Experiment: Bas05
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hflX and hfq are separated by 76 nucleotides hfq and miaA are separated by 85 nucleotides miaA and mutL overlap by 8 nucleotides
HEPCGN_10480: hflX - GTPase HflX, at 838,108 to 839,388
hflX
HEPCGN_10485: hfq - RNA chaperone Hfq, at 839,465 to 839,773
hfq
HEPCGN_10490: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 839,859 to 840,809
miaA
HEPCGN_10495: mutL - DNA mismatch repair endonuclease MutL, at 840,802 to 842,649
mutL
Position (kb) 839
840
841 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 838.868 kb on - strand, within hflX at 839.028 kb on + strand, within hflX at 839.029 kb on - strand, within hflX at 839.223 kb on - strand, within hflX at 839.242 kb on - strand, within hflX at 839.347 kb on + strand at 839.348 kb on - strand at 839.348 kb on - strand at 839.393 kb on - strand at 839.393 kb on - strand at 839.394 kb on + strand at 839.395 kb on - strand at 839.406 kb on + strand at 839.526 kb on + strand, within hfq at 839.531 kb on - strand, within hfq at 839.531 kb on - strand, within hfq at 839.558 kb on - strand, within hfq at 839.790 kb on - strand at 839.790 kb on - strand at 839.790 kb on - strand at 839.790 kb on - strand at 839.790 kb on - strand at 839.840 kb on - strand at 840.041 kb on - strand, within miaA at 840.041 kb on - strand, within miaA at 840.232 kb on - strand, within miaA at 840.478 kb on + strand, within miaA at 840.574 kb on + strand, within miaA at 840.674 kb on + strand, within miaA at 840.702 kb on + strand, within miaA at 840.703 kb on - strand, within miaA at 840.800 kb on + strand at 840.824 kb on - strand at 840.835 kb on + strand at 840.850 kb on + strand at 840.889 kb on + strand at 840.889 kb on + strand at 841.016 kb on + strand, within mutL at 841.016 kb on + strand, within mutL at 841.016 kb on + strand, within mutL at 841.017 kb on - strand, within mutL at 841.021 kb on + strand, within mutL at 841.061 kb on - strand, within mutL at 841.329 kb on + strand, within mutL at 841.437 kb on - strand, within mutL at 841.524 kb on - strand, within mutL at 841.534 kb on + strand, within mutL at 841.682 kb on + strand, within mutL at 841.779 kb on - strand, within mutL
Per-strain Table
Position Strand Gene LocusTag Fraction Bas05 remove 838,868 - hflX HEPCGN_10480 0.59 +0.1 839,028 + hflX HEPCGN_10480 0.72 -0.5 839,029 - hflX HEPCGN_10480 0.72 -0.5 839,223 - hflX HEPCGN_10480 0.87 +0.0 839,242 - hflX HEPCGN_10480 0.89 +1.1 839,347 + +0.1 839,348 - +1.0 839,348 - +0.1 839,393 - -0.8 839,393 - -0.6 839,394 + +0.0 839,395 - -0.4 839,406 + +0.1 839,526 + hfq HEPCGN_10485 0.20 +0.6 839,531 - hfq HEPCGN_10485 0.21 -0.2 839,531 - hfq HEPCGN_10485 0.21 -1.6 839,558 - hfq HEPCGN_10485 0.30 -0.4 839,790 - +1.1 839,790 - -0.5 839,790 - -0.2 839,790 - -0.3 839,790 - +1.4 839,840 - +0.1 840,041 - miaA HEPCGN_10490 0.19 -1.5 840,041 - miaA HEPCGN_10490 0.19 -0.6 840,232 - miaA HEPCGN_10490 0.39 -0.5 840,478 + miaA HEPCGN_10490 0.65 -0.9 840,574 + miaA HEPCGN_10490 0.75 -0.9 840,674 + miaA HEPCGN_10490 0.86 +0.2 840,702 + miaA HEPCGN_10490 0.89 -2.1 840,703 - miaA HEPCGN_10490 0.89 -1.3 840,800 + -0.7 840,824 - +0.4 840,835 + +0.0 840,850 + -0.5 840,889 + +0.4 840,889 + -1.1 841,016 + mutL HEPCGN_10495 0.12 -0.6 841,016 + mutL HEPCGN_10495 0.12 -0.5 841,016 + mutL HEPCGN_10495 0.12 +0.2 841,017 - mutL HEPCGN_10495 0.12 -0.2 841,021 + mutL HEPCGN_10495 0.12 -0.3 841,061 - mutL HEPCGN_10495 0.14 +3.2 841,329 + mutL HEPCGN_10495 0.29 +0.0 841,437 - mutL HEPCGN_10495 0.34 -0.4 841,524 - mutL HEPCGN_10495 0.39 -0.4 841,534 + mutL HEPCGN_10495 0.40 -0.0 841,682 + mutL HEPCGN_10495 0.48 -0.0 841,779 - mutL HEPCGN_10495 0.53 -0.6
Or see this region's nucleotide sequence