Strain Fitness in Escherichia coli ECOR38 around HEPCGN_14490

Experiment: TP12

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaG and rfaP overlap by 8 nucleotidesrfaP and waaO are separated by 15 nucleotideswaaO and rfaJ are separated by 16 nucleotides HEPCGN_14480: rfaG - Lipopolysaccharide core biosynthesis protein RfaG, at 1,650,967 to 1,652,091 rfaG HEPCGN_14485: rfaP - lipopolysaccharide core heptose(I) kinase RfaP, at 1,652,084 to 1,652,881 rfaP HEPCGN_14490: waaO - lipopolysaccharide 3-alpha-galactosyltransferase, at 1,652,897 to 1,653,913 waaO HEPCGN_14495: rfaJ - lipopolysaccharide 1,2-glucosyltransferase, at 1,653,930 to 1,654,925 rfaJ Position (kb) 1652 1653 1654Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1652.026 kb on - strandat 1652.036 kb on + strandat 1652.037 kb on - strandat 1652.126 kb on - strandat 1652.262 kb on + strand, within rfaPat 1652.262 kb on + strand, within rfaPat 1652.354 kb on - strand, within rfaPat 1652.356 kb on + strand, within rfaPat 1652.356 kb on + strand, within rfaPat 1652.356 kb on + strand, within rfaPat 1652.356 kb on + strand, within rfaPat 1652.357 kb on - strand, within rfaPat 1652.360 kb on + strand, within rfaPat 1652.361 kb on - strand, within rfaPat 1652.361 kb on - strand, within rfaPat 1652.457 kb on - strand, within rfaPat 1652.503 kb on + strand, within rfaPat 1652.512 kb on + strand, within rfaPat 1652.574 kb on - strand, within rfaPat 1652.574 kb on - strand, within rfaPat 1652.576 kb on - strand, within rfaPat 1652.701 kb on + strand, within rfaPat 1652.761 kb on + strand, within rfaPat 1652.771 kb on + strand, within rfaPat 1653.058 kb on + strand, within waaOat 1653.146 kb on + strand, within waaOat 1653.152 kb on - strand, within waaOat 1653.230 kb on + strand, within waaOat 1653.275 kb on - strand, within waaOat 1653.349 kb on + strand, within waaOat 1653.418 kb on + strand, within waaOat 1653.460 kb on - strand, within waaOat 1653.463 kb on + strand, within waaOat 1653.471 kb on + strand, within waaOat 1653.472 kb on - strand, within waaOat 1653.549 kb on - strand, within waaOat 1653.752 kb on - strand, within waaOat 1654.178 kb on - strand, within rfaJat 1654.297 kb on - strand, within rfaJat 1654.391 kb on + strand, within rfaJat 1654.394 kb on + strand, within rfaJat 1654.513 kb on + strand, within rfaJat 1654.661 kb on + strand, within rfaJat 1654.674 kb on + strand, within rfaJat 1654.723 kb on + strand, within rfaJat 1654.740 kb on - strand, within rfaJat 1654.772 kb on + strand, within rfaJat 1654.806 kb on + strand, within rfaJat 1654.854 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP12
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1,652,026 - -1.6
1,652,036 + +0.5
1,652,037 - +1.5
1,652,126 - +1.1
1,652,262 + rfaP HEPCGN_14485 0.22 +0.2
1,652,262 + rfaP HEPCGN_14485 0.22 -0.1
1,652,354 - rfaP HEPCGN_14485 0.34 -1.3
1,652,356 + rfaP HEPCGN_14485 0.34 +0.5
1,652,356 + rfaP HEPCGN_14485 0.34 +0.5
1,652,356 + rfaP HEPCGN_14485 0.34 -1.4
1,652,356 + rfaP HEPCGN_14485 0.34 -0.5
1,652,357 - rfaP HEPCGN_14485 0.34 -2.1
1,652,360 + rfaP HEPCGN_14485 0.35 +1.5
1,652,361 - rfaP HEPCGN_14485 0.35 -1.2
1,652,361 - rfaP HEPCGN_14485 0.35 +0.7
1,652,457 - rfaP HEPCGN_14485 0.47 +3.0
1,652,503 + rfaP HEPCGN_14485 0.53 -0.1
1,652,512 + rfaP HEPCGN_14485 0.54 -0.7
1,652,574 - rfaP HEPCGN_14485 0.61 -2.8
1,652,574 - rfaP HEPCGN_14485 0.61 -1.4
1,652,576 - rfaP HEPCGN_14485 0.62 +0.2
1,652,701 + rfaP HEPCGN_14485 0.77 +1.1
1,652,761 + rfaP HEPCGN_14485 0.85 +1.3
1,652,771 + rfaP HEPCGN_14485 0.86 +0.1
1,653,058 + waaO HEPCGN_14490 0.16 -2.3
1,653,146 + waaO HEPCGN_14490 0.24 +0.2
1,653,152 - waaO HEPCGN_14490 0.25 -2.1
1,653,230 + waaO HEPCGN_14490 0.33 -2.6
1,653,275 - waaO HEPCGN_14490 0.37 -3.2
1,653,349 + waaO HEPCGN_14490 0.44 +0.4
1,653,418 + waaO HEPCGN_14490 0.51 -1.2
1,653,460 - waaO HEPCGN_14490 0.55 +3.0
1,653,463 + waaO HEPCGN_14490 0.56 -1.1
1,653,471 + waaO HEPCGN_14490 0.56 -1.5
1,653,472 - waaO HEPCGN_14490 0.57 +1.6
1,653,549 - waaO HEPCGN_14490 0.64 -2.0
1,653,752 - waaO HEPCGN_14490 0.84 -1.9
1,654,178 - rfaJ HEPCGN_14495 0.25 -1.2
1,654,297 - rfaJ HEPCGN_14495 0.37 -3.7
1,654,391 + rfaJ HEPCGN_14495 0.46 -2.7
1,654,394 + rfaJ HEPCGN_14495 0.47 -2.5
1,654,513 + rfaJ HEPCGN_14495 0.59 -2.4
1,654,661 + rfaJ HEPCGN_14495 0.73 -3.0
1,654,674 + rfaJ HEPCGN_14495 0.75 -1.0
1,654,723 + rfaJ HEPCGN_14495 0.80 -1.8
1,654,740 - rfaJ HEPCGN_14495 0.81 -2.6
1,654,772 + rfaJ HEPCGN_14495 0.85 -2.3
1,654,806 + rfaJ HEPCGN_14495 0.88 -1.0
1,654,854 - -3.8

Or see this region's nucleotide sequence