Experiment: K25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdxA and rsmA overlap by 4 nucleotides rsmA and apaG are separated by 2 nucleotides apaG and apaH are separated by 6 nucleotides
HEPCGN_08820: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase PdxA, at 509,000 to 509,989
pdxA
HEPCGN_08825: rsmA - 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA, at 509,986 to 510,807
rsmA
HEPCGN_08830: apaG - Co2+/Mg2+ efflux protein ApaG, at 510,810 to 511,187
apaG
HEPCGN_08835: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 511,194 to 512,042
apaH
Position (kb) 510
511
512 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 509.818 kb on - strand, within pdxA at 509.942 kb on - strand at 509.977 kb on + strand at 510.014 kb on + strand at 510.332 kb on - strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.333 kb on + strand, within rsmA at 510.334 kb on - strand, within rsmA at 510.334 kb on - strand, within rsmA at 510.367 kb on - strand, within rsmA at 510.371 kb on - strand, within rsmA at 510.371 kb on - strand, within rsmA at 510.399 kb on + strand, within rsmA at 510.399 kb on + strand, within rsmA at 510.454 kb on + strand, within rsmA at 510.481 kb on + strand, within rsmA at 510.663 kb on + strand, within rsmA at 510.773 kb on - strand at 510.853 kb on - strand, within apaG at 510.901 kb on - strand, within apaG at 510.913 kb on + strand, within apaG at 511.028 kb on + strand, within apaG at 511.053 kb on + strand, within apaG at 511.185 kb on + strand at 511.203 kb on + strand at 511.203 kb on + strand at 511.203 kb on + strand at 511.204 kb on - strand at 511.204 kb on - strand at 511.204 kb on - strand at 511.208 kb on + strand at 511.230 kb on + strand at 511.231 kb on - strand at 511.256 kb on + strand at 511.261 kb on + strand at 511.269 kb on - strand at 511.283 kb on + strand, within apaH at 511.369 kb on + strand, within apaH at 511.370 kb on - strand, within apaH at 511.370 kb on - strand, within apaH at 511.434 kb on - strand, within apaH at 511.491 kb on - strand, within apaH at 511.609 kb on + strand, within apaH at 511.610 kb on - strand, within apaH at 511.610 kb on - strand, within apaH at 511.635 kb on + strand, within apaH at 511.659 kb on + strand, within apaH at 511.660 kb on - strand, within apaH at 511.772 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.776 kb on + strand, within apaH at 511.946 kb on + strand, within apaH at 511.947 kb on - strand, within apaH at 511.973 kb on + strand at 511.974 kb on - strand at 511.981 kb on - strand at 512.164 kb on - strand at 512.164 kb on - strand at 512.178 kb on - strand at 512.178 kb on - strand at 512.183 kb on + strand at 512.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K25 remove 509,818 - pdxA HEPCGN_08820 0.83 +0.6 509,942 - +2.4 509,977 + -0.3 510,014 + -1.5 510,332 - rsmA HEPCGN_08825 0.42 -0.8 510,333 + rsmA HEPCGN_08825 0.42 +0.4 510,333 + rsmA HEPCGN_08825 0.42 +0.6 510,333 + rsmA HEPCGN_08825 0.42 +1.2 510,333 + rsmA HEPCGN_08825 0.42 +1.4 510,334 - rsmA HEPCGN_08825 0.42 +0.8 510,334 - rsmA HEPCGN_08825 0.42 +1.2 510,367 - rsmA HEPCGN_08825 0.46 -0.3 510,371 - rsmA HEPCGN_08825 0.47 -0.4 510,371 - rsmA HEPCGN_08825 0.47 +0.2 510,399 + rsmA HEPCGN_08825 0.50 -0.6 510,399 + rsmA HEPCGN_08825 0.50 -0.3 510,454 + rsmA HEPCGN_08825 0.57 -0.2 510,481 + rsmA HEPCGN_08825 0.60 -1.3 510,663 + rsmA HEPCGN_08825 0.82 +0.8 510,773 - +2.5 510,853 - apaG HEPCGN_08830 0.11 +0.7 510,901 - apaG HEPCGN_08830 0.24 -1.3 510,913 + apaG HEPCGN_08830 0.27 +1.3 511,028 + apaG HEPCGN_08830 0.58 +1.3 511,053 + apaG HEPCGN_08830 0.64 +0.7 511,185 + -1.2 511,203 + -2.0 511,203 + -1.4 511,203 + -1.4 511,204 - -1.6 511,204 - -1.5 511,204 - +0.3 511,208 + -1.0 511,230 + +0.4 511,231 - +0.4 511,256 + -1.2 511,261 + -2.0 511,269 - -0.3 511,283 + apaH HEPCGN_08835 0.10 -0.4 511,369 + apaH HEPCGN_08835 0.21 +0.2 511,370 - apaH HEPCGN_08835 0.21 -3.1 511,370 - apaH HEPCGN_08835 0.21 +0.7 511,434 - apaH HEPCGN_08835 0.28 -1.9 511,491 - apaH HEPCGN_08835 0.35 -0.5 511,609 + apaH HEPCGN_08835 0.49 +2.6 511,610 - apaH HEPCGN_08835 0.49 -1.0 511,610 - apaH HEPCGN_08835 0.49 -3.6 511,635 + apaH HEPCGN_08835 0.52 -2.8 511,659 + apaH HEPCGN_08835 0.55 -0.1 511,660 - apaH HEPCGN_08835 0.55 -1.2 511,772 + apaH HEPCGN_08835 0.68 -0.8 511,776 + apaH HEPCGN_08835 0.69 -2.4 511,776 + apaH HEPCGN_08835 0.69 -0.5 511,946 + apaH HEPCGN_08835 0.89 +2.4 511,947 - apaH HEPCGN_08835 0.89 -2.5 511,973 + +0.2 511,974 - -1.3 511,981 - -0.2 512,164 - -0.5 512,164 - +0.3 512,178 - -0.3 512,178 - +0.4 512,183 + +1.0 512,183 + +0.1
Or see this region's nucleotide sequence