Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gdhA and ynjH are separated by 235 nucleotides ynjH and nudG overlap by 35 nucleotides nudG and ynjF are separated by 86 nucleotides ynjF and ynjE are separated by 8 nucleotides
HEPCGN_05205: gdhA - NADP-specific glutamate dehydrogenase, at 918,097 to 919,440
gdhA
HEPCGN_05210: ynjH - Uncharacterized protein YnjH, at 919,676 to 919,948
ynjH
HEPCGN_05215: nudG - CTP pyrophosphohydrolase, at 919,914 to 920,321
nudG
HEPCGN_05220: ynjF - Inner membrane protein YnjF, at 920,408 to 921,028
ynjF
HEPCGN_05225: ynjE - thiosulfate sulfurtransferase YnjE, at 921,037 to 922,344
ynjE
Position (kb) 919
920
921 Strain fitness (log2 ratio) -2
-1
0
1
2 at 919.081 kb on - strand, within gdhA at 919.219 kb on + strand, within gdhA at 919.219 kb on + strand, within gdhA at 919.349 kb on - strand at 919.441 kb on - strand at 919.444 kb on + strand at 919.457 kb on + strand at 919.458 kb on - strand at 919.459 kb on + strand at 919.486 kb on + strand at 919.516 kb on + strand at 919.569 kb on + strand at 919.581 kb on + strand at 919.590 kb on - strand at 919.646 kb on - strand at 919.704 kb on + strand, within ynjH at 919.705 kb on - strand, within ynjH at 919.739 kb on + strand, within ynjH at 919.827 kb on - strand, within ynjH at 919.834 kb on + strand, within ynjH at 919.862 kb on - strand, within ynjH at 919.960 kb on - strand, within nudG at 920.065 kb on - strand, within nudG at 920.125 kb on + strand, within nudG at 920.125 kb on + strand, within nudG at 920.126 kb on - strand, within nudG at 920.172 kb on + strand, within nudG at 920.173 kb on - strand, within nudG at 920.326 kb on + strand at 920.360 kb on - strand at 920.390 kb on + strand at 920.391 kb on - strand at 920.391 kb on - strand at 920.393 kb on - strand at 920.393 kb on - strand at 920.413 kb on - strand at 920.506 kb on - strand, within ynjF at 920.558 kb on + strand, within ynjF at 920.842 kb on + strand, within ynjF at 920.855 kb on + strand, within ynjF at 920.947 kb on - strand, within ynjF at 921.039 kb on - strand at 921.104 kb on + strand at 921.155 kb on + strand at 921.156 kb on - strand at 921.156 kb on - strand at 921.159 kb on + strand at 921.159 kb on + strand at 921.194 kb on + strand, within ynjE at 921.240 kb on + strand, within ynjE at 921.246 kb on + strand, within ynjE at 921.297 kb on + strand, within ynjE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 919,081 - gdhA HEPCGN_05205 0.73 +0.1 919,219 + gdhA HEPCGN_05205 0.83 -1.2 919,219 + gdhA HEPCGN_05205 0.83 -0.4 919,349 - -2.5 919,441 - +0.8 919,444 + +1.3 919,457 + -0.1 919,458 - -2.6 919,459 + -1.0 919,486 + -0.5 919,516 + -1.0 919,569 + -1.0 919,581 + -0.2 919,590 - +1.1 919,646 - +0.5 919,704 + ynjH HEPCGN_05210 0.10 -1.2 919,705 - ynjH HEPCGN_05210 0.11 +0.1 919,739 + ynjH HEPCGN_05210 0.23 -1.0 919,827 - ynjH HEPCGN_05210 0.55 -1.1 919,834 + ynjH HEPCGN_05210 0.58 +0.4 919,862 - ynjH HEPCGN_05210 0.68 -0.4 919,960 - nudG HEPCGN_05215 0.11 -0.9 920,065 - nudG HEPCGN_05215 0.37 -0.3 920,125 + nudG HEPCGN_05215 0.52 -0.8 920,125 + nudG HEPCGN_05215 0.52 -0.9 920,126 - nudG HEPCGN_05215 0.52 +0.2 920,172 + nudG HEPCGN_05215 0.63 -2.0 920,173 - nudG HEPCGN_05215 0.63 -0.8 920,326 + -0.1 920,360 - +0.5 920,390 + -0.1 920,391 - -0.4 920,391 - -0.7 920,393 - -0.5 920,393 - +0.6 920,413 - +0.6 920,506 - ynjF HEPCGN_05220 0.16 +0.1 920,558 + ynjF HEPCGN_05220 0.24 +0.1 920,842 + ynjF HEPCGN_05220 0.70 +0.6 920,855 + ynjF HEPCGN_05220 0.72 +0.0 920,947 - ynjF HEPCGN_05220 0.87 +0.7 921,039 - +0.6 921,104 + +0.2 921,155 + +0.8 921,156 - -0.5 921,156 - -1.7 921,159 + -1.8 921,159 + -0.3 921,194 + ynjE HEPCGN_05225 0.12 +0.1 921,240 + ynjE HEPCGN_05225 0.16 -1.1 921,246 + ynjE HEPCGN_05225 0.16 +2.5 921,297 + ynjE HEPCGN_05225 0.20 -1.2
Or see this region's nucleotide sequence