Experiment: TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sdhA and sdhB are separated by 15 nucleotides sdhB and sucA are separated by 200 nucleotides sucA and odhB are separated by 14 nucleotides
HEPCGN_01295: sdhA - succinate dehydrogenase flavoprotein subunit, at 183,425 to 185,191
sdhA
HEPCGN_01300: sdhB - succinate dehydrogenase iron-sulfur subunit SdhB, at 185,207 to 185,923
sdhB
HEPCGN_01305: sucA - 2-oxoglutarate dehydrogenase E1 component, at 186,124 to 188,925
sucA
HEPCGN_01310: odhB - 2-oxoglutarate dehydrogenase complex dihydrolipoyllysine-residue succinyltransferase, at 188,940 to 190,157
odhB
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 185.186 kb on + strand at 185.186 kb on + strand at 185.186 kb on + strand at 185.186 kb on + strand at 185.186 kb on + strand at 185.186 kb on + strand at 185.187 kb on - strand at 185.235 kb on - strand at 185.264 kb on - strand at 185.273 kb on + strand at 185.274 kb on - strand at 185.323 kb on + strand, within sdhB at 185.431 kb on + strand, within sdhB at 185.432 kb on - strand, within sdhB at 185.434 kb on + strand, within sdhB at 185.434 kb on + strand, within sdhB at 185.521 kb on - strand, within sdhB at 185.521 kb on - strand, within sdhB at 185.564 kb on + strand, within sdhB at 185.565 kb on - strand, within sdhB at 185.575 kb on + strand, within sdhB at 185.608 kb on - strand, within sdhB at 185.608 kb on - strand, within sdhB at 185.646 kb on - strand, within sdhB at 185.646 kb on - strand, within sdhB at 185.646 kb on - strand, within sdhB at 185.653 kb on + strand, within sdhB at 185.654 kb on - strand, within sdhB at 185.654 kb on - strand, within sdhB at 185.654 kb on - strand, within sdhB at 185.851 kb on - strand, within sdhB at 185.926 kb on - strand at 185.989 kb on + strand at 185.990 kb on - strand at 186.001 kb on + strand at 186.099 kb on + strand at 186.099 kb on + strand at 186.112 kb on + strand at 186.191 kb on + strand at 186.613 kb on + strand, within sucA at 186.639 kb on + strand, within sucA at 186.639 kb on + strand, within sucA at 186.639 kb on + strand, within sucA at 186.639 kb on + strand, within sucA at 186.640 kb on - strand, within sucA at 186.640 kb on - strand, within sucA at 186.982 kb on - strand, within sucA at 187.109 kb on + strand, within sucA at 187.110 kb on - strand, within sucA at 187.274 kb on + strand, within sucA at 187.278 kb on + strand, within sucA at 187.278 kb on + strand, within sucA at 187.278 kb on + strand, within sucA at 187.343 kb on - strand, within sucA at 187.343 kb on - strand, within sucA at 187.446 kb on + strand, within sucA at 187.456 kb on - strand, within sucA at 187.582 kb on + strand, within sucA at 187.582 kb on + strand, within sucA at 188.096 kb on + strand, within sucA at 188.097 kb on - strand, within sucA at 188.128 kb on + strand, within sucA at 188.129 kb on - strand, within sucA at 188.135 kb on - strand, within sucA at 188.135 kb on - strand, within sucA at 188.163 kb on + strand, within sucA at 188.927 kb on + strand at 188.927 kb on + strand at 188.934 kb on + strand at 188.950 kb on + strand at 188.954 kb on + strand at 188.954 kb on + strand at 189.034 kb on + strand at 189.034 kb on + strand at 189.034 kb on + strand at 189.134 kb on + strand, within odhB at 189.314 kb on + strand, within odhB at 189.335 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.641 kb on + strand, within odhB at 189.726 kb on + strand, within odhB at 189.735 kb on + strand, within odhB at 189.851 kb on + strand, within odhB
Per-strain Table
Position Strand Gene LocusTag Fraction TP12 remove 185,186 + -2.0 185,186 + -1.5 185,186 + -0.1 185,186 + +0.2 185,186 + -1.4 185,186 + -1.4 185,187 - -0.1 185,235 - +0.5 185,264 - +0.9 185,273 + -2.2 185,274 - +1.1 185,323 + sdhB HEPCGN_01300 0.16 -1.2 185,431 + sdhB HEPCGN_01300 0.31 -0.2 185,432 - sdhB HEPCGN_01300 0.31 -1.7 185,434 + sdhB HEPCGN_01300 0.32 -0.7 185,434 + sdhB HEPCGN_01300 0.32 -0.9 185,521 - sdhB HEPCGN_01300 0.44 -0.4 185,521 - sdhB HEPCGN_01300 0.44 -0.3 185,564 + sdhB HEPCGN_01300 0.50 -0.4 185,565 - sdhB HEPCGN_01300 0.50 -1.1 185,575 + sdhB HEPCGN_01300 0.51 -0.4 185,608 - sdhB HEPCGN_01300 0.56 -0.8 185,608 - sdhB HEPCGN_01300 0.56 -1.0 185,646 - sdhB HEPCGN_01300 0.61 +1.5 185,646 - sdhB HEPCGN_01300 0.61 -0.1 185,646 - sdhB HEPCGN_01300 0.61 -1.2 185,653 + sdhB HEPCGN_01300 0.62 -1.5 185,654 - sdhB HEPCGN_01300 0.62 +0.4 185,654 - sdhB HEPCGN_01300 0.62 +0.4 185,654 - sdhB HEPCGN_01300 0.62 +0.6 185,851 - sdhB HEPCGN_01300 0.90 -1.6 185,926 - -0.2 185,989 + +1.1 185,990 - +0.4 186,001 + -0.4 186,099 + -0.7 186,099 + -1.3 186,112 + -2.4 186,191 + +1.1 186,613 + sucA HEPCGN_01305 0.17 -2.1 186,639 + sucA HEPCGN_01305 0.18 -2.7 186,639 + sucA HEPCGN_01305 0.18 -3.0 186,639 + sucA HEPCGN_01305 0.18 -2.3 186,639 + sucA HEPCGN_01305 0.18 -3.3 186,640 - sucA HEPCGN_01305 0.18 -0.9 186,640 - sucA HEPCGN_01305 0.18 -1.4 186,982 - sucA HEPCGN_01305 0.31 -1.5 187,109 + sucA HEPCGN_01305 0.35 -2.6 187,110 - sucA HEPCGN_01305 0.35 -0.8 187,274 + sucA HEPCGN_01305 0.41 -0.9 187,278 + sucA HEPCGN_01305 0.41 -1.0 187,278 + sucA HEPCGN_01305 0.41 -0.2 187,278 + sucA HEPCGN_01305 0.41 +0.5 187,343 - sucA HEPCGN_01305 0.44 -0.4 187,343 - sucA HEPCGN_01305 0.44 -1.9 187,446 + sucA HEPCGN_01305 0.47 +0.5 187,456 - sucA HEPCGN_01305 0.48 -1.1 187,582 + sucA HEPCGN_01305 0.52 +1.4 187,582 + sucA HEPCGN_01305 0.52 +0.0 188,096 + sucA HEPCGN_01305 0.70 +0.7 188,097 - sucA HEPCGN_01305 0.70 -2.3 188,128 + sucA HEPCGN_01305 0.72 +0.4 188,129 - sucA HEPCGN_01305 0.72 -1.2 188,135 - sucA HEPCGN_01305 0.72 +0.4 188,135 - sucA HEPCGN_01305 0.72 -2.8 188,163 + sucA HEPCGN_01305 0.73 +0.2 188,927 + -0.7 188,927 + -0.8 188,934 + +1.2 188,950 + +0.3 188,954 + +1.8 188,954 + +0.3 189,034 + +0.7 189,034 + +0.9 189,034 + -0.8 189,134 + odhB HEPCGN_01310 0.16 +0.3 189,314 + odhB HEPCGN_01310 0.31 +0.6 189,335 + odhB HEPCGN_01310 0.32 +2.4 189,641 + odhB HEPCGN_01310 0.58 +1.0 189,641 + odhB HEPCGN_01310 0.58 +0.1 189,641 + odhB HEPCGN_01310 0.58 +0.6 189,726 + odhB HEPCGN_01310 0.65 +0.0 189,735 + odhB HEPCGN_01310 0.65 +1.4 189,851 + odhB HEPCGN_01310 0.75 +2.5
Or see this region's nucleotide sequence