Strain Fitness in Escherichia coli ECOR38 around HEPCGN_22500

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmqo and eco are separated by 149 nucleotideseco and yojO are separated by 134 nucleotidesyojO and HEPCGN_22505 are separated by 51 nucleotidesHEPCGN_22505 and napF are separated by 15 nucleotidesnapF and napD overlap by 11 nucleotidesnapD and napA overlap by 4 nucleotides HEPCGN_22490: mqo - malate dehydrogenase (quinone), at 3,271,706 to 3,273,352 mqo HEPCGN_22495: eco - serine protease inhibitor ecotin, at 3,273,502 to 3,273,990 eco HEPCGN_22500: yojO - Uncharacterized protein YojO, at 3,274,125 to 3,274,289 yojO HEPCGN_22505: HEPCGN_22505 - Orf 5, at 3,274,341 to 3,274,382 _22505 HEPCGN_22510: napF - ferredoxin-type protein NapF, at 3,274,398 to 3,274,892 napF HEPCGN_22515: napD - chaperone NapD, at 3,274,882 to 3,275,145 napD HEPCGN_22520: napA - nitrate reductase catalytic subunit NapA, at 3,275,142 to 3,277,628 napA Position (kb) 3274 3275Strain fitness (log2 ratio) -2 -1 0 1 2at 3273.186 kb on - strand, within mqoat 3273.210 kb on + strandat 3273.286 kb on + strandat 3273.287 kb on - strandat 3273.287 kb on - strandat 3273.287 kb on - strandat 3273.289 kb on + strandat 3273.290 kb on - strandat 3273.350 kb on + strandat 3273.350 kb on + strandat 3273.350 kb on + strandat 3273.350 kb on + strandat 3273.350 kb on + strandat 3273.350 kb on + strandat 3273.351 kb on - strandat 3273.351 kb on - strandat 3273.351 kb on - strandat 3273.400 kb on - strandat 3273.498 kb on + strandat 3273.498 kb on + strandat 3273.499 kb on - strandat 3273.499 kb on - strandat 3273.551 kb on + strand, within ecoat 3273.577 kb on - strand, within ecoat 3273.603 kb on + strand, within ecoat 3273.606 kb on - strand, within ecoat 3273.717 kb on - strand, within ecoat 3273.720 kb on - strand, within ecoat 3273.809 kb on - strand, within ecoat 3273.817 kb on + strand, within ecoat 3273.829 kb on - strand, within ecoat 3273.893 kb on + strand, within ecoat 3273.893 kb on + strand, within ecoat 3273.894 kb on - strand, within ecoat 3273.976 kb on + strandat 3273.977 kb on - strandat 3274.051 kb on - strandat 3274.082 kb on + strandat 3274.082 kb on + strandat 3274.083 kb on - strandat 3274.155 kb on - strand, within yojOat 3274.155 kb on - strand, within yojOat 3274.159 kb on - strand, within yojOat 3274.269 kb on + strand, within yojOat 3274.305 kb on + strandat 3274.309 kb on - strandat 3274.380 kb on + strandat 3274.381 kb on - strandat 3274.384 kb on + strandat 3274.385 kb on - strandat 3274.427 kb on - strandat 3274.604 kb on + strand, within napFat 3274.764 kb on - strand, within napFat 3274.805 kb on + strand, within napFat 3274.806 kb on - strand, within napFat 3274.864 kb on + strandat 3274.865 kb on - strandat 3274.865 kb on - strandat 3274.875 kb on + strandat 3274.890 kb on + strandat 3274.890 kb on + strandat 3274.890 kb on + strandat 3275.069 kb on + strand, within napDat 3275.070 kb on - strand, within napDat 3275.258 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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3,273,186 - mqo HEPCGN_22490 0.90 +0.5
3,273,210 + +0.0
3,273,286 + -0.3
3,273,287 - +0.1
3,273,287 - +0.1
3,273,287 - +0.6
3,273,289 + +0.2
3,273,290 - -1.0
3,273,350 + +0.3
3,273,350 + -0.2
3,273,350 + -0.1
3,273,350 + -0.5
3,273,350 + -1.9
3,273,350 + +0.7
3,273,351 - +0.9
3,273,351 - +2.1
3,273,351 - -1.2
3,273,400 - +0.2
3,273,498 + +0.5
3,273,498 + +0.1
3,273,499 - -0.8
3,273,499 - +0.2
3,273,551 + eco HEPCGN_22495 0.10 +0.2
3,273,577 - eco HEPCGN_22495 0.15 -0.8
3,273,603 + eco HEPCGN_22495 0.21 +1.6
3,273,606 - eco HEPCGN_22495 0.21 -0.1
3,273,717 - eco HEPCGN_22495 0.44 +1.4
3,273,720 - eco HEPCGN_22495 0.45 -1.1
3,273,809 - eco HEPCGN_22495 0.63 -0.1
3,273,817 + eco HEPCGN_22495 0.64 +0.4
3,273,829 - eco HEPCGN_22495 0.67 -0.3
3,273,893 + eco HEPCGN_22495 0.80 +0.1
3,273,893 + eco HEPCGN_22495 0.80 +0.3
3,273,894 - eco HEPCGN_22495 0.80 +1.4
3,273,976 + +0.8
3,273,977 - +0.1
3,274,051 - -0.0
3,274,082 + +1.1
3,274,082 + +0.8
3,274,083 - -0.7
3,274,155 - yojO HEPCGN_22500 0.18 -0.1
3,274,155 - yojO HEPCGN_22500 0.18 -1.7
3,274,159 - yojO HEPCGN_22500 0.21 -0.7
3,274,269 + yojO HEPCGN_22500 0.87 -1.3
3,274,305 + +0.3
3,274,309 - -0.2
3,274,380 + -0.6
3,274,381 - -0.9
3,274,384 + -0.6
3,274,385 - -1.5
3,274,427 - +0.4
3,274,604 + napF HEPCGN_22510 0.42 -0.6
3,274,764 - napF HEPCGN_22510 0.74 +1.6
3,274,805 + napF HEPCGN_22510 0.82 -0.2
3,274,806 - napF HEPCGN_22510 0.82 -0.3
3,274,864 + +0.6
3,274,865 - +1.9
3,274,865 - +0.8
3,274,875 + -0.4
3,274,890 + +0.5
3,274,890 + +0.2
3,274,890 + +1.0
3,275,069 + napD HEPCGN_22515 0.71 +0.1
3,275,070 - napD HEPCGN_22515 0.71 +1.9
3,275,258 + +0.1

Or see this region's nucleotide sequence