Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kch and yciI are separated by 299 nucleotides yciI and tonB are separated by 223 nucleotides tonB and yciA are separated by 39 nucleotides yciA and yciB are separated by 104 nucleotides
HEPCGN_25130: kch - voltage-gated potassium channel protein, at 3,781,060 to 3,782,313
kch
HEPCGN_25135: yciI - YciI family protein, at 3,782,613 to 3,782,909
yciI
HEPCGN_25140: tonB - TonB system transport protein TonB, at 3,783,133 to 3,783,849
tonB
HEPCGN_25145: yciA - acyl-CoA thioester hydrolase YciA, at 3,783,889 to 3,784,287
yciA
HEPCGN_25150: yciB - septation protein A, at 3,784,392 to 3,784,931
yciB
Position (kb)
3783
3784 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3782.298 kb on + strand at 3782.341 kb on + strand at 3782.372 kb on + strand at 3782.401 kb on + strand at 3782.404 kb on + strand at 3782.404 kb on + strand at 3782.491 kb on - strand at 3782.491 kb on - strand at 3782.501 kb on - strand at 3782.512 kb on + strand at 3782.512 kb on + strand at 3782.512 kb on + strand at 3782.513 kb on - strand at 3782.515 kb on + strand at 3782.549 kb on + strand at 3782.550 kb on - strand at 3782.611 kb on - strand at 3782.627 kb on + strand at 3782.667 kb on + strand, within yciI at 3782.820 kb on + strand, within yciI at 3782.820 kb on + strand, within yciI at 3782.821 kb on - strand, within yciI at 3782.821 kb on - strand, within yciI at 3782.826 kb on + strand, within yciI at 3782.893 kb on + strand at 3782.897 kb on + strand at 3782.898 kb on - strand at 3782.938 kb on + strand at 3782.943 kb on + strand at 3782.943 kb on + strand at 3783.123 kb on + strand at 3783.218 kb on - strand, within tonB at 3783.245 kb on + strand, within tonB at 3783.245 kb on + strand, within tonB at 3783.245 kb on + strand, within tonB at 3783.338 kb on + strand, within tonB at 3783.596 kb on + strand, within tonB at 3783.616 kb on + strand, within tonB at 3783.854 kb on - strand at 3783.890 kb on + strand at 3783.890 kb on + strand at 3783.902 kb on - strand at 3783.902 kb on - strand at 3783.938 kb on + strand, within yciA at 3783.939 kb on - strand, within yciA at 3783.939 kb on - strand, within yciA at 3783.942 kb on + strand, within yciA at 3783.943 kb on - strand, within yciA at 3783.943 kb on - strand, within yciA at 3783.943 kb on - strand, within yciA at 3783.965 kb on + strand, within yciA at 3783.966 kb on - strand, within yciA at 3784.014 kb on + strand, within yciA at 3784.017 kb on + strand, within yciA at 3784.018 kb on - strand, within yciA at 3784.094 kb on - strand, within yciA at 3784.247 kb on - strand, within yciA at 3784.272 kb on + strand at 3784.282 kb on - strand at 3784.282 kb on - strand at 3784.290 kb on + strand at 3784.290 kb on + strand at 3784.291 kb on - strand at 3784.291 kb on - strand at 3784.302 kb on - strand at 3784.302 kb on - strand at 3784.328 kb on - strand at 3784.360 kb on - strand at 3784.401 kb on - strand at 3784.428 kb on - strand at 3784.430 kb on - strand at 3784.525 kb on + strand, within yciB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 3,782,298 + +1.0 3,782,341 + +1.2 3,782,372 + -0.1 3,782,401 + +0.1 3,782,404 + -0.4 3,782,404 + -0.4 3,782,491 - +0.1 3,782,491 - +0.3 3,782,501 - +0.6 3,782,512 + +0.3 3,782,512 + -2.2 3,782,512 + +0.9 3,782,513 - +0.1 3,782,515 + +1.0 3,782,549 + +0.9 3,782,550 - +0.7 3,782,611 - +0.1 3,782,627 + -2.0 3,782,667 + yciI HEPCGN_25135 0.18 +2.8 3,782,820 + yciI HEPCGN_25135 0.70 +0.5 3,782,820 + yciI HEPCGN_25135 0.70 +1.2 3,782,821 - yciI HEPCGN_25135 0.70 -0.0 3,782,821 - yciI HEPCGN_25135 0.70 -1.3 3,782,826 + yciI HEPCGN_25135 0.72 -0.1 3,782,893 + -0.9 3,782,897 + -0.8 3,782,898 - -0.4 3,782,938 + +0.6 3,782,943 + -1.3 3,782,943 + +1.3 3,783,123 + +0.7 3,783,218 - tonB HEPCGN_25140 0.12 +1.1 3,783,245 + tonB HEPCGN_25140 0.16 -1.5 3,783,245 + tonB HEPCGN_25140 0.16 -3.0 3,783,245 + tonB HEPCGN_25140 0.16 +0.3 3,783,338 + tonB HEPCGN_25140 0.29 +0.2 3,783,596 + tonB HEPCGN_25140 0.65 +0.4 3,783,616 + tonB HEPCGN_25140 0.67 -0.2 3,783,854 - +0.5 3,783,890 + -1.0 3,783,890 + +0.8 3,783,902 - +1.4 3,783,902 - -0.7 3,783,938 + yciA HEPCGN_25145 0.12 +2.0 3,783,939 - yciA HEPCGN_25145 0.13 +0.6 3,783,939 - yciA HEPCGN_25145 0.13 +0.8 3,783,942 + yciA HEPCGN_25145 0.13 -1.5 3,783,943 - yciA HEPCGN_25145 0.14 -0.6 3,783,943 - yciA HEPCGN_25145 0.14 +0.1 3,783,943 - yciA HEPCGN_25145 0.14 +0.5 3,783,965 + yciA HEPCGN_25145 0.19 +1.0 3,783,966 - yciA HEPCGN_25145 0.19 -0.3 3,784,014 + yciA HEPCGN_25145 0.31 -0.1 3,784,017 + yciA HEPCGN_25145 0.32 +0.0 3,784,018 - yciA HEPCGN_25145 0.32 -0.7 3,784,094 - yciA HEPCGN_25145 0.51 -0.6 3,784,247 - yciA HEPCGN_25145 0.90 +0.1 3,784,272 + -1.2 3,784,282 - +0.4 3,784,282 - -0.9 3,784,290 + +1.4 3,784,290 + -2.4 3,784,291 - +0.8 3,784,291 - -0.0 3,784,302 - -0.2 3,784,302 - +1.3 3,784,328 - +0.1 3,784,360 - +3.4 3,784,401 - +0.2 3,784,428 - +0.2 3,784,430 - +1.3 3,784,525 + yciB HEPCGN_25150 0.25 -1.5
Or see this region's nucleotide sequence