Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HEPCGN_07700 and ykgR are separated by 124 nucleotides ykgR and rpmE2-1 are separated by 361 nucleotides rpmE2-1 and HEPCGN_07715 overlap by 1 nucleotides
HEPCGN_07700: HEPCGN_07700 - hypothetical protein, at 285,861 to 285,980
_07700
HEPCGN_07705: ykgR - Uncharacterized membrane protein YkgR, at 286,105 to 286,206
ykgR
HEPCGN_07710: rpmE2-1 - type B 50S ribosomal protein L31, at 286,568 to 286,834
rpmE2-1
HEPCGN_07715: HEPCGN_07715 - 50S ribosomal protein L36 2, at 286,834 to 286,974
_07715
Position (kb) 286
287 Strain fitness (log2 ratio) -1
0
1 at 285.835 kb on + strand at 285.836 kb on - strand at 285.861 kb on - strand at 285.957 kb on - strand, within HEPCGN_07700 at 285.957 kb on - strand, within HEPCGN_07700 at 286.050 kb on + strand at 286.741 kb on + strand, within rpmE2-1 at 286.941 kb on + strand, within HEPCGN_07715 at 286.941 kb on + strand, within HEPCGN_07715 at 286.959 kb on + strand, within HEPCGN_07715 at 287.121 kb on - strand at 287.131 kb on - strand at 287.165 kb on - strand at 287.187 kb on - strand at 287.236 kb on + strand at 287.258 kb on + strand at 287.287 kb on + strand at 287.446 kb on + strand at 287.446 kb on + strand at 287.447 kb on - strand at 287.447 kb on - strand at 287.488 kb on + strand at 287.614 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 285,835 + -0.7 285,836 - +0.0 285,861 - -0.2 285,957 - HEPCGN_07700 0.80 -0.3 285,957 - HEPCGN_07700 0.80 -0.1 286,050 + +1.2 286,741 + rpmE2-1 HEPCGN_07710 0.65 -0.8 286,941 + HEPCGN_07715 0.76 -1.4 286,941 + HEPCGN_07715 0.76 +1.2 286,959 + HEPCGN_07715 0.89 -1.6 287,121 - +0.0 287,131 - +0.6 287,165 - -1.4 287,187 - +1.1 287,236 + -0.8 287,258 + -0.9 287,287 + +0.2 287,446 + +0.0 287,446 + -1.4 287,447 - +0.7 287,447 - -0.7 287,488 + +0.8 287,614 - -0.2
Or see this region's nucleotide sequence