Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspG and gspH are separated by 3 nucleotides gspH and gspI overlap by 4 nucleotides gspI and gspJ overlap by 4 nucleotides gspJ and gspK overlap by 4 nucleotides gspK and gspL overlap by 4 nucleotides
HEPCGN_18065: gspG - general secretion pathway protein G, at 2,363,713 to 2,364,168
gspG
HEPCGN_18070: gspH - general secretion pathway protein H, at 2,364,172 to 2,364,735
gspH
HEPCGN_18075: gspI - general secretion pathway protein I, at 2,364,732 to 2,365,103
gspI
HEPCGN_18080: gspJ - general secretion pathway protein J, at 2,365,100 to 2,365,705
gspJ
HEPCGN_18085: gspK - general secretion pathway protein K, at 2,365,702 to 2,366,679
gspK
HEPCGN_18090: gspL - general secretion pathway protein L, at 2,366,676 to 2,367,854
gspL
Position (kb)
2365
2366 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2364.141 kb on + strand at 2364.382 kb on + strand, within gspH at 2364.451 kb on - strand, within gspH at 2364.480 kb on - strand, within gspH at 2364.496 kb on - strand, within gspH at 2364.511 kb on + strand, within gspH at 2364.512 kb on - strand, within gspH at 2364.634 kb on - strand, within gspH at 2364.647 kb on - strand, within gspH at 2364.701 kb on + strand at 2364.746 kb on + strand at 2364.747 kb on - strand at 2364.805 kb on + strand, within gspI at 2364.805 kb on + strand, within gspI at 2364.806 kb on - strand, within gspI at 2364.831 kb on - strand, within gspI at 2364.898 kb on - strand, within gspI at 2365.341 kb on + strand, within gspJ at 2365.572 kb on - strand, within gspJ at 2365.630 kb on - strand, within gspJ at 2366.016 kb on + strand, within gspK at 2366.225 kb on - strand, within gspK at 2366.355 kb on + strand, within gspK at 2366.356 kb on - strand, within gspK at 2366.356 kb on - strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.360 kb on + strand, within gspK at 2366.361 kb on - strand, within gspK at 2366.361 kb on - strand, within gspK at 2366.436 kb on - strand, within gspK at 2366.529 kb on - strand, within gspK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 2,364,141 + -1.9 2,364,382 + gspH HEPCGN_18070 0.37 +0.2 2,364,451 - gspH HEPCGN_18070 0.49 -0.1 2,364,480 - gspH HEPCGN_18070 0.55 -0.0 2,364,496 - gspH HEPCGN_18070 0.57 +0.9 2,364,511 + gspH HEPCGN_18070 0.60 +0.2 2,364,512 - gspH HEPCGN_18070 0.60 +0.4 2,364,634 - gspH HEPCGN_18070 0.82 -0.2 2,364,647 - gspH HEPCGN_18070 0.84 +0.3 2,364,701 + +1.2 2,364,746 + +0.7 2,364,747 - -0.5 2,364,805 + gspI HEPCGN_18075 0.20 -0.3 2,364,805 + gspI HEPCGN_18075 0.20 +0.1 2,364,806 - gspI HEPCGN_18075 0.20 -0.5 2,364,831 - gspI HEPCGN_18075 0.27 +0.1 2,364,898 - gspI HEPCGN_18075 0.45 +0.7 2,365,341 + gspJ HEPCGN_18080 0.40 -0.3 2,365,572 - gspJ HEPCGN_18080 0.78 -2.0 2,365,630 - gspJ HEPCGN_18080 0.87 -0.6 2,366,016 + gspK HEPCGN_18085 0.32 -0.1 2,366,225 - gspK HEPCGN_18085 0.53 +2.2 2,366,355 + gspK HEPCGN_18085 0.67 -0.2 2,366,356 - gspK HEPCGN_18085 0.67 -0.0 2,366,356 - gspK HEPCGN_18085 0.67 -0.4 2,366,360 + gspK HEPCGN_18085 0.67 +0.6 2,366,360 + gspK HEPCGN_18085 0.67 +1.7 2,366,360 + gspK HEPCGN_18085 0.67 +0.0 2,366,360 + gspK HEPCGN_18085 0.67 +2.6 2,366,360 + gspK HEPCGN_18085 0.67 +0.3 2,366,360 + gspK HEPCGN_18085 0.67 -1.2 2,366,361 - gspK HEPCGN_18085 0.67 -0.2 2,366,361 - gspK HEPCGN_18085 0.67 -0.2 2,366,436 - gspK HEPCGN_18085 0.75 -2.4 2,366,529 - gspK HEPCGN_18085 0.85 -1.0
Or see this region's nucleotide sequence