Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeI and dgcI are separated by 7 nucleotides dgcI and yiaG are separated by 69 nucleotides yiaG and HEPCGN_01965 overlap by 11 nucleotides
HEPCGN_01950: pdeI - putative cyclic di-GMP phosphodiesterase PdeI, at 316,613 to 318,961
pdeI
HEPCGN_01955: dgcI - putative diguanylate cyclase DgcI, at 318,969 to 320,297
dgcI
HEPCGN_01960: yiaG - transcriptional regulator, at 320,367 to 320,696
yiaG
HEPCGN_01965: HEPCGN_01965 - addiction module toxin RelE, at 320,686 to 321,072
_01965
Position (kb) 318
319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 318.136 kb on + strand, within pdeI at 318.184 kb on - strand, within pdeI at 318.251 kb on + strand, within pdeI at 318.252 kb on - strand, within pdeI at 318.271 kb on + strand, within pdeI at 318.437 kb on - strand, within pdeI at 318.527 kb on - strand, within pdeI at 318.640 kb on + strand, within pdeI at 318.721 kb on + strand, within pdeI at 318.806 kb on + strand at 318.896 kb on + strand at 318.897 kb on - strand at 318.968 kb on + strand at 318.996 kb on - strand at 319.140 kb on - strand, within dgcI at 319.236 kb on + strand, within dgcI at 319.236 kb on + strand, within dgcI at 319.236 kb on + strand, within dgcI at 319.237 kb on - strand, within dgcI at 319.393 kb on - strand, within dgcI at 319.395 kb on + strand, within dgcI at 319.460 kb on - strand, within dgcI at 319.520 kb on + strand, within dgcI at 319.542 kb on + strand, within dgcI at 319.664 kb on + strand, within dgcI at 319.743 kb on - strand, within dgcI at 319.767 kb on - strand, within dgcI at 319.833 kb on + strand, within dgcI at 319.838 kb on + strand, within dgcI at 319.842 kb on + strand, within dgcI at 319.842 kb on + strand, within dgcI at 319.956 kb on - strand, within dgcI at 320.052 kb on - strand, within dgcI at 320.052 kb on - strand, within dgcI at 320.097 kb on + strand, within dgcI at 320.100 kb on - strand, within dgcI at 320.255 kb on + strand at 320.406 kb on - strand, within yiaG at 320.414 kb on + strand, within yiaG at 320.448 kb on - strand, within yiaG at 320.489 kb on + strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.490 kb on - strand, within yiaG at 320.746 kb on - strand, within HEPCGN_01965 at 321.022 kb on - strand, within HEPCGN_01965 at 321.062 kb on + strand at 321.062 kb on + strand at 321.063 kb on - strand at 321.072 kb on + strand at 321.073 kb on - strand at 321.073 kb on - strand at 321.229 kb on - strand at 321.269 kb on + strand at 321.269 kb on + strand at 321.278 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 318,136 + pdeI HEPCGN_01950 0.65 -0.7 318,184 - pdeI HEPCGN_01950 0.67 +0.1 318,251 + pdeI HEPCGN_01950 0.70 -1.2 318,252 - pdeI HEPCGN_01950 0.70 +1.0 318,271 + pdeI HEPCGN_01950 0.71 -0.8 318,437 - pdeI HEPCGN_01950 0.78 -0.1 318,527 - pdeI HEPCGN_01950 0.81 -3.1 318,640 + pdeI HEPCGN_01950 0.86 +0.0 318,721 + pdeI HEPCGN_01950 0.90 +4.1 318,806 + +1.1 318,896 + +0.3 318,897 - +1.1 318,968 + +0.3 318,996 - -0.5 319,140 - dgcI HEPCGN_01955 0.13 -0.6 319,236 + dgcI HEPCGN_01955 0.20 -0.1 319,236 + dgcI HEPCGN_01955 0.20 -0.2 319,236 + dgcI HEPCGN_01955 0.20 -0.3 319,237 - dgcI HEPCGN_01955 0.20 -0.7 319,393 - dgcI HEPCGN_01955 0.32 -0.1 319,395 + dgcI HEPCGN_01955 0.32 -0.1 319,460 - dgcI HEPCGN_01955 0.37 -1.3 319,520 + dgcI HEPCGN_01955 0.41 -0.9 319,542 + dgcI HEPCGN_01955 0.43 +0.5 319,664 + dgcI HEPCGN_01955 0.52 -0.6 319,743 - dgcI HEPCGN_01955 0.58 -0.4 319,767 - dgcI HEPCGN_01955 0.60 +0.6 319,833 + dgcI HEPCGN_01955 0.65 -0.4 319,838 + dgcI HEPCGN_01955 0.65 +0.2 319,842 + dgcI HEPCGN_01955 0.66 +1.2 319,842 + dgcI HEPCGN_01955 0.66 -0.4 319,956 - dgcI HEPCGN_01955 0.74 -0.3 320,052 - dgcI HEPCGN_01955 0.81 -0.7 320,052 - dgcI HEPCGN_01955 0.81 -1.9 320,097 + dgcI HEPCGN_01955 0.85 -2.0 320,100 - dgcI HEPCGN_01955 0.85 -1.4 320,255 + +2.1 320,406 - yiaG HEPCGN_01960 0.12 +1.0 320,414 + yiaG HEPCGN_01960 0.14 -0.2 320,448 - yiaG HEPCGN_01960 0.25 +0.6 320,489 + yiaG HEPCGN_01960 0.37 -1.8 320,490 - yiaG HEPCGN_01960 0.37 +0.2 320,490 - yiaG HEPCGN_01960 0.37 -1.5 320,490 - yiaG HEPCGN_01960 0.37 +1.2 320,490 - yiaG HEPCGN_01960 0.37 +0.9 320,746 - HEPCGN_01965 0.16 -0.1 321,022 - HEPCGN_01965 0.87 -0.6 321,062 + +0.4 321,062 + +0.6 321,063 - +0.4 321,072 + +0.2 321,073 - -0.1 321,073 - +0.3 321,229 - +1.9 321,269 + +0.9 321,269 + +1.6 321,278 + -0.2
Or see this region's nucleotide sequence