Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rcnA and rcnR are separated by 120 nucleotides rcnR and thiM are separated by 222 nucleotides thiM and HEPCGN_02760 overlap by 4 nucleotides HEPCGN_02760 and yegX are separated by 64 nucleotides
HEPCGN_02745: rcnA - Nickel/cobalt efflux system RcnA, at 459,996 to 460,793
rcnA
HEPCGN_02750: rcnR - Ni(II)/Co(II)-binding transcriptional repressor RcnR, at 460,914 to 461,186
rcnR
HEPCGN_02755: thiM - hydroxyethylthiazole kinase, at 461,409 to 462,197
thiM
HEPCGN_02760: HEPCGN_02760 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 462,194 to 462,994
_02760
HEPCGN_02765: yegX - Uncharacterized protein YegX, at 463,059 to 463,877
yegX
Position (kb) 461
462
463 Strain fitness (log2 ratio) -2
-1
0
1
2 at 460.499 kb on + strand, within rcnA at 460.604 kb on + strand, within rcnA at 460.729 kb on - strand at 460.873 kb on - strand at 461.029 kb on + strand, within rcnR at 461.184 kb on + strand at 461.227 kb on - strand at 461.247 kb on + strand at 461.248 kb on - strand at 461.326 kb on - strand at 461.408 kb on + strand at 461.408 kb on + strand at 461.489 kb on + strand, within thiM at 461.525 kb on + strand, within thiM at 461.935 kb on - strand, within thiM at 462.206 kb on - strand at 462.266 kb on - strand at 462.304 kb on + strand, within HEPCGN_02760 at 462.431 kb on - strand, within HEPCGN_02760 at 462.494 kb on + strand, within HEPCGN_02760 at 462.514 kb on + strand, within HEPCGN_02760 at 462.715 kb on - strand, within HEPCGN_02760 at 462.764 kb on - strand, within HEPCGN_02760 at 462.803 kb on - strand, within HEPCGN_02760 at 462.832 kb on + strand, within HEPCGN_02760 at 462.833 kb on - strand, within HEPCGN_02760 at 462.833 kb on - strand, within HEPCGN_02760 at 463.001 kb on + strand at 463.004 kb on + strand at 463.005 kb on - strand at 463.005 kb on - strand at 463.005 kb on - strand at 463.005 kb on - strand at 463.007 kb on - strand at 463.117 kb on + strand at 463.165 kb on - strand, within yegX
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 460,499 + rcnA HEPCGN_02745 0.63 -0.6 460,604 + rcnA HEPCGN_02745 0.76 -0.6 460,729 - +0.0 460,873 - -0.1 461,029 + rcnR HEPCGN_02750 0.42 +0.1 461,184 + +0.2 461,227 - +0.9 461,247 + -0.2 461,248 - +0.1 461,326 - +0.0 461,408 + -0.1 461,408 + -0.7 461,489 + thiM HEPCGN_02755 0.10 -0.1 461,525 + thiM HEPCGN_02755 0.15 -1.0 461,935 - thiM HEPCGN_02755 0.67 -2.1 462,206 - +0.9 462,266 - +1.9 462,304 + HEPCGN_02760 0.14 -1.1 462,431 - HEPCGN_02760 0.30 -0.1 462,494 + HEPCGN_02760 0.37 -0.8 462,514 + HEPCGN_02760 0.40 +0.7 462,715 - HEPCGN_02760 0.65 +0.2 462,764 - HEPCGN_02760 0.71 -0.6 462,803 - HEPCGN_02760 0.76 -0.2 462,832 + HEPCGN_02760 0.80 +0.2 462,833 - HEPCGN_02760 0.80 -1.1 462,833 - HEPCGN_02760 0.80 +0.1 463,001 + +0.6 463,004 + -0.3 463,005 - -0.2 463,005 - +1.2 463,005 - +2.5 463,005 - +0.2 463,007 - -0.3 463,117 + -0.3 463,165 - yegX HEPCGN_02765 0.13 +0.4
Or see this region's nucleotide sequence