Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cydB and cydX are separated by 14 nucleotides cydX and ybgE overlap by 1 nucleotides ybgE and ybgC are separated by 149 nucleotides ybgC and tolQ overlap by 4 nucleotides tolQ and tolR are separated by 3 nucleotides tolR and tolA are separated by 64 nucleotides
HEPCGN_01430: cydB - cytochrome d ubiquinol oxidase subunit II, at 217,918 to 219,057
cydB
HEPCGN_01435: cydX - cytochrome bd-I oxidase subunit CydX, at 219,072 to 219,185
cydX
HEPCGN_01440: ybgE - cyd operon protein YbgE, at 219,185 to 219,478
ybgE
HEPCGN_01445: ybgC - tol-pal system-associated acyl-CoA thioesterase, at 219,628 to 220,032
ybgC
HEPCGN_01450: tolQ - Tol-Pal system protein TolQ, at 220,029 to 220,721
tolQ
HEPCGN_01455: tolR - colicin uptake protein TolR, at 220,725 to 221,153
tolR
HEPCGN_01460: tolA - cell envelope integrity protein TolA, at 221,218 to 222,405
tolA
Position (kb) 220
221 Strain fitness (log2 ratio) -2
-1
0
1
2 at 219.055 kb on + strand at 219.059 kb on + strand at 219.196 kb on + strand at 219.210 kb on - strand at 219.522 kb on - strand at 219.575 kb on + strand at 219.576 kb on - strand at 219.581 kb on - strand at 219.581 kb on - strand at 219.581 kb on - strand at 219.591 kb on - strand at 219.624 kb on + strand at 219.624 kb on + strand at 219.624 kb on + strand at 219.633 kb on + strand at 219.634 kb on - strand at 219.664 kb on + strand at 219.667 kb on + strand at 219.667 kb on + strand at 219.667 kb on + strand at 219.668 kb on - strand at 219.676 kb on - strand, within ybgC at 219.709 kb on + strand, within ybgC at 219.804 kb on - strand, within ybgC at 219.808 kb on - strand, within ybgC at 219.827 kb on - strand, within ybgC at 219.872 kb on + strand, within ybgC at 219.912 kb on + strand, within ybgC at 219.913 kb on - strand, within ybgC at 219.930 kb on + strand, within ybgC at 220.009 kb on - strand at 220.043 kb on + strand at 220.044 kb on - strand at 220.224 kb on + strand, within tolQ at 220.367 kb on + strand, within tolQ at 220.368 kb on - strand, within tolQ at 220.368 kb on - strand, within tolQ at 220.443 kb on + strand, within tolQ at 220.603 kb on - strand, within tolQ at 220.603 kb on - strand, within tolQ at 220.604 kb on + strand, within tolQ at 220.604 kb on + strand, within tolQ at 220.780 kb on + strand, within tolR at 220.796 kb on - strand, within tolR at 220.944 kb on + strand, within tolR at 220.944 kb on + strand, within tolR at 220.945 kb on - strand, within tolR at 220.945 kb on - strand, within tolR at 221.074 kb on - strand, within tolR at 221.087 kb on + strand, within tolR at 221.104 kb on + strand, within tolR at 221.151 kb on + strand at 221.151 kb on + strand at 221.291 kb on + strand at 221.330 kb on + strand at 221.397 kb on - strand, within tolA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 219,055 + -0.4 219,059 + +0.9 219,196 + +0.1 219,210 - -1.7 219,522 - +1.0 219,575 + -0.7 219,576 - +1.3 219,581 - -0.3 219,581 - +0.7 219,581 - -0.5 219,591 - -0.1 219,624 + +0.4 219,624 + +0.1 219,624 + -0.1 219,633 + -1.0 219,634 - -1.8 219,664 + +0.5 219,667 + -1.3 219,667 + -0.1 219,667 + +0.4 219,668 - +0.0 219,676 - ybgC HEPCGN_01445 0.12 -0.5 219,709 + ybgC HEPCGN_01445 0.20 +0.6 219,804 - ybgC HEPCGN_01445 0.43 -0.8 219,808 - ybgC HEPCGN_01445 0.44 -1.4 219,827 - ybgC HEPCGN_01445 0.49 +1.1 219,872 + ybgC HEPCGN_01445 0.60 +0.4 219,912 + ybgC HEPCGN_01445 0.70 -0.1 219,913 - ybgC HEPCGN_01445 0.70 -0.5 219,930 + ybgC HEPCGN_01445 0.75 -0.3 220,009 - +0.0 220,043 + +0.9 220,044 - -0.1 220,224 + tolQ HEPCGN_01450 0.28 +0.0 220,367 + tolQ HEPCGN_01450 0.49 +1.2 220,368 - tolQ HEPCGN_01450 0.49 +0.6 220,368 - tolQ HEPCGN_01450 0.49 -0.8 220,443 + tolQ HEPCGN_01450 0.60 -1.0 220,603 - tolQ HEPCGN_01450 0.83 -1.1 220,603 - tolQ HEPCGN_01450 0.83 -0.3 220,604 + tolQ HEPCGN_01450 0.83 -0.1 220,604 + tolQ HEPCGN_01450 0.83 -1.3 220,780 + tolR HEPCGN_01455 0.13 -0.5 220,796 - tolR HEPCGN_01455 0.17 -0.8 220,944 + tolR HEPCGN_01455 0.51 +1.9 220,944 + tolR HEPCGN_01455 0.51 +0.3 220,945 - tolR HEPCGN_01455 0.51 -0.1 220,945 - tolR HEPCGN_01455 0.51 -0.1 221,074 - tolR HEPCGN_01455 0.81 +0.3 221,087 + tolR HEPCGN_01455 0.84 -1.6 221,104 + tolR HEPCGN_01455 0.88 +1.9 221,151 + +0.5 221,151 + -0.9 221,291 + -0.4 221,330 + +1.1 221,397 - tolA HEPCGN_01460 0.15 +0.3
Or see this region's nucleotide sequence