Strain Fitness in Escherichia coli ECOR38 around HEPCGN_24040

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolE and nagK are separated by 28 nucleotidesnagK and cobB are separated by 15 nucleotidescobB and HEPCGN_24050 are separated by 137 nucleotides HEPCGN_24035: lolE - lipoprotein-releasing ABC transporter permease subunit LolE, at 3,595,355 to 3,596,593 lolE HEPCGN_24040: nagK - N-acetylglucosamine kinase, at 3,596,622 to 3,597,533 nagK HEPCGN_24045: cobB - NAD-dependent protein deacylase, at 3,597,549 to 3,598,370 cobB HEPCGN_24050: HEPCGN_24050 - Inner membrane protein YcfZ, at 3,598,508 to 3,599,296 _24050 Position (kb) 3596 3597 3598Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3596.592 kb on - strandat 3596.605 kb on + strandat 3596.606 kb on - strandat 3596.606 kb on - strandat 3596.625 kb on + strandat 3596.625 kb on + strandat 3596.626 kb on - strandat 3596.727 kb on + strand, within nagKat 3596.727 kb on + strand, within nagKat 3596.728 kb on - strand, within nagKat 3596.762 kb on - strand, within nagKat 3596.841 kb on - strand, within nagKat 3596.848 kb on - strand, within nagKat 3596.877 kb on - strand, within nagKat 3596.921 kb on - strand, within nagKat 3597.069 kb on + strand, within nagKat 3597.069 kb on + strand, within nagKat 3597.071 kb on + strand, within nagKat 3597.072 kb on - strand, within nagKat 3597.072 kb on - strand, within nagKat 3597.072 kb on - strand, within nagKat 3597.075 kb on - strand, within nagKat 3597.183 kb on + strand, within nagKat 3597.214 kb on - strand, within nagKat 3597.223 kb on - strand, within nagKat 3597.225 kb on + strand, within nagKat 3597.226 kb on - strand, within nagKat 3597.339 kb on - strand, within nagKat 3597.442 kb on + strandat 3597.443 kb on - strandat 3597.517 kb on + strandat 3597.517 kb on + strandat 3597.518 kb on - strandat 3597.518 kb on - strandat 3597.523 kb on + strandat 3597.524 kb on - strandat 3597.548 kb on + strandat 3597.548 kb on + strandat 3597.641 kb on + strand, within cobBat 3597.717 kb on - strand, within cobBat 3597.822 kb on + strand, within cobBat 3597.977 kb on + strand, within cobBat 3597.984 kb on - strand, within cobBat 3597.984 kb on - strand, within cobBat 3597.984 kb on - strand, within cobBat 3598.109 kb on - strand, within cobBat 3598.109 kb on - strand, within cobBat 3598.149 kb on + strand, within cobBat 3598.150 kb on - strand, within cobBat 3598.191 kb on - strand, within cobBat 3598.206 kb on + strand, within cobBat 3598.368 kb on + strandat 3598.484 kb on + strandat 3598.526 kb on + strandat 3598.526 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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3,596,592 - -0.2
3,596,605 + -1.3
3,596,606 - +0.6
3,596,606 - -0.7
3,596,625 + -0.8
3,596,625 + +0.2
3,596,626 - +0.3
3,596,727 + nagK HEPCGN_24040 0.12 +1.0
3,596,727 + nagK HEPCGN_24040 0.12 -0.1
3,596,728 - nagK HEPCGN_24040 0.12 +1.0
3,596,762 - nagK HEPCGN_24040 0.15 -1.0
3,596,841 - nagK HEPCGN_24040 0.24 -1.2
3,596,848 - nagK HEPCGN_24040 0.25 +0.8
3,596,877 - nagK HEPCGN_24040 0.28 +0.3
3,596,921 - nagK HEPCGN_24040 0.33 +0.6
3,597,069 + nagK HEPCGN_24040 0.49 -0.2
3,597,069 + nagK HEPCGN_24040 0.49 -0.2
3,597,071 + nagK HEPCGN_24040 0.49 +0.6
3,597,072 - nagK HEPCGN_24040 0.49 -0.9
3,597,072 - nagK HEPCGN_24040 0.49 +0.0
3,597,072 - nagK HEPCGN_24040 0.49 +0.5
3,597,075 - nagK HEPCGN_24040 0.50 +1.0
3,597,183 + nagK HEPCGN_24040 0.62 +0.4
3,597,214 - nagK HEPCGN_24040 0.65 +2.5
3,597,223 - nagK HEPCGN_24040 0.66 +0.0
3,597,225 + nagK HEPCGN_24040 0.66 +0.4
3,597,226 - nagK HEPCGN_24040 0.66 -0.4
3,597,339 - nagK HEPCGN_24040 0.79 +0.4
3,597,442 + +1.8
3,597,443 - -0.1
3,597,517 + -0.5
3,597,517 + +0.4
3,597,518 - -0.4
3,597,518 - +0.9
3,597,523 + +0.2
3,597,524 - +0.1
3,597,548 + +0.1
3,597,548 + -0.1
3,597,641 + cobB HEPCGN_24045 0.11 -0.8
3,597,717 - cobB HEPCGN_24045 0.20 -1.5
3,597,822 + cobB HEPCGN_24045 0.33 -0.5
3,597,977 + cobB HEPCGN_24045 0.52 -0.7
3,597,984 - cobB HEPCGN_24045 0.53 +0.5
3,597,984 - cobB HEPCGN_24045 0.53 +0.3
3,597,984 - cobB HEPCGN_24045 0.53 -0.3
3,598,109 - cobB HEPCGN_24045 0.68 +0.1
3,598,109 - cobB HEPCGN_24045 0.68 +0.8
3,598,149 + cobB HEPCGN_24045 0.73 -3.0
3,598,150 - cobB HEPCGN_24045 0.73 +0.6
3,598,191 - cobB HEPCGN_24045 0.78 -0.5
3,598,206 + cobB HEPCGN_24045 0.80 +0.3
3,598,368 + -0.3
3,598,484 + -0.7
3,598,526 + -0.0
3,598,526 + +0.4

Or see this region's nucleotide sequence