Experiment: TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt waaO and rfaJ are separated by 16 nucleotides rfaJ and HEPCGN_14500 are separated by 9 nucleotides HEPCGN_14500 and HEPCGN_14510 are separated by 103 nucleotides
HEPCGN_14490: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,652,897 to 1,653,913
waaO
HEPCGN_14495: rfaJ - lipopolysaccharide 1,2-glucosyltransferase, at 1,653,930 to 1,654,925
rfaJ
HEPCGN_14500: HEPCGN_14500 - lipopolysaccharide core heptose(II) kinase RfaY, at 1,654,935 to 1,655,099
_14500
HEPCGN_14510: HEPCGN_14510 - IS4-like element ISVsa5 family transposase, at 1,655,203 to 1,656,411
_14510
Position (kb)
1653
1654
1655 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1653.058 kb on + strand, within waaO at 1653.146 kb on + strand, within waaO at 1653.152 kb on - strand, within waaO at 1653.230 kb on + strand, within waaO at 1653.275 kb on - strand, within waaO at 1653.349 kb on + strand, within waaO at 1653.418 kb on + strand, within waaO at 1653.460 kb on - strand, within waaO at 1653.463 kb on + strand, within waaO at 1653.471 kb on + strand, within waaO at 1653.472 kb on - strand, within waaO at 1653.549 kb on - strand, within waaO at 1653.752 kb on - strand, within waaO at 1654.178 kb on - strand, within rfaJ at 1654.297 kb on - strand, within rfaJ at 1654.391 kb on + strand, within rfaJ at 1654.394 kb on + strand, within rfaJ at 1654.513 kb on + strand, within rfaJ at 1654.661 kb on + strand, within rfaJ at 1654.674 kb on + strand, within rfaJ at 1654.723 kb on + strand, within rfaJ at 1654.740 kb on - strand, within rfaJ at 1654.772 kb on + strand, within rfaJ at 1654.806 kb on + strand, within rfaJ at 1654.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TP12 remove 1,653,058 + waaO HEPCGN_14490 0.16 -2.3 1,653,146 + waaO HEPCGN_14490 0.24 +0.2 1,653,152 - waaO HEPCGN_14490 0.25 -2.1 1,653,230 + waaO HEPCGN_14490 0.33 -2.6 1,653,275 - waaO HEPCGN_14490 0.37 -3.2 1,653,349 + waaO HEPCGN_14490 0.44 +0.4 1,653,418 + waaO HEPCGN_14490 0.51 -1.2 1,653,460 - waaO HEPCGN_14490 0.55 +3.0 1,653,463 + waaO HEPCGN_14490 0.56 -1.1 1,653,471 + waaO HEPCGN_14490 0.56 -1.5 1,653,472 - waaO HEPCGN_14490 0.57 +1.6 1,653,549 - waaO HEPCGN_14490 0.64 -2.0 1,653,752 - waaO HEPCGN_14490 0.84 -1.9 1,654,178 - rfaJ HEPCGN_14495 0.25 -1.2 1,654,297 - rfaJ HEPCGN_14495 0.37 -3.7 1,654,391 + rfaJ HEPCGN_14495 0.46 -2.7 1,654,394 + rfaJ HEPCGN_14495 0.47 -2.5 1,654,513 + rfaJ HEPCGN_14495 0.59 -2.4 1,654,661 + rfaJ HEPCGN_14495 0.73 -3.0 1,654,674 + rfaJ HEPCGN_14495 0.75 -1.0 1,654,723 + rfaJ HEPCGN_14495 0.80 -1.8 1,654,740 - rfaJ HEPCGN_14495 0.81 -2.6 1,654,772 + rfaJ HEPCGN_14495 0.85 -2.3 1,654,806 + rfaJ HEPCGN_14495 0.88 -1.0 1,654,854 - -3.8
Or see this region's nucleotide sequence