Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mraZ and cra are separated by 601 nucleotides cra and fruL are separated by 81 nucleotides fruL and ilvN are separated by 11 nucleotides ilvN and ilvI are separated by 2 nucleotides
HEPCGN_08660: mraZ - division/cell wall cluster transcriptional repressor MraZ, at 472,304 to 472,762
mraZ
HEPCGN_08665: cra - catabolite repressor/activator, at 473,364 to 474,368
cra
HEPCGN_08670: fruL - Putative fruR/shl operon leader peptide, at 474,450 to 474,536
fruL
HEPCGN_08675: ilvN - acetolactate synthase small subunit, at 474,548 to 475,039
ilvN
HEPCGN_08680: ilvI - acetolactate synthase 3 large subunit, at 475,042 to 476,766
ilvI
Position (kb) 473
474
475 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 472.530 kb on - strand, within mraZ at 472.530 kb on - strand, within mraZ at 472.939 kb on + strand at 472.945 kb on + strand at 472.945 kb on + strand at 472.946 kb on - strand at 472.946 kb on - strand at 472.946 kb on - strand at 472.951 kb on + strand at 472.997 kb on - strand at 473.015 kb on - strand at 473.047 kb on - strand at 473.053 kb on + strand at 473.053 kb on + strand at 473.053 kb on + strand at 473.054 kb on - strand at 473.172 kb on - strand at 473.200 kb on + strand at 473.200 kb on + strand at 473.204 kb on + strand at 473.204 kb on + strand at 473.228 kb on + strand at 473.233 kb on - strand at 473.255 kb on + strand at 473.256 kb on - strand at 473.261 kb on + strand at 473.265 kb on - strand at 473.280 kb on + strand at 473.349 kb on - strand at 473.400 kb on - strand at 473.400 kb on - strand at 473.406 kb on - strand at 473.523 kb on + strand, within cra at 473.524 kb on - strand, within cra at 473.596 kb on - strand, within cra at 473.686 kb on - strand, within cra at 473.818 kb on + strand, within cra at 473.818 kb on + strand, within cra at 473.844 kb on - strand, within cra at 473.847 kb on + strand, within cra at 474.124 kb on + strand, within cra at 474.124 kb on + strand, within cra at 474.125 kb on - strand, within cra at 474.125 kb on - strand, within cra at 474.129 kb on - strand, within cra at 474.143 kb on - strand, within cra at 474.179 kb on + strand, within cra at 474.229 kb on + strand, within cra at 474.230 kb on - strand, within cra at 474.313 kb on + strand at 474.314 kb on - strand at 474.314 kb on - strand at 474.314 kb on - strand at 474.314 kb on - strand at 474.314 kb on - strand at 474.384 kb on - strand at 474.661 kb on + strand, within ilvN at 474.661 kb on + strand, within ilvN at 474.682 kb on - strand, within ilvN at 475.026 kb on - strand at 475.026 kb on - strand at 475.029 kb on - strand at 475.039 kb on + strand at 475.039 kb on + strand at 475.106 kb on + strand at 475.155 kb on + strand at 475.275 kb on + strand, within ilvI at 475.277 kb on + strand, within ilvI at 475.277 kb on + strand, within ilvI at 475.277 kb on + strand, within ilvI at 475.277 kb on + strand, within ilvI at 475.277 kb on + strand, within ilvI at 475.278 kb on - strand, within ilvI at 475.278 kb on - strand, within ilvI at 475.278 kb on - strand, within ilvI at 475.278 kb on - strand, within ilvI at 475.301 kb on + strand, within ilvI at 475.301 kb on + strand, within ilvI
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 472,530 - mraZ HEPCGN_08660 0.49 -0.6 472,530 - mraZ HEPCGN_08660 0.49 +0.8 472,939 + +1.8 472,945 + -2.4 472,945 + -0.5 472,946 - +0.4 472,946 - -0.4 472,946 - -0.4 472,951 + -0.4 472,997 - +0.5 473,015 - +0.3 473,047 - -0.6 473,053 + +0.9 473,053 + +0.1 473,053 + -0.5 473,054 - -1.1 473,172 - +0.8 473,200 + +0.1 473,200 + +1.6 473,204 + +0.5 473,204 + -0.0 473,228 + +0.6 473,233 - -0.5 473,255 + +0.5 473,256 - +0.1 473,261 + +0.8 473,265 - +0.3 473,280 + +1.2 473,349 - -0.2 473,400 - +1.1 473,400 - -2.8 473,406 - -2.8 473,523 + cra HEPCGN_08665 0.16 +0.2 473,524 - cra HEPCGN_08665 0.16 -2.2 473,596 - cra HEPCGN_08665 0.23 +0.1 473,686 - cra HEPCGN_08665 0.32 -0.3 473,818 + cra HEPCGN_08665 0.45 -3.5 473,818 + cra HEPCGN_08665 0.45 -3.0 473,844 - cra HEPCGN_08665 0.48 +0.4 473,847 + cra HEPCGN_08665 0.48 -1.4 474,124 + cra HEPCGN_08665 0.76 -1.7 474,124 + cra HEPCGN_08665 0.76 -1.7 474,125 - cra HEPCGN_08665 0.76 -1.4 474,125 - cra HEPCGN_08665 0.76 -3.2 474,129 - cra HEPCGN_08665 0.76 -0.3 474,143 - cra HEPCGN_08665 0.78 -2.4 474,179 + cra HEPCGN_08665 0.81 -2.0 474,229 + cra HEPCGN_08665 0.86 -3.3 474,230 - cra HEPCGN_08665 0.86 -3.1 474,313 + -0.8 474,314 - -0.1 474,314 - -1.4 474,314 - -0.0 474,314 - +0.5 474,314 - +1.0 474,384 - -0.6 474,661 + ilvN HEPCGN_08675 0.23 +1.3 474,661 + ilvN HEPCGN_08675 0.23 -0.1 474,682 - ilvN HEPCGN_08675 0.27 +0.1 475,026 - +0.6 475,026 - +0.4 475,029 - -0.4 475,039 + +0.8 475,039 + +0.7 475,106 + +1.0 475,155 + -0.9 475,275 + ilvI HEPCGN_08680 0.14 -0.4 475,277 + ilvI HEPCGN_08680 0.14 +0.6 475,277 + ilvI HEPCGN_08680 0.14 -0.6 475,277 + ilvI HEPCGN_08680 0.14 +0.9 475,277 + ilvI HEPCGN_08680 0.14 -0.5 475,277 + ilvI HEPCGN_08680 0.14 +0.2 475,278 - ilvI HEPCGN_08680 0.14 +0.6 475,278 - ilvI HEPCGN_08680 0.14 -0.2 475,278 - ilvI HEPCGN_08680 0.14 -0.7 475,278 - ilvI HEPCGN_08680 0.14 +0.4 475,301 + ilvI HEPCGN_08680 0.15 -1.3 475,301 + ilvI HEPCGN_08680 0.15 -0.7
Or see this region's nucleotide sequence