Experiment: Control_ECRC62
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaC and wcaD overlap by 26 nucleotides wcaD and wcaE are separated by 10 nucleotides wcaE and wcaF are separated by 15 nucleotides
BNILDI_14565: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 2,942,499 to 2,943,716
wcaC
BNILDI_14570: wcaD - putative colanic acid polymerase WcaD, at 2,943,691 to 2,944,908
wcaD
BNILDI_14575: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 2,944,919 to 2,945,665
wcaE
BNILDI_14580: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 2,945,681 to 2,946,229
wcaF
Position (kb)
2943
2944
2945 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2942.752 kb on + strand, within wcaC at 2942.829 kb on - strand, within wcaC at 2942.984 kb on - strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.987 kb on + strand, within wcaC at 2942.988 kb on - strand, within wcaC at 2943.046 kb on + strand, within wcaC at 2943.152 kb on + strand, within wcaC at 2943.152 kb on + strand, within wcaC at 2943.323 kb on + strand, within wcaC at 2943.454 kb on - strand, within wcaC at 2943.616 kb on + strand at 2943.690 kb on + strand at 2943.691 kb on - strand at 2943.714 kb on + strand at 2943.718 kb on + strand at 2943.719 kb on - strand at 2943.719 kb on - strand at 2943.719 kb on - strand at 2943.739 kb on + strand at 2943.830 kb on + strand, within wcaD at 2943.830 kb on + strand, within wcaD at 2943.831 kb on - strand, within wcaD at 2943.898 kb on + strand, within wcaD at 2943.931 kb on + strand, within wcaD at 2943.959 kb on - strand, within wcaD at 2943.961 kb on - strand, within wcaD at 2943.973 kb on - strand, within wcaD at 2943.991 kb on - strand, within wcaD at 2944.035 kb on - strand, within wcaD at 2944.965 kb on + strand at 2944.966 kb on - strand at 2945.011 kb on + strand, within wcaE at 2945.012 kb on - strand, within wcaE at 2945.653 kb on + strand at 2945.878 kb on + strand, within wcaF
Per-strain Table
Position Strand Gene LocusTag Fraction Control_ECRC62 remove 2,942,752 + wcaC BNILDI_14565 0.21 +0.9 2,942,829 - wcaC BNILDI_14565 0.27 -0.0 2,942,984 - wcaC BNILDI_14565 0.40 +0.1 2,942,987 + wcaC BNILDI_14565 0.40 +1.3 2,942,987 + wcaC BNILDI_14565 0.40 +1.7 2,942,987 + wcaC BNILDI_14565 0.40 -0.3 2,942,987 + wcaC BNILDI_14565 0.40 +1.3 2,942,988 - wcaC BNILDI_14565 0.40 -0.9 2,943,046 + wcaC BNILDI_14565 0.45 -2.3 2,943,152 + wcaC BNILDI_14565 0.54 -1.2 2,943,152 + wcaC BNILDI_14565 0.54 -0.4 2,943,323 + wcaC BNILDI_14565 0.68 +2.6 2,943,454 - wcaC BNILDI_14565 0.78 -0.7 2,943,616 + +0.1 2,943,690 + +0.4 2,943,691 - +1.0 2,943,714 + +0.4 2,943,718 + +0.8 2,943,719 - +2.1 2,943,719 - -0.9 2,943,719 - +0.3 2,943,739 + -1.6 2,943,830 + wcaD BNILDI_14570 0.11 -1.3 2,943,830 + wcaD BNILDI_14570 0.11 +0.1 2,943,831 - wcaD BNILDI_14570 0.11 +0.4 2,943,898 + wcaD BNILDI_14570 0.17 -0.6 2,943,931 + wcaD BNILDI_14570 0.20 +0.6 2,943,959 - wcaD BNILDI_14570 0.22 +1.4 2,943,961 - wcaD BNILDI_14570 0.22 -1.5 2,943,973 - wcaD BNILDI_14570 0.23 -1.3 2,943,991 - wcaD BNILDI_14570 0.25 -1.9 2,944,035 - wcaD BNILDI_14570 0.28 -0.3 2,944,965 + -1.9 2,944,966 - -1.5 2,945,011 + wcaE BNILDI_14575 0.12 +1.4 2,945,012 - wcaE BNILDI_14575 0.12 +1.7 2,945,653 + +0.5 2,945,878 + wcaF BNILDI_14580 0.36 -0.7
Or see this region's nucleotide sequence