Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiB and ubiJ overlap by 4 nucleotides ubiJ and ubiE are separated by 13 nucleotides ubiE and rmuC are separated by 94 nucleotides
HEPCGN_12920: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 1,333,382 to 1,335,022
ubiB
HEPCGN_12930: ubiJ - ubiquinone biosynthesis protein UbiJ, at 1,335,019 to 1,335,624
ubiJ
HEPCGN_12935: ubiE - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 1,335,638 to 1,336,393
ubiE
HEPCGN_12940: rmuC - DNA recombination protein RmuC, at 1,336,488 to 1,337,915
rmuC
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-2
-1
0
1 at 1335.449 kb on - strand, within ubiJ at 1335.449 kb on - strand, within ubiJ at 1335.503 kb on - strand, within ubiJ at 1335.616 kb on - strand at 1335.647 kb on - strand at 1335.647 kb on - strand at 1335.647 kb on - strand at 1335.647 kb on - strand at 1335.774 kb on + strand, within ubiE at 1335.781 kb on - strand, within ubiE at 1335.838 kb on - strand, within ubiE at 1336.109 kb on - strand, within ubiE at 1336.471 kb on + strand at 1336.472 kb on - strand at 1336.505 kb on + strand at 1336.505 kb on + strand at 1336.506 kb on - strand at 1336.525 kb on + strand at 1336.567 kb on + strand at 1336.610 kb on + strand at 1336.623 kb on - strand at 1336.709 kb on - strand, within rmuC at 1336.712 kb on + strand, within rmuC at 1336.713 kb on - strand, within rmuC at 1336.713 kb on - strand, within rmuC at 1336.780 kb on + strand, within rmuC at 1336.780 kb on + strand, within rmuC at 1336.780 kb on + strand, within rmuC at 1336.898 kb on + strand, within rmuC at 1336.899 kb on - strand, within rmuC at 1336.973 kb on + strand, within rmuC at 1337.045 kb on + strand, within rmuC at 1337.052 kb on - strand, within rmuC at 1337.095 kb on + strand, within rmuC at 1337.096 kb on - strand, within rmuC at 1337.117 kb on - strand, within rmuC at 1337.243 kb on - strand, within rmuC at 1337.289 kb on + strand, within rmuC at 1337.289 kb on + strand, within rmuC at 1337.289 kb on + strand, within rmuC at 1337.292 kb on + strand, within rmuC at 1337.318 kb on + strand, within rmuC at 1337.318 kb on + strand, within rmuC
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 1,335,449 - ubiJ HEPCGN_12930 0.71 -0.0 1,335,449 - ubiJ HEPCGN_12930 0.71 -2.6 1,335,503 - ubiJ HEPCGN_12930 0.80 -0.3 1,335,616 - -0.2 1,335,647 - -1.0 1,335,647 - +0.2 1,335,647 - -0.2 1,335,647 - -0.2 1,335,774 + ubiE HEPCGN_12935 0.18 -0.1 1,335,781 - ubiE HEPCGN_12935 0.19 +0.9 1,335,838 - ubiE HEPCGN_12935 0.26 -0.4 1,336,109 - ubiE HEPCGN_12935 0.62 +0.4 1,336,471 + +0.2 1,336,472 - +0.5 1,336,505 + +0.7 1,336,505 + -1.0 1,336,506 - -0.6 1,336,525 + -0.2 1,336,567 + -0.0 1,336,610 + +0.2 1,336,623 - -0.4 1,336,709 - rmuC HEPCGN_12940 0.15 -0.2 1,336,712 + rmuC HEPCGN_12940 0.16 +0.7 1,336,713 - rmuC HEPCGN_12940 0.16 -0.4 1,336,713 - rmuC HEPCGN_12940 0.16 +0.1 1,336,780 + rmuC HEPCGN_12940 0.20 +0.0 1,336,780 + rmuC HEPCGN_12940 0.20 -0.7 1,336,780 + rmuC HEPCGN_12940 0.20 +0.1 1,336,898 + rmuC HEPCGN_12940 0.29 -0.5 1,336,899 - rmuC HEPCGN_12940 0.29 +0.2 1,336,973 + rmuC HEPCGN_12940 0.34 -0.5 1,337,045 + rmuC HEPCGN_12940 0.39 +0.6 1,337,052 - rmuC HEPCGN_12940 0.39 +0.3 1,337,095 + rmuC HEPCGN_12940 0.43 -1.3 1,337,096 - rmuC HEPCGN_12940 0.43 -1.2 1,337,117 - rmuC HEPCGN_12940 0.44 +0.7 1,337,243 - rmuC HEPCGN_12940 0.53 -0.1 1,337,289 + rmuC HEPCGN_12940 0.56 +0.1 1,337,289 + rmuC HEPCGN_12940 0.56 +0.1 1,337,289 + rmuC HEPCGN_12940 0.56 +0.2 1,337,292 + rmuC HEPCGN_12940 0.56 +0.9 1,337,318 + rmuC HEPCGN_12940 0.58 +0.6 1,337,318 + rmuC HEPCGN_12940 0.58 -0.6
Or see this region's nucleotide sequence