Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdtA and HEPCGN_02900 are separated by 278 nucleotides HEPCGN_02900 and HEPCGN_02905 are separated by 162 nucleotides HEPCGN_02905 and HEPCGN_02915 overlap by 35 nucleotides HEPCGN_02915 and HEPCGN_02925 are separated by 271 nucleotides HEPCGN_02925 and ibsA are separated by 272 nucleotides
HEPCGN_02890: mdtA - multidrug efflux RND transporter subunit MdtA, at 490,241 to 491,488
mdtA
HEPCGN_02900: HEPCGN_02900 - Type I toxin-antitoxin system Ibs family toxin, at 491,767 to 491,823
_02900
HEPCGN_02905: HEPCGN_02905 - hypothetical protein, at 491,986 to 492,132
_02905
HEPCGN_02915: HEPCGN_02915 - Type I toxin-antitoxin system Ibs family toxin, at 492,098 to 492,151
_02915
HEPCGN_02925: HEPCGN_02925 - Type I toxin-antitoxin system Ibs family toxin, at 492,423 to 492,479
_02925
HEPCGN_02935: ibsA - Small toxic protein IbsA, at 492,752 to 492,811
ibsA
Position (kb)
491
492 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 490.775 kb on - strand, within mdtA at 491.109 kb on - strand, within mdtA at 491.231 kb on - strand, within mdtA at 491.266 kb on - strand, within mdtA at 491.453 kb on + strand at 491.454 kb on - strand at 491.473 kb on + strand at 491.492 kb on + strand at 491.528 kb on + strand at 491.529 kb on - strand at 491.531 kb on + strand at 491.531 kb on + strand at 491.531 kb on + strand at 491.531 kb on + strand at 491.531 kb on + strand at 491.532 kb on - strand at 491.570 kb on - strand at 491.671 kb on - strand at 491.806 kb on - strand, within HEPCGN_02900 at 491.822 kb on - strand at 491.822 kb on - strand at 491.971 kb on + strand at 492.132 kb on + strand at 492.150 kb on - strand at 492.198 kb on + strand at 492.454 kb on + strand, within HEPCGN_02925 at 492.484 kb on - strand at 492.565 kb on - strand at 492.771 kb on + strand, within ibsA at 492.781 kb on - strand, within ibsA at 492.781 kb on - strand, within ibsA at 492.809 kb on + strand at 492.810 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 490,775 - mdtA HEPCGN_02890 0.43 -0.4 491,109 - mdtA HEPCGN_02890 0.70 +0.3 491,231 - mdtA HEPCGN_02890 0.79 +0.3 491,266 - mdtA HEPCGN_02890 0.82 -0.6 491,453 + +0.3 491,454 - +1.3 491,473 + +1.2 491,492 + +0.2 491,528 + +0.8 491,529 - -0.4 491,531 + -0.3 491,531 + +0.6 491,531 + -1.5 491,531 + -0.4 491,531 + -0.1 491,532 - -1.1 491,570 - -1.3 491,671 - -0.9 491,806 - HEPCGN_02900 0.68 -3.1 491,822 - -0.2 491,822 - +0.9 491,971 + -1.0 492,132 + -0.0 492,150 - +0.1 492,198 + -0.6 492,454 + HEPCGN_02925 0.54 -1.7 492,484 - +0.3 492,565 - -2.4 492,771 + ibsA HEPCGN_02935 0.32 -0.9 492,781 - ibsA HEPCGN_02935 0.48 -1.4 492,781 - ibsA HEPCGN_02935 0.48 -0.6 492,809 + +2.5 492,810 - +1.4
Or see this region's nucleotide sequence