Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_24125 and HEPCGN_24130 are separated by 288 nucleotides HEPCGN_24130 and ssb overlap by 4 nucleotides ssb and HEPCGN_24140 are separated by 9 nucleotides HEPCGN_24140 and gp36 are separated by 287 nucleotides
HEPCGN_24125: HEPCGN_24125 - DNA primase, at 3,610,496 to 3,612,244
_24125
HEPCGN_24130: HEPCGN_24130 - hypothetical protein, at 3,612,533 to 3,612,790
_24130
HEPCGN_24135: ssb - single-stranded DNA-binding protein, at 3,612,787 to 3,613,197
ssb
HEPCGN_24140: HEPCGN_24140 - PerC family transcriptional regulator, at 3,613,207 to 3,613,479
_24140
HEPCGN_24145: gp36 - phage major capsid protein, at 3,613,767 to 3,614,924
gp36
Position (kb)
3612
3613 Strain fitness (log2 ratio)
-1
0
1 at 3611.681 kb on - strand, within HEPCGN_24125 at 3611.727 kb on + strand, within HEPCGN_24125 at 3612.041 kb on + strand, within HEPCGN_24125 at 3612.042 kb on - strand, within HEPCGN_24125 at 3612.118 kb on - strand at 3612.118 kb on - strand at 3612.171 kb on - strand at 3612.352 kb on - strand at 3612.352 kb on - strand at 3612.402 kb on + strand at 3612.466 kb on - strand at 3612.479 kb on + strand at 3612.750 kb on + strand, within HEPCGN_24130 at 3612.837 kb on - strand, within ssb at 3613.019 kb on + strand, within ssb at 3613.212 kb on - strand at 3613.212 kb on - strand at 3613.212 kb on - strand at 3613.212 kb on - strand at 3613.252 kb on + strand, within HEPCGN_24140 at 3613.418 kb on - strand, within HEPCGN_24140 at 3613.418 kb on - strand, within HEPCGN_24140 at 3613.454 kb on + strand at 3613.455 kb on - strand at 3613.465 kb on + strand at 3613.641 kb on + strand at 3613.642 kb on - strand at 3613.642 kb on - strand at 3613.642 kb on - strand at 3613.748 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 3,611,681 - HEPCGN_24125 0.68 +1.0 3,611,727 + HEPCGN_24125 0.70 +0.4 3,612,041 + HEPCGN_24125 0.88 -1.1 3,612,042 - HEPCGN_24125 0.88 +0.1 3,612,118 - -0.1 3,612,118 - +0.7 3,612,171 - -0.1 3,612,352 - +0.3 3,612,352 - +0.8 3,612,402 + -1.3 3,612,466 - +0.4 3,612,479 + +0.2 3,612,750 + HEPCGN_24130 0.84 -0.7 3,612,837 - ssb HEPCGN_24135 0.12 -0.1 3,613,019 + ssb HEPCGN_24135 0.56 +0.1 3,613,212 - -0.6 3,613,212 - -0.6 3,613,212 - -0.7 3,613,212 - -0.3 3,613,252 + HEPCGN_24140 0.16 +0.5 3,613,418 - HEPCGN_24140 0.77 -0.4 3,613,418 - HEPCGN_24140 0.77 +0.3 3,613,454 + -0.3 3,613,455 - -1.6 3,613,465 + -1.1 3,613,641 + -0.2 3,613,642 - -0.1 3,613,642 - +0.4 3,613,642 - +0.5 3,613,748 + -1.2
Or see this region's nucleotide sequence