Strain Fitness in Escherichia coli ECOR38 around HEPCGN_01455

Experiment: Bas67

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybgC and tolQ overlap by 4 nucleotidestolQ and tolR are separated by 3 nucleotidestolR and tolA are separated by 64 nucleotides HEPCGN_01445: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 219,628 to 220,032 ybgC HEPCGN_01450: tolQ - Tol-Pal system protein TolQ, at 220,029 to 220,721 tolQ HEPCGN_01455: tolR - colicin uptake protein TolR, at 220,725 to 221,153 tolR HEPCGN_01460: tolA - cell envelope integrity protein TolA, at 221,218 to 222,405 tolA Position (kb) 220 221 222Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 219.804 kb on - strand, within ybgCat 219.808 kb on - strand, within ybgCat 219.827 kb on - strand, within ybgCat 219.872 kb on + strand, within ybgCat 219.912 kb on + strand, within ybgCat 219.913 kb on - strand, within ybgCat 219.930 kb on + strand, within ybgCat 220.009 kb on - strandat 220.043 kb on + strandat 220.044 kb on - strandat 220.224 kb on + strand, within tolQat 220.367 kb on + strand, within tolQat 220.368 kb on - strand, within tolQat 220.368 kb on - strand, within tolQat 220.443 kb on + strand, within tolQat 220.603 kb on - strand, within tolQat 220.603 kb on - strand, within tolQat 220.604 kb on + strand, within tolQat 220.604 kb on + strand, within tolQat 220.780 kb on + strand, within tolRat 220.796 kb on - strand, within tolRat 220.944 kb on + strand, within tolRat 220.944 kb on + strand, within tolRat 220.945 kb on - strand, within tolRat 220.945 kb on - strand, within tolRat 221.074 kb on - strand, within tolRat 221.087 kb on + strand, within tolRat 221.104 kb on + strand, within tolRat 221.151 kb on + strandat 221.151 kb on + strandat 221.291 kb on + strandat 221.330 kb on + strandat 221.397 kb on - strand, within tolAat 222.153 kb on + strand, within tolAat 222.153 kb on + strand, within tolA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas67
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219,804 - ybgC HEPCGN_01445 0.43 -0.8
219,808 - ybgC HEPCGN_01445 0.44 -1.4
219,827 - ybgC HEPCGN_01445 0.49 +1.1
219,872 + ybgC HEPCGN_01445 0.60 +0.4
219,912 + ybgC HEPCGN_01445 0.70 -0.1
219,913 - ybgC HEPCGN_01445 0.70 -0.5
219,930 + ybgC HEPCGN_01445 0.75 -0.3
220,009 - +0.0
220,043 + +0.9
220,044 - -0.1
220,224 + tolQ HEPCGN_01450 0.28 +0.0
220,367 + tolQ HEPCGN_01450 0.49 +1.2
220,368 - tolQ HEPCGN_01450 0.49 +0.6
220,368 - tolQ HEPCGN_01450 0.49 -0.8
220,443 + tolQ HEPCGN_01450 0.60 -1.0
220,603 - tolQ HEPCGN_01450 0.83 -1.1
220,603 - tolQ HEPCGN_01450 0.83 -0.3
220,604 + tolQ HEPCGN_01450 0.83 -0.1
220,604 + tolQ HEPCGN_01450 0.83 -1.3
220,780 + tolR HEPCGN_01455 0.13 -0.5
220,796 - tolR HEPCGN_01455 0.17 -0.8
220,944 + tolR HEPCGN_01455 0.51 +1.9
220,944 + tolR HEPCGN_01455 0.51 +0.3
220,945 - tolR HEPCGN_01455 0.51 -0.1
220,945 - tolR HEPCGN_01455 0.51 -0.1
221,074 - tolR HEPCGN_01455 0.81 +0.3
221,087 + tolR HEPCGN_01455 0.84 -1.6
221,104 + tolR HEPCGN_01455 0.88 +1.9
221,151 + +0.5
221,151 + -0.9
221,291 + -0.4
221,330 + +1.1
221,397 - tolA HEPCGN_01460 0.15 +0.3
222,153 + tolA HEPCGN_01460 0.79 -0.1
222,153 + tolA HEPCGN_01460 0.79 -3.3

Or see this region's nucleotide sequence