Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hmoA and HEPCGN_10830 overlap by 8 nucleotides HEPCGN_10830 and arsR are separated by 70 nucleotides arsR and tetR(B overlap by 23 nucleotides tetR(B and tet(B are separated by 81 nucleotides
HEPCGN_10825: hmoA - antibiotic biosynthesis monooxygenase, at 908,626 to 908,946
hmoA
HEPCGN_10830: HEPCGN_10830 - amino acid-binding protein, at 908,939 to 909,325
_10830
HEPCGN_10835: arsR - transcriptional regulator, at 909,396 to 910,019
arsR
HEPCGN_10840: tetR(B - tetracycline resistance transcriptional repressor TetR(B), at 909,997 to 910,620
tetR(B
HEPCGN_10845: tet(B - tetracycline efflux MFS transporter Tet(B), at 910,702 to 911,907
tet(B
Position (kb) 909
910
911 Strain fitness (log2 ratio) -1
0
1 at 908.678 kb on - strand, within hmoA at 909.003 kb on + strand, within HEPCGN_10830 at 909.044 kb on - strand, within HEPCGN_10830 at 909.129 kb on + strand, within HEPCGN_10830 at 909.482 kb on + strand, within arsR at 909.485 kb on - strand, within arsR at 909.700 kb on + strand, within arsR at 910.135 kb on - strand, within tetR(B at 910.227 kb on - strand, within tetR(B at 910.504 kb on - strand, within tetR(B at 910.504 kb on - strand, within tetR(B at 910.520 kb on - strand, within tetR(B at 910.520 kb on - strand, within tetR(B at 910.520 kb on - strand, within tetR(B at 910.609 kb on + strand at 910.735 kb on - strand at 910.934 kb on + strand, within tet(B at 910.959 kb on - strand, within tet(B
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 908,678 - hmoA HEPCGN_10825 0.16 +0.1 909,003 + HEPCGN_10830 0.17 +1.0 909,044 - HEPCGN_10830 0.27 -0.2 909,129 + HEPCGN_10830 0.49 -1.1 909,482 + arsR HEPCGN_10835 0.14 -0.6 909,485 - arsR HEPCGN_10835 0.14 -1.5 909,700 + arsR HEPCGN_10835 0.49 -0.7 910,135 - tetR(B HEPCGN_10840 0.22 -0.0 910,227 - tetR(B HEPCGN_10840 0.37 +0.4 910,504 - tetR(B HEPCGN_10840 0.81 -1.3 910,504 - tetR(B HEPCGN_10840 0.81 +0.4 910,520 - tetR(B HEPCGN_10840 0.84 -0.3 910,520 - tetR(B HEPCGN_10840 0.84 -0.5 910,520 - tetR(B HEPCGN_10840 0.84 -0.7 910,609 + +1.1 910,735 - -0.5 910,934 + tet(B HEPCGN_10845 0.19 +0.6 910,959 - tet(B HEPCGN_10845 0.21 +0.2
Or see this region's nucleotide sequence