Strain Fitness in Escherichia coli ECOR38 around HEPCGN_21120

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttmcA and ypfH are separated by 73 nucleotidesypfH and ypfN are separated by 80 nucleotidesypfN and dapE are separated by 27 nucleotides HEPCGN_21110: tmcA - tRNA cytosine(34) acetyltransferase TmcA, at 2,973,004 to 2,975,019 tmcA HEPCGN_21115: ypfH - esterase, at 2,975,093 to 2,975,791 ypfH HEPCGN_21120: ypfN - UPF0370 protein YpfN, at 2,975,872 to 2,976,072 ypfN HEPCGN_21125: dapE - succinyl-diaminopimelate desuccinylase, at 2,976,100 to 2,977,227 dapE Position (kb) 2975 2976 2977Strain fitness (log2 ratio) -1 0 1at 2974.877 kb on + strandat 2974.878 kb on - strandat 2974.878 kb on - strandat 2974.878 kb on - strandat 2974.913 kb on + strandat 2974.914 kb on - strandat 2975.128 kb on + strandat 2975.197 kb on - strand, within ypfHat 2975.200 kb on + strand, within ypfHat 2975.201 kb on - strand, within ypfHat 2975.303 kb on - strand, within ypfHat 2975.305 kb on + strand, within ypfHat 2975.314 kb on + strand, within ypfHat 2975.315 kb on - strand, within ypfHat 2975.438 kb on - strand, within ypfHat 2975.456 kb on - strand, within ypfHat 2975.614 kb on - strand, within ypfHat 2975.713 kb on + strand, within ypfHat 2975.713 kb on + strand, within ypfHat 2975.729 kb on + strandat 2975.790 kb on - strandat 2975.792 kb on + strandat 2975.795 kb on + strandat 2975.812 kb on + strandat 2975.813 kb on - strandat 2975.869 kb on - strandat 2975.869 kb on - strandat 2975.869 kb on - strandat 2975.869 kb on - strandat 2975.874 kb on - strandat 2976.022 kb on + strand, within ypfNat 2976.057 kb on + strandat 2976.076 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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2,974,877 + -1.7
2,974,878 - -0.2
2,974,878 - -0.2
2,974,878 - -1.0
2,974,913 + +0.2
2,974,914 - -0.0
2,975,128 + -0.2
2,975,197 - ypfH HEPCGN_21115 0.15 +1.2
2,975,200 + ypfH HEPCGN_21115 0.15 +0.3
2,975,201 - ypfH HEPCGN_21115 0.15 +0.4
2,975,303 - ypfH HEPCGN_21115 0.30 +0.3
2,975,305 + ypfH HEPCGN_21115 0.30 +0.2
2,975,314 + ypfH HEPCGN_21115 0.32 +1.0
2,975,315 - ypfH HEPCGN_21115 0.32 +0.5
2,975,438 - ypfH HEPCGN_21115 0.49 -0.9
2,975,456 - ypfH HEPCGN_21115 0.52 +0.2
2,975,614 - ypfH HEPCGN_21115 0.75 -1.0
2,975,713 + ypfH HEPCGN_21115 0.89 +0.2
2,975,713 + ypfH HEPCGN_21115 0.89 +0.8
2,975,729 + +0.6
2,975,790 - -0.1
2,975,792 + -0.3
2,975,795 + +0.2
2,975,812 + +0.0
2,975,813 - +0.3
2,975,869 - -0.4
2,975,869 - -0.6
2,975,869 - +0.4
2,975,869 - -0.1
2,975,874 - +0.4
2,976,022 + ypfN HEPCGN_21120 0.75 -1.2
2,976,057 + -0.0
2,976,076 + -0.2

Or see this region's nucleotide sequence