Strain Fitness in Escherichia coli ECOR38 around HEPCGN_24130

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_24125 and HEPCGN_24130 are separated by 288 nucleotidesHEPCGN_24130 and ssb overlap by 4 nucleotidesssb and HEPCGN_24140 are separated by 9 nucleotidesHEPCGN_24140 and gp36 are separated by 287 nucleotides HEPCGN_24125: HEPCGN_24125 - DNA primase, at 3,610,496 to 3,612,244 _24125 HEPCGN_24130: HEPCGN_24130 - hypothetical protein, at 3,612,533 to 3,612,790 _24130 HEPCGN_24135: ssb - single-stranded DNA-binding protein, at 3,612,787 to 3,613,197 ssb HEPCGN_24140: HEPCGN_24140 - PerC family transcriptional regulator, at 3,613,207 to 3,613,479 _24140 HEPCGN_24145: gp36 - phage major capsid protein, at 3,613,767 to 3,614,924 gp36 Position (kb) 3612 3613Strain fitness (log2 ratio) -1 0 1at 3611.681 kb on - strand, within HEPCGN_24125at 3611.727 kb on + strand, within HEPCGN_24125at 3612.041 kb on + strand, within HEPCGN_24125at 3612.042 kb on - strand, within HEPCGN_24125at 3612.118 kb on - strandat 3612.118 kb on - strandat 3612.171 kb on - strandat 3612.352 kb on - strandat 3612.352 kb on - strandat 3612.402 kb on + strandat 3612.466 kb on - strandat 3612.479 kb on + strandat 3612.750 kb on + strand, within HEPCGN_24130at 3612.837 kb on - strand, within ssbat 3613.019 kb on + strand, within ssbat 3613.212 kb on - strandat 3613.212 kb on - strandat 3613.212 kb on - strandat 3613.212 kb on - strandat 3613.252 kb on + strand, within HEPCGN_24140at 3613.418 kb on - strand, within HEPCGN_24140at 3613.418 kb on - strand, within HEPCGN_24140at 3613.454 kb on + strandat 3613.455 kb on - strandat 3613.465 kb on + strandat 3613.641 kb on + strandat 3613.642 kb on - strandat 3613.642 kb on - strandat 3613.642 kb on - strandat 3613.748 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,611,681 - HEPCGN_24125 0.68 +1.0
3,611,727 + HEPCGN_24125 0.70 +0.4
3,612,041 + HEPCGN_24125 0.88 -1.1
3,612,042 - HEPCGN_24125 0.88 +0.1
3,612,118 - -0.1
3,612,118 - +0.7
3,612,171 - -0.1
3,612,352 - +0.3
3,612,352 - +0.8
3,612,402 + -1.3
3,612,466 - +0.4
3,612,479 + +0.2
3,612,750 + HEPCGN_24130 0.84 -0.7
3,612,837 - ssb HEPCGN_24135 0.12 -0.1
3,613,019 + ssb HEPCGN_24135 0.56 +0.1
3,613,212 - -0.6
3,613,212 - -0.6
3,613,212 - -0.7
3,613,212 - -0.3
3,613,252 + HEPCGN_24140 0.16 +0.5
3,613,418 - HEPCGN_24140 0.77 -0.4
3,613,418 - HEPCGN_24140 0.77 +0.3
3,613,454 + -0.3
3,613,455 - -1.6
3,613,465 + -1.1
3,613,641 + -0.2
3,613,642 - -0.1
3,613,642 - +0.4
3,613,642 - +0.5
3,613,748 + -1.2

Or see this region's nucleotide sequence