229 - envZ HEPCGN_15850 0.59 +0.1 1,943,249 - envZ HEPCGN_15850 0.60 +0.2 1,943,295 - envZ HEPCGN_15850 0.63 +0.9 1,943,379 - envZ HEPCGN_15850 0.70 +0.1
Or see this region's nucleotide sequence