Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03055

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntHEPCGN_03045 and fcl are separated by 2 nucleotidesfcl and gmm overlap by 1 nucleotidesgmm and wcaI overlap by 4 nucleotides HEPCGN_03045: HEPCGN_03045 - GDP-mannose 4,6-dehydratase, at 517,523 to 518,644 _03045 HEPCGN_03050: fcl - GDP-L-fucose synthase, at 518,647 to 519,612 fcl HEPCGN_03055: gmm - GDP-mannose mannosyl hydrolase, at 519,612 to 520,094 gmm HEPCGN_03060: wcaI - colanic acid biosynthesis fucosyltransferase WcaI, at 520,091 to 521,314 wcaI Position (kb) 519 520 521Strain fitness (log2 ratio) -2 -1 0 1at 518.652 kb on + strandat 518.667 kb on + strandat 518.741 kb on + strandat 518.741 kb on + strandat 518.741 kb on + strandat 518.745 kb on - strand, within fclat 518.745 kb on - strand, within fclat 518.745 kb on - strand, within fclat 518.812 kb on - strand, within fclat 519.174 kb on + strand, within fclat 519.483 kb on + strand, within fclat 519.571 kb on + strandat 519.610 kb on + strandat 519.647 kb on - strandat 519.847 kb on + strand, within gmmat 519.848 kb on - strand, within gmmat 519.848 kb on - strand, within gmmat 519.887 kb on + strand, within gmmat 519.888 kb on - strand, within gmmat 519.919 kb on - strand, within gmmat 519.919 kb on - strand, within gmmat 520.098 kb on + strandat 520.148 kb on + strandat 520.148 kb on + strandat 520.149 kb on - strandat 520.201 kb on + strandat 520.247 kb on + strand, within wcaIat 520.594 kb on + strand, within wcaIat 520.648 kb on + strand, within wcaIat 520.649 kb on - strand, within wcaIat 520.797 kb on - strand, within wcaIat 520.807 kb on + strand, within wcaIat 520.837 kb on + strand, within wcaIat 521.050 kb on - strand, within wcaI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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518,652 + -1.0
518,667 + -1.7
518,741 + +1.1
518,741 + -2.2
518,741 + +0.4
518,745 - fcl HEPCGN_03050 0.10 +0.4
518,745 - fcl HEPCGN_03050 0.10 -0.1
518,745 - fcl HEPCGN_03050 0.10 +1.2
518,812 - fcl HEPCGN_03050 0.17 +0.6
519,174 + fcl HEPCGN_03050 0.55 +0.1
519,483 + fcl HEPCGN_03050 0.87 +0.3
519,571 + -0.3
519,610 + -0.9
519,647 - +0.6
519,847 + gmm HEPCGN_03055 0.49 -1.3
519,848 - gmm HEPCGN_03055 0.49 +0.4
519,848 - gmm HEPCGN_03055 0.49 -0.1
519,887 + gmm HEPCGN_03055 0.57 -1.7
519,888 - gmm HEPCGN_03055 0.57 -2.1
519,919 - gmm HEPCGN_03055 0.64 -1.3
519,919 - gmm HEPCGN_03055 0.64 -0.9
520,098 + -0.9
520,148 + -0.1
520,148 + -0.4
520,149 - +1.6
520,201 + +0.9
520,247 + wcaI HEPCGN_03060 0.13 -0.6
520,594 + wcaI HEPCGN_03060 0.41 +1.0
520,648 + wcaI HEPCGN_03060 0.46 +1.2
520,649 - wcaI HEPCGN_03060 0.46 -0.0
520,797 - wcaI HEPCGN_03060 0.58 +0.4
520,807 + wcaI HEPCGN_03060 0.58 +0.2
520,837 + wcaI HEPCGN_03060 0.61 -2.0
521,050 - wcaI HEPCGN_03060 0.78 +0.5

Or see this region's nucleotide sequence