Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and nudB are separated by 1 nucleotides nudB and HEPCGN_04665 are separated by 28 nucleotides HEPCGN_04665 and HEPCGN_04670 overlap by 1 nucleotides HEPCGN_04670 and ruvC overlap by 1 nucleotides
HEPCGN_04655: aspS - aspartate--tRNA ligase, at 812,411 to 814,183
aspS
HEPCGN_04660: nudB - dihydroneopterin triphosphate diphosphatase, at 814,185 to 814,628
nudB
HEPCGN_04665: HEPCGN_04665 - putative transcriptional regulatory protein YebC, at 814,657 to 815,397
_04665
HEPCGN_04670: HEPCGN_04670 - Crossover junction endodeoxyribonuclease RuvC, at 815,397 to 815,432
_04670
HEPCGN_04675: ruvC - crossover junction endodeoxyribonuclease RuvC, at 815,432 to 815,953
ruvC
Position (kb) 814
815 Strain fitness (log2 ratio) -1
0
1
2 at 814.192 kb on - strand at 814.192 kb on - strand at 814.346 kb on - strand, within nudB at 814.346 kb on - strand, within nudB at 814.346 kb on - strand, within nudB at 814.435 kb on + strand, within nudB at 814.536 kb on + strand, within nudB at 814.612 kb on + strand at 814.616 kb on + strand at 814.656 kb on + strand at 814.669 kb on - strand at 814.818 kb on + strand, within HEPCGN_04665 at 814.916 kb on - strand, within HEPCGN_04665 at 815.050 kb on + strand, within HEPCGN_04665 at 815.277 kb on + strand, within HEPCGN_04665 at 815.358 kb on + strand at 815.440 kb on + strand at 815.577 kb on - strand, within ruvC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 814,192 - +0.3 814,192 - +2.7 814,346 - nudB HEPCGN_04660 0.36 -0.8 814,346 - nudB HEPCGN_04660 0.36 -0.2 814,346 - nudB HEPCGN_04660 0.36 +0.0 814,435 + nudB HEPCGN_04660 0.56 -1.4 814,536 + nudB HEPCGN_04660 0.79 -0.9 814,612 + -0.5 814,616 + -0.6 814,656 + -0.4 814,669 - -0.1 814,818 + HEPCGN_04665 0.22 +1.1 814,916 - HEPCGN_04665 0.35 -0.6 815,050 + HEPCGN_04665 0.53 +1.1 815,277 + HEPCGN_04665 0.84 +0.1 815,358 + +0.7 815,440 + +0.2 815,577 - ruvC HEPCGN_04675 0.28 +0.7
Or see this region's nucleotide sequence