Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00250

Experiment: Phage M13-2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00249 and OHPLBJKB_00250 are separated by 396 nucleotidesOHPLBJKB_00250 and OHPLBJKB_00251 are separated by 97 nucleotidesOHPLBJKB_00251 and OHPLBJKB_00252 overlap by 4 nucleotides OHPLBJKB_00249: OHPLBJKB_00249 - High-affinity branched-chain amino acid transport ATP-binding protein LivF, at 271,208 to 271,921 _00249 OHPLBJKB_00250: OHPLBJKB_00250 - sn-glycerol-3-phosphate-binding periplasmic protein UgpB, at 272,318 to 273,634 _00250 OHPLBJKB_00251: OHPLBJKB_00251 - sn-glycerol-3-phosphate transport system permease protein UgpA, at 273,732 to 274,619 _00251 OHPLBJKB_00252: OHPLBJKB_00252 - Lactose transport system permease protein LacG, at 274,616 to 275,461 _00252 Position (kb) 272 273 274Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6at 271.355 kb on - strand, within OHPLBJKB_00249at 271.557 kb on + strand, within OHPLBJKB_00249at 271.568 kb on + strand, within OHPLBJKB_00249at 271.568 kb on + strand, within OHPLBJKB_00249at 271.569 kb on - strand, within OHPLBJKB_00249at 271.569 kb on - strand, within OHPLBJKB_00249at 271.687 kb on - strand, within OHPLBJKB_00249at 271.774 kb on + strand, within OHPLBJKB_00249at 271.774 kb on + strand, within OHPLBJKB_00249at 271.774 kb on + strand, within OHPLBJKB_00249at 271.775 kb on - strand, within OHPLBJKB_00249at 271.775 kb on - strand, within OHPLBJKB_00249at 271.809 kb on + strand, within OHPLBJKB_00249at 271.810 kb on - strand, within OHPLBJKB_00249at 271.829 kb on + strand, within OHPLBJKB_00249at 271.923 kb on - strandat 272.009 kb on + strandat 272.027 kb on - strandat 272.050 kb on - strandat 272.095 kb on + strandat 272.102 kb on + strandat 272.202 kb on + strandat 272.202 kb on + strandat 272.202 kb on + strandat 272.202 kb on + strandat 272.203 kb on - strandat 272.203 kb on - strandat 272.205 kb on + strandat 272.205 kb on + strandat 272.205 kb on + strandat 272.205 kb on + strandat 272.205 kb on + strandat 272.206 kb on - strandat 272.206 kb on - strandat 272.206 kb on - strandat 272.298 kb on + strandat 272.312 kb on + strandat 272.312 kb on + strandat 272.313 kb on - strandat 272.328 kb on + strandat 272.333 kb on + strandat 272.334 kb on - strandat 272.334 kb on - strandat 272.334 kb on - strandat 272.335 kb on + strandat 272.335 kb on + strandat 272.336 kb on - strandat 272.336 kb on - strandat 272.413 kb on + strandat 272.414 kb on - strandat 272.414 kb on - strandat 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.498 kb on + strand, within OHPLBJKB_00250at 272.499 kb on - strand, within OHPLBJKB_00250at 272.499 kb on - strand, within OHPLBJKB_00250at 272.499 kb on - strand, within OHPLBJKB_00250at 272.501 kb on - strand, within OHPLBJKB_00250at 272.510 kb on + strand, within OHPLBJKB_00250at 272.510 kb on + strand, within OHPLBJKB_00250at 272.510 kb on + strand, within OHPLBJKB_00250at 272.510 kb on + strand, within OHPLBJKB_00250at 272.510 kb on + strand, within OHPLBJKB_00250at 272.511 kb on - strand, within OHPLBJKB_00250at 272.511 kb on - strand, within OHPLBJKB_00250at 272.511 kb on - strand, within OHPLBJKB_00250at 272.523 kb on + strand, within OHPLBJKB_00250at 272.580 kb on - strand, within OHPLBJKB_00250at 272.621 kb on - strand, within OHPLBJKB_00250at 272.630 kb on + strand, within OHPLBJKB_00250at 272.630 kb on + strand, within OHPLBJKB_00250at 272.630 kb on + strand, within OHPLBJKB_00250at 272.630 kb on + strand, within OHPLBJKB_00250at 272.631 kb on - strand, within OHPLBJKB_00250at 272.631 kb on - strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.724 kb on + strand, within OHPLBJKB_00250at 272.725 kb on - strand, within OHPLBJKB_00250at 272.725 kb on - strand, within OHPLBJKB_00250at 272.763 kb on - strand, within OHPLBJKB_00250at 272.763 kb on - strand, within OHPLBJKB_00250at 272.765 kb on + strand, within OHPLBJKB_00250at 272.766 kb on - strand, within OHPLBJKB_00250at 272.766 kb on - strand, within OHPLBJKB_00250at 272.796 kb on + strand, within OHPLBJKB_00250at 272.797 kb on - strand, within OHPLBJKB_00250at 272.797 kb on - strand, within OHPLBJKB_00250at 272.840 kb on + strand, within OHPLBJKB_00250at 272.840 kb on + strand, within OHPLBJKB_00250at 272.841 kb on - strand, within OHPLBJKB_00250at 272.841 kb on - strand, within OHPLBJKB_00250at 273.060 kb on - strand, within OHPLBJKB_00250at 273.095 kb on + strand, within OHPLBJKB_00250at 273.096 kb on - strand, within OHPLBJKB_00250at 273.191 kb on + strand, within OHPLBJKB_00250at 273.278 kb on + strand, within OHPLBJKB_00250at 273.279 kb on - strand, within OHPLBJKB_00250at 273.279 kb on - strand, within OHPLBJKB_00250at 273.279 kb on - strand, within OHPLBJKB_00250at 273.281 kb on - strand, within OHPLBJKB_00250at 273.377 kb on + strand, within OHPLBJKB_00250at 273.377 kb on + strand, within OHPLBJKB_00250at 273.378 kb on - strand, within OHPLBJKB_00250at 273.404 kb on + strand, within OHPLBJKB_00250at 273.405 kb on - strand, within OHPLBJKB_00250at 273.641 kb on + strandat 273.642 kb on - strandat 273.642 kb on - strandat 273.662 kb on + strandat 273.731 kb on - strandat 273.774 kb on + strandat 273.775 kb on - strandat 273.775 kb on - strandat 273.846 kb on + strand, within OHPLBJKB_00251at 273.853 kb on + strand, within OHPLBJKB_00251at 273.854 kb on - strand, within OHPLBJKB_00251at 273.903 kb on - strand, within OHPLBJKB_00251at 273.930 kb on + strand, within OHPLBJKB_00251at 273.930 kb on + strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.931 kb on - strand, within OHPLBJKB_00251at 273.933 kb on + strand, within OHPLBJKB_00251at 273.933 kb on + strand, within OHPLBJKB_00251at 273.933 kb on + strand, within OHPLBJKB_00251at 273.933 kb on + strand, within OHPLBJKB_00251at 273.933 kb on + strand, within OHPLBJKB_00251at 273.934 kb on - strand, within OHPLBJKB_00251at 273.958 kb on + strand, within OHPLBJKB_00251at 274.026 kb on + strand, within OHPLBJKB_00251at 274.026 kb on + strand, within OHPLBJKB_00251at 274.026 kb on + strand, within OHPLBJKB_00251at 274.026 kb on + strand, within OHPLBJKB_00251at 274.027 kb on - strand, within OHPLBJKB_00251at 274.027 kb on - strand, within OHPLBJKB_00251at 274.027 kb on - strand, within OHPLBJKB_00251at 274.075 kb on - strand, within OHPLBJKB_00251at 274.228 kb on + strand, within OHPLBJKB_00251at 274.263 kb on + strand, within OHPLBJKB_00251at 274.263 kb on + strand, within OHPLBJKB_00251at 274.264 kb on - strand, within OHPLBJKB_00251at 274.264 kb on - strand, within OHPLBJKB_00251at 274.343 kb on + strand, within OHPLBJKB_00251at 274.404 kb on + strand, within OHPLBJKB_00251at 274.404 kb on + strand, within OHPLBJKB_00251at 274.404 kb on + strand, within OHPLBJKB_00251at 274.404 kb on + strand, within OHPLBJKB_00251at 274.405 kb on - strand, within OHPLBJKB_00251at 274.482 kb on + strand, within OHPLBJKB_00251at 274.482 kb on + strand, within OHPLBJKB_00251at 274.483 kb on - strand, within OHPLBJKB_00251at 274.483 kb on - strand, within OHPLBJKB_00251at 274.484 kb on + strand, within OHPLBJKB_00251at 274.484 kb on + strand, within OHPLBJKB_00251at 274.484 kb on + strand, within OHPLBJKB_00251at 274.590 kb on + strandat 274.591 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Phage M13-2
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271,355 - OHPLBJKB_00249 0.21 -1.1
271,557 + OHPLBJKB_00249 0.49 +0.1
271,568 + OHPLBJKB_00249 0.50 +0.2
271,568 + OHPLBJKB_00249 0.50 +0.3
271,569 - OHPLBJKB_00249 0.51 -0.2
271,569 - OHPLBJKB_00249 0.51 -0.7
271,687 - OHPLBJKB_00249 0.67 +1.2
271,774 + OHPLBJKB_00249 0.79 +0.0
271,774 + OHPLBJKB_00249 0.79 -0.1
271,774 + OHPLBJKB_00249 0.79 +1.9
271,775 - OHPLBJKB_00249 0.79 +0.0
271,775 - OHPLBJKB_00249 0.79 +0.2
271,809 + OHPLBJKB_00249 0.84 -1.0
271,810 - OHPLBJKB_00249 0.84 -0.7
271,829 + OHPLBJKB_00249 0.87 +0.3
271,923 - -0.9
272,009 + -0.8
272,027 - -0.8
272,050 - -2.2
272,095 + -0.6
272,102 + -0.3
272,202 + +0.9
272,202 + +0.7
272,202 + -0.1
272,202 + +0.9
272,203 - -1.5
272,203 - +0.2
272,205 + -0.1
272,205 + +0.2
272,205 + -1.3
272,205 + -2.3
272,205 + +0.5
272,206 - -0.5
272,206 - -0.3
272,206 - -0.9
272,298 + +0.0
272,312 + -0.7
272,312 + -0.1
272,313 - +0.6
272,328 + +0.7
272,333 + -0.2
272,334 - -0.0
272,334 - +0.4
272,334 - +1.2
272,335 + -0.4
272,335 + +1.2
272,336 - +0.1
272,336 - +0.7
272,413 + +0.3
272,414 - -0.1
272,414 - +0.3
272,498 + OHPLBJKB_00250 0.14 +0.8
272,498 + OHPLBJKB_00250 0.14 +0.2
272,498 + OHPLBJKB_00250 0.14 -0.1
272,498 + OHPLBJKB_00250 0.14 +1.5
272,498 + OHPLBJKB_00250 0.14 -0.3
272,498 + OHPLBJKB_00250 0.14 -0.5
272,498 + OHPLBJKB_00250 0.14 -0.1
272,498 + OHPLBJKB_00250 0.14 -1.1
272,499 - OHPLBJKB_00250 0.14 +0.4
272,499 - OHPLBJKB_00250 0.14 +0.2
272,499 - OHPLBJKB_00250 0.14 -0.8
272,501 - OHPLBJKB_00250 0.14 -1.4
272,510 + OHPLBJKB_00250 0.15 -0.0
272,510 + OHPLBJKB_00250 0.15 +0.7
272,510 + OHPLBJKB_00250 0.15 -1.0
272,510 + OHPLBJKB_00250 0.15 -0.0
272,510 + OHPLBJKB_00250 0.15 -2.4
272,511 - OHPLBJKB_00250 0.15 +0.9
272,511 - OHPLBJKB_00250 0.15 -0.3
272,511 - OHPLBJKB_00250 0.15 +0.2
272,523 + OHPLBJKB_00250 0.16 -0.3
272,580 - OHPLBJKB_00250 0.20 +0.4
272,621 - OHPLBJKB_00250 0.23 +0.0
272,630 + OHPLBJKB_00250 0.24 -0.0
272,630 + OHPLBJKB_00250 0.24 -0.2
272,630 + OHPLBJKB_00250 0.24 -0.4
272,630 + OHPLBJKB_00250 0.24 -0.2
272,631 - OHPLBJKB_00250 0.24 +0.8
272,631 - OHPLBJKB_00250 0.24 -1.5
272,724 + OHPLBJKB_00250 0.31 -2.0
272,724 + OHPLBJKB_00250 0.31 +0.0
272,724 + OHPLBJKB_00250 0.31 -2.3
272,724 + OHPLBJKB_00250 0.31 -0.1
272,724 + OHPLBJKB_00250 0.31 -0.7
272,724 + OHPLBJKB_00250 0.31 -0.2
272,724 + OHPLBJKB_00250 0.31 +0.5
272,725 - OHPLBJKB_00250 0.31 +0.1
272,725 - OHPLBJKB_00250 0.31 +0.2
272,763 - OHPLBJKB_00250 0.34 +0.7
272,763 - OHPLBJKB_00250 0.34 -0.5
272,765 + OHPLBJKB_00250 0.34 +0.1
272,766 - OHPLBJKB_00250 0.34 -2.5
272,766 - OHPLBJKB_00250 0.34 -0.0
272,796 + OHPLBJKB_00250 0.36 -1.2
272,797 - OHPLBJKB_00250 0.36 +0.7
272,797 - OHPLBJKB_00250 0.36 -0.5
272,840 + OHPLBJKB_00250 0.40 -0.8
272,840 + OHPLBJKB_00250 0.40 -0.0
272,841 - OHPLBJKB_00250 0.40 +0.1
272,841 - OHPLBJKB_00250 0.40 +0.9
273,060 - OHPLBJKB_00250 0.56 -2.5
273,095 + OHPLBJKB_00250 0.59 +0.8
273,096 - OHPLBJKB_00250 0.59 +0.5
273,191 + OHPLBJKB_00250 0.66 +0.0
273,278 + OHPLBJKB_00250 0.73 +0.4
273,279 - OHPLBJKB_00250 0.73 -1.4
273,279 - OHPLBJKB_00250 0.73 +0.8
273,279 - OHPLBJKB_00250 0.73 +0.3
273,281 - OHPLBJKB_00250 0.73 -0.1
273,377 + OHPLBJKB_00250 0.80 -1.5
273,377 + OHPLBJKB_00250 0.80 +0.1
273,378 - OHPLBJKB_00250 0.80 +0.1
273,404 + OHPLBJKB_00250 0.82 -2.1
273,405 - OHPLBJKB_00250 0.83 -0.3
273,641 + -0.3
273,642 - -0.8
273,642 - -0.5
273,662 + +1.0
273,731 - -1.8
273,774 + -1.0
273,775 - +0.2
273,775 - +2.1
273,846 + OHPLBJKB_00251 0.13 +1.0
273,853 + OHPLBJKB_00251 0.14 -0.8
273,854 - OHPLBJKB_00251 0.14 -0.9
273,903 - OHPLBJKB_00251 0.19 +0.4
273,930 + OHPLBJKB_00251 0.22 +0.2
273,930 + OHPLBJKB_00251 0.22 +1.4
273,931 - OHPLBJKB_00251 0.22 -0.7
273,931 - OHPLBJKB_00251 0.22 -0.6
273,931 - OHPLBJKB_00251 0.22 +1.0
273,931 - OHPLBJKB_00251 0.22 -0.8
273,931 - OHPLBJKB_00251 0.22 +0.4
273,931 - OHPLBJKB_00251 0.22 +0.0
273,931 - OHPLBJKB_00251 0.22 -0.5
273,931 - OHPLBJKB_00251 0.22 -1.1
273,931 - OHPLBJKB_00251 0.22 -0.5
273,933 + OHPLBJKB_00251 0.23 +0.6
273,933 + OHPLBJKB_00251 0.23 -0.0
273,933 + OHPLBJKB_00251 0.23 -0.6
273,933 + OHPLBJKB_00251 0.23 -0.4
273,933 + OHPLBJKB_00251 0.23 -0.9
273,934 - OHPLBJKB_00251 0.23 -1.3
273,958 + OHPLBJKB_00251 0.25 -0.7
274,026 + OHPLBJKB_00251 0.33 -0.1
274,026 + OHPLBJKB_00251 0.33 +0.1
274,026 + OHPLBJKB_00251 0.33 +6.5
274,026 + OHPLBJKB_00251 0.33 -2.0
274,027 - OHPLBJKB_00251 0.33 -0.9
274,027 - OHPLBJKB_00251 0.33 +0.7
274,027 - OHPLBJKB_00251 0.33 -0.6
274,075 - OHPLBJKB_00251 0.39 -0.1
274,228 + OHPLBJKB_00251 0.56 +0.4
274,263 + OHPLBJKB_00251 0.60 +1.2
274,263 + OHPLBJKB_00251 0.60 -1.3
274,264 - OHPLBJKB_00251 0.60 -0.0
274,264 - OHPLBJKB_00251 0.60 +0.9
274,343 + OHPLBJKB_00251 0.69 +0.6
274,404 + OHPLBJKB_00251 0.76 -0.3
274,404 + OHPLBJKB_00251 0.76 -0.7
274,404 + OHPLBJKB_00251 0.76 -0.1
274,404 + OHPLBJKB_00251 0.76 -1.9
274,405 - OHPLBJKB_00251 0.76 -0.3
274,482 + OHPLBJKB_00251 0.84 +1.6
274,482 + OHPLBJKB_00251 0.84 +0.7
274,483 - OHPLBJKB_00251 0.85 -2.0
274,483 - OHPLBJKB_00251 0.85 -0.5
274,484 + OHPLBJKB_00251 0.85 -1.7
274,484 + OHPLBJKB_00251 0.85 -0.2
274,484 + OHPLBJKB_00251 0.85 -0.3
274,590 + -1.0
274,591 - -2.7

Or see this region's nucleotide sequence