Experiment: Bas67
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdG and nfsB are separated by 107 nucleotides nfsB and ybdF are separated by 78 nucleotides ybdF and ybdJ are separated by 64 nucleotides ybdJ and ybdK are separated by 65 nucleotides
HEPCGN_00615: ybdG - mechanosensitive ion channel YbdG, at 50,078 to 51,325
ybdG
HEPCGN_00620: nfsB - oxygen-insensitive NAD(P)H nitroreductase, at 51,433 to 52,086
nfsB
HEPCGN_00625: ybdF - MmcQ/YjbR family DNA-binding protein, at 52,165 to 52,548
ybdF
HEPCGN_00630: ybdJ - Uncharacterized protein YbdJ, at 52,613 to 52,861
ybdJ
HEPCGN_00635: ybdK - YbdK family carboxylate-amine ligase, at 52,927 to 54,045
ybdK
Position (kb) 51
52
53 Strain fitness (log2 ratio) -2
-1
0
1
2 at 50.441 kb on + strand, within ybdG at 50.442 kb on - strand, within ybdG at 50.445 kb on + strand, within ybdG at 50.445 kb on + strand, within ybdG at 50.468 kb on - strand, within ybdG at 50.506 kb on + strand, within ybdG at 50.521 kb on + strand, within ybdG at 50.522 kb on - strand, within ybdG at 50.537 kb on - strand, within ybdG at 50.540 kb on - strand, within ybdG at 50.612 kb on - strand, within ybdG at 50.623 kb on + strand, within ybdG at 50.624 kb on - strand, within ybdG at 50.624 kb on - strand, within ybdG at 50.689 kb on - strand, within ybdG at 50.746 kb on + strand, within ybdG at 50.747 kb on - strand, within ybdG at 50.776 kb on + strand, within ybdG at 50.997 kb on + strand, within ybdG at 50.997 kb on + strand, within ybdG at 50.997 kb on + strand, within ybdG at 50.998 kb on - strand, within ybdG at 51.034 kb on + strand, within ybdG at 51.034 kb on + strand, within ybdG at 51.034 kb on + strand, within ybdG at 51.111 kb on - strand, within ybdG at 51.339 kb on + strand at 51.339 kb on + strand at 51.340 kb on - strand at 51.340 kb on - strand at 51.340 kb on - strand at 51.353 kb on - strand at 51.434 kb on + strand at 51.656 kb on + strand, within nfsB at 51.885 kb on - strand, within nfsB at 51.980 kb on + strand, within nfsB at 51.980 kb on + strand, within nfsB at 51.981 kb on - strand, within nfsB at 52.048 kb on - strand at 52.166 kb on + strand at 52.175 kb on - strand at 52.212 kb on + strand, within ybdF at 52.314 kb on + strand, within ybdF at 52.348 kb on + strand, within ybdF at 52.349 kb on - strand, within ybdF at 52.349 kb on - strand, within ybdF at 52.349 kb on - strand, within ybdF at 52.428 kb on + strand, within ybdF at 52.428 kb on + strand, within ybdF at 52.543 kb on - strand at 52.584 kb on - strand at 52.584 kb on - strand at 52.645 kb on + strand, within ybdJ at 52.645 kb on + strand, within ybdJ at 52.663 kb on - strand, within ybdJ at 52.847 kb on + strand at 52.901 kb on + strand at 53.076 kb on + strand, within ybdK at 53.077 kb on - strand, within ybdK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas67 remove 50,441 + ybdG HEPCGN_00615 0.29 -0.5 50,442 - ybdG HEPCGN_00615 0.29 +0.1 50,445 + ybdG HEPCGN_00615 0.29 -1.3 50,445 + ybdG HEPCGN_00615 0.29 -1.7 50,468 - ybdG HEPCGN_00615 0.31 -0.9 50,506 + ybdG HEPCGN_00615 0.34 +0.3 50,521 + ybdG HEPCGN_00615 0.35 -0.0 50,522 - ybdG HEPCGN_00615 0.36 -0.7 50,537 - ybdG HEPCGN_00615 0.37 +1.1 50,540 - ybdG HEPCGN_00615 0.37 +0.3 50,612 - ybdG HEPCGN_00615 0.43 -0.2 50,623 + ybdG HEPCGN_00615 0.44 +0.4 50,624 - ybdG HEPCGN_00615 0.44 -1.9 50,624 - ybdG HEPCGN_00615 0.44 -2.0 50,689 - ybdG HEPCGN_00615 0.49 +2.6 50,746 + ybdG HEPCGN_00615 0.54 +1.5 50,747 - ybdG HEPCGN_00615 0.54 -0.0 50,776 + ybdG HEPCGN_00615 0.56 +1.1 50,997 + ybdG HEPCGN_00615 0.74 -0.4 50,997 + ybdG HEPCGN_00615 0.74 +0.3 50,997 + ybdG HEPCGN_00615 0.74 +0.9 50,998 - ybdG HEPCGN_00615 0.74 -0.7 51,034 + ybdG HEPCGN_00615 0.77 -0.6 51,034 + ybdG HEPCGN_00615 0.77 +1.0 51,034 + ybdG HEPCGN_00615 0.77 -0.0 51,111 - ybdG HEPCGN_00615 0.83 +0.2 51,339 + +0.5 51,339 + +1.0 51,340 - +0.2 51,340 - +0.7 51,340 - -0.0 51,353 - +0.3 51,434 + -2.3 51,656 + nfsB HEPCGN_00620 0.34 -1.4 51,885 - nfsB HEPCGN_00620 0.69 -0.3 51,980 + nfsB HEPCGN_00620 0.84 -1.2 51,980 + nfsB HEPCGN_00620 0.84 -0.4 51,981 - nfsB HEPCGN_00620 0.84 -0.8 52,048 - -1.0 52,166 + +0.0 52,175 - -1.5 52,212 + ybdF HEPCGN_00625 0.12 +0.4 52,314 + ybdF HEPCGN_00625 0.39 +0.0 52,348 + ybdF HEPCGN_00625 0.48 +1.1 52,349 - ybdF HEPCGN_00625 0.48 -1.6 52,349 - ybdF HEPCGN_00625 0.48 -0.1 52,349 - ybdF HEPCGN_00625 0.48 -2.0 52,428 + ybdF HEPCGN_00625 0.68 -1.1 52,428 + ybdF HEPCGN_00625 0.68 +1.2 52,543 - -0.4 52,584 - -0.8 52,584 - +0.4 52,645 + ybdJ HEPCGN_00630 0.13 +0.5 52,645 + ybdJ HEPCGN_00630 0.13 +0.3 52,663 - ybdJ HEPCGN_00630 0.20 +0.2 52,847 + -0.6 52,901 + -1.2 53,076 + ybdK HEPCGN_00635 0.13 +0.8 53,077 - ybdK HEPCGN_00635 0.13 +1.0
Or see this region's nucleotide sequence