Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acnA and ribA are separated by 63 nucleotides ribA and pgpB are separated by 169 nucleotides pgpB and lapA are separated by 148 nucleotides lapA and lapB are separated by 6 nucleotides
HEPCGN_25625: acnA - aconitate hydratase AcnA, at 3,859,847 to 3,862,522
acnA
HEPCGN_25630: ribA - GTP cyclohydrolase-2, at 3,862,586 to 3,863,176
ribA
HEPCGN_25635: pgpB - phosphatidylglycerophosphatase B, at 3,863,346 to 3,864,110
pgpB
HEPCGN_25640: lapA - lipopolysaccharide assembly protein LapA, at 3,864,259 to 3,864,567
lapA
HEPCGN_25645: lapB - lipopolysaccharide assembly protein LapB, at 3,864,574 to 3,865,743
lapB
Position (kb)
3863
3864
3865 Strain fitness (log2 ratio)
-2
-1
0
1 at 3862.348 kb on + strand at 3862.349 kb on - strand at 3862.400 kb on - strand at 3862.472 kb on + strand at 3862.497 kb on - strand at 3862.511 kb on - strand at 3862.587 kb on + strand at 3863.205 kb on - strand at 3863.213 kb on - strand at 3863.313 kb on + strand at 3863.313 kb on + strand at 3863.387 kb on - strand at 3863.397 kb on - strand at 3863.561 kb on + strand, within pgpB at 3863.578 kb on + strand, within pgpB at 3863.690 kb on + strand, within pgpB at 3863.691 kb on - strand, within pgpB at 3863.909 kb on + strand, within pgpB at 3863.910 kb on - strand, within pgpB at 3863.986 kb on + strand, within pgpB at 3863.987 kb on - strand, within pgpB at 3863.991 kb on - strand, within pgpB at 3863.991 kb on - strand, within pgpB at 3863.991 kb on - strand, within pgpB at 3864.055 kb on + strand at 3864.055 kb on + strand at 3864.196 kb on + strand at 3864.317 kb on + strand, within lapA at 3864.348 kb on + strand, within lapA at 3864.491 kb on + strand, within lapA at 3864.491 kb on + strand, within lapA
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 3,862,348 + +0.6 3,862,349 - -0.1 3,862,400 - +1.7 3,862,472 + +0.7 3,862,497 - -1.5 3,862,511 - -0.5 3,862,587 + -0.3 3,863,205 - -1.4 3,863,213 - +0.1 3,863,313 + +0.7 3,863,313 + +0.2 3,863,387 - +0.8 3,863,397 - +0.5 3,863,561 + pgpB HEPCGN_25635 0.28 -0.4 3,863,578 + pgpB HEPCGN_25635 0.30 +1.0 3,863,690 + pgpB HEPCGN_25635 0.45 +1.2 3,863,691 - pgpB HEPCGN_25635 0.45 +0.1 3,863,909 + pgpB HEPCGN_25635 0.74 -2.5 3,863,910 - pgpB HEPCGN_25635 0.74 -0.6 3,863,986 + pgpB HEPCGN_25635 0.84 +0.1 3,863,987 - pgpB HEPCGN_25635 0.84 -1.9 3,863,991 - pgpB HEPCGN_25635 0.84 -0.1 3,863,991 - pgpB HEPCGN_25635 0.84 +0.3 3,863,991 - pgpB HEPCGN_25635 0.84 -0.4 3,864,055 + -1.1 3,864,055 + -1.3 3,864,196 + +0.1 3,864,317 + lapA HEPCGN_25640 0.19 -1.8 3,864,348 + lapA HEPCGN_25640 0.29 -0.4 3,864,491 + lapA HEPCGN_25640 0.75 +0.2 3,864,491 + lapA HEPCGN_25640 0.75 -1.3
Or see this region's nucleotide sequence