Experiment: JK32
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmKL and ycbX are separated by 243 nucleotides ycbX and zapC overlap by 4 nucleotides zapC and pyrD are separated by 173 nucleotides
BNILDI_20000: rlmKL - bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL, at 4,014,259 to 4,016,367
rlmKL
BNILDI_20005: ycbX - 6-N-hydroxylaminopurine resistance protein YcbX, at 4,016,611 to 4,017,720
ycbX
BNILDI_20010: zapC - cell division protein ZapC, at 4,017,717 to 4,018,259
zapC
BNILDI_20015: pyrD - quinone-dependent dihydroorotate dehydrogenase, at 4,018,433 to 4,019,443
pyrD
Position (kb)
4016
4017
4018 Strain fitness (log2 ratio)
-1
0
1
2 at 4015.665 kb on + strand, within rlmKL at 4015.665 kb on + strand, within rlmKL at 4015.891 kb on + strand, within rlmKL at 4015.936 kb on - strand, within rlmKL at 4016.151 kb on - strand, within rlmKL at 4016.176 kb on - strand at 4016.196 kb on + strand at 4016.196 kb on + strand at 4016.197 kb on - strand at 4016.197 kb on - strand at 4016.384 kb on + strand at 4016.406 kb on + strand at 4016.406 kb on + strand at 4016.406 kb on + strand at 4016.464 kb on - strand at 4016.464 kb on - strand at 4016.510 kb on + strand at 4016.551 kb on + strand at 4016.551 kb on + strand at 4016.556 kb on - strand at 4016.558 kb on + strand at 4016.559 kb on - strand at 4016.588 kb on + strand at 4016.622 kb on - strand at 4016.670 kb on + strand at 4016.671 kb on - strand at 4016.739 kb on + strand, within ycbX at 4016.745 kb on + strand, within ycbX at 4016.746 kb on - strand, within ycbX at 4016.840 kb on - strand, within ycbX at 4016.840 kb on - strand, within ycbX at 4016.939 kb on + strand, within ycbX at 4016.970 kb on - strand, within ycbX at 4016.970 kb on - strand, within ycbX at 4017.108 kb on - strand, within ycbX at 4017.168 kb on + strand, within ycbX at 4017.482 kb on + strand, within ycbX at 4017.486 kb on + strand, within ycbX at 4017.486 kb on + strand, within ycbX at 4017.486 kb on + strand, within ycbX at 4017.520 kb on - strand, within ycbX at 4017.617 kb on + strand at 4017.681 kb on + strand at 4017.705 kb on + strand at 4017.705 kb on + strand at 4017.706 kb on - strand at 4017.706 kb on - strand at 4017.753 kb on - strand at 4017.843 kb on + strand, within zapC at 4017.850 kb on - strand, within zapC at 4017.850 kb on - strand, within zapC at 4018.050 kb on - strand, within zapC at 4018.053 kb on + strand, within zapC at 4018.174 kb on - strand, within zapC at 4018.179 kb on - strand, within zapC at 4018.179 kb on - strand, within zapC at 4018.179 kb on - strand, within zapC at 4018.226 kb on - strand at 4018.278 kb on - strand at 4018.279 kb on + strand at 4018.368 kb on + strand at 4018.457 kb on - strand at 4018.610 kb on - strand, within pyrD
Per-strain Table
Position Strand Gene LocusTag Fraction JK32 remove 4,015,665 + rlmKL BNILDI_20000 0.67 -0.9 4,015,665 + rlmKL BNILDI_20000 0.67 +0.2 4,015,891 + rlmKL BNILDI_20000 0.77 +0.0 4,015,936 - rlmKL BNILDI_20000 0.80 +1.2 4,016,151 - rlmKL BNILDI_20000 0.90 -0.3 4,016,176 - +0.2 4,016,196 + -0.2 4,016,196 + +2.3 4,016,197 - +0.2 4,016,197 - -1.6 4,016,384 + +1.0 4,016,406 + +2.5 4,016,406 + +0.4 4,016,406 + +0.3 4,016,464 - +1.0 4,016,464 - +0.0 4,016,510 + -0.5 4,016,551 + -0.7 4,016,551 + +0.5 4,016,556 - -1.7 4,016,558 + +0.1 4,016,559 - -0.3 4,016,588 + +0.1 4,016,622 - +0.1 4,016,670 + -0.4 4,016,671 - +0.4 4,016,739 + ycbX BNILDI_20005 0.12 -0.3 4,016,745 + ycbX BNILDI_20005 0.12 +0.1 4,016,746 - ycbX BNILDI_20005 0.12 -0.4 4,016,840 - ycbX BNILDI_20005 0.21 +0.3 4,016,840 - ycbX BNILDI_20005 0.21 -0.6 4,016,939 + ycbX BNILDI_20005 0.30 +0.1 4,016,970 - ycbX BNILDI_20005 0.32 +1.1 4,016,970 - ycbX BNILDI_20005 0.32 +0.1 4,017,108 - ycbX BNILDI_20005 0.45 +0.3 4,017,168 + ycbX BNILDI_20005 0.50 +0.0 4,017,482 + ycbX BNILDI_20005 0.78 +0.2 4,017,486 + ycbX BNILDI_20005 0.79 -0.2 4,017,486 + ycbX BNILDI_20005 0.79 +0.3 4,017,486 + ycbX BNILDI_20005 0.79 +0.5 4,017,520 - ycbX BNILDI_20005 0.82 +0.9 4,017,617 + +0.5 4,017,681 + -0.3 4,017,705 + -0.2 4,017,705 + -1.2 4,017,706 - -0.2 4,017,706 - +0.1 4,017,753 - -1.2 4,017,843 + zapC BNILDI_20010 0.23 +0.5 4,017,850 - zapC BNILDI_20010 0.24 -0.3 4,017,850 - zapC BNILDI_20010 0.24 +1.1 4,018,050 - zapC BNILDI_20010 0.61 +0.4 4,018,053 + zapC BNILDI_20010 0.62 +1.6 4,018,174 - zapC BNILDI_20010 0.84 -0.8 4,018,179 - zapC BNILDI_20010 0.85 -1.2 4,018,179 - zapC BNILDI_20010 0.85 -0.9 4,018,179 - zapC BNILDI_20010 0.85 +0.0 4,018,226 - -0.4 4,018,278 - -1.2 4,018,279 + -0.1 4,018,368 + +0.6 4,018,457 - +0.3 4,018,610 - pyrD BNILDI_20015 0.18 -0.5
Or see this region's nucleotide sequence