Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14540

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaC and rfaF are separated by 3 nucleotidesrfaF and rfaD are separated by 9 nucleotides HEPCGN_14535: rfaC - lipopolysaccharide heptosyltransferase RfaC, at 1,660,450 to 1,661,421 rfaC HEPCGN_14540: rfaF - ADP-heptose--LPS heptosyltransferase RfaF, at 1,661,425 to 1,662,471 rfaF HEPCGN_14545: rfaD - ADP-glyceromanno-heptose 6-epimerase, at 1,662,481 to 1,663,413 rfaD Position (kb) 1661 1662 1663Strain fitness (log2 ratio) -2 -1 0 1at 1660.564 kb on + strand, within rfaCat 1661.441 kb on - strandat 1661.874 kb on - strand, within rfaFat 1661.874 kb on - strand, within rfaFat 1661.874 kb on - strand, within rfaFat 1661.965 kb on - strand, within rfaFat 1662.039 kb on + strand, within rfaFat 1662.341 kb on - strand, within rfaFat 1663.462 kb on - strandat 1663.462 kb on - strandat 1663.468 kb on - strandat 1663.468 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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1,660,564 + rfaC HEPCGN_14535 0.12 +0.7
1,661,441 - +0.4
1,661,874 - rfaF HEPCGN_14540 0.43 -2.0
1,661,874 - rfaF HEPCGN_14540 0.43 +1.4
1,661,874 - rfaF HEPCGN_14540 0.43 +0.7
1,661,965 - rfaF HEPCGN_14540 0.52 -0.5
1,662,039 + rfaF HEPCGN_14540 0.59 +0.3
1,662,341 - rfaF HEPCGN_14540 0.87 -1.3
1,663,462 - -0.2
1,663,462 - +0.1
1,663,468 - -0.7
1,663,468 - +0.1

Or see this region's nucleotide sequence