Strain Fitness in Escherichia coli ECRC62 around BNILDI_14070

Experiment: Control_ECRC62

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcdd and yohK are separated by 129 nucleotidesyohK and yohJ overlap by 4 nucleotidesyohJ and lysR are separated by 130 nucleotides BNILDI_14060: cdd - cytidine deaminase, at 2,834,435 to 2,835,319 cdd BNILDI_14065: yohK - CidB/LrgB family autolysis modulator, at 2,835,449 to 2,836,144 yohK BNILDI_14070: yohJ - UPF0299 membrane protein YohJ, at 2,836,141 to 2,836,539 yohJ BNILDI_14075: lysR - transcriptional regulator, at 2,836,670 to 2,837,578 lysR Position (kb) 2836 2837Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 2835.152 kb on + strand, within cddat 2835.238 kb on - strandat 2835.254 kb on - strandat 2835.333 kb on - strandat 2835.341 kb on - strandat 2835.356 kb on + strandat 2835.356 kb on + strandat 2835.357 kb on - strandat 2835.375 kb on + strandat 2835.375 kb on + strandat 2835.377 kb on + strandat 2835.401 kb on - strandat 2835.459 kb on - strandat 2835.502 kb on + strandat 2835.502 kb on + strandat 2835.507 kb on - strandat 2835.536 kb on - strand, within yohKat 2835.656 kb on - strand, within yohKat 2835.666 kb on - strand, within yohKat 2835.912 kb on + strand, within yohKat 2835.914 kb on + strand, within yohKat 2835.922 kb on - strand, within yohKat 2835.982 kb on - strand, within yohKat 2835.982 kb on - strand, within yohKat 2836.011 kb on - strand, within yohKat 2836.066 kb on + strand, within yohKat 2836.067 kb on - strand, within yohKat 2836.067 kb on - strand, within yohKat 2836.165 kb on + strandat 2836.173 kb on + strandat 2836.186 kb on + strand, within yohJat 2836.231 kb on - strand, within yohJat 2836.329 kb on + strand, within yohJat 2836.331 kb on + strand, within yohJat 2836.332 kb on - strand, within yohJat 2836.343 kb on - strand, within yohJat 2836.346 kb on + strand, within yohJat 2836.346 kb on + strand, within yohJat 2836.347 kb on - strand, within yohJat 2836.347 kb on - strand, within yohJat 2836.484 kb on + strand, within yohJat 2836.621 kb on + strandat 2836.641 kb on - strandat 2836.641 kb on - strandat 2836.671 kb on + strandat 2836.672 kb on - strandat 2836.672 kb on - strandat 2836.704 kb on - strandat 2836.843 kb on + strand, within lysRat 2836.844 kb on - strand, within lysRat 2837.020 kb on - strand, within lysRat 2837.020 kb on - strand, within lysRat 2837.020 kb on - strand, within lysRat 2837.070 kb on + strand, within lysRat 2837.082 kb on + strand, within lysRat 2837.198 kb on + strand, within lysRat 2837.198 kb on + strand, within lysRat 2837.480 kb on - strand, within lysR

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Per-strain Table

Position Strand Gene LocusTag Fraction Control_ECRC62
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2,835,152 + cdd BNILDI_14060 0.81 -3.0
2,835,238 - +3.2
2,835,254 - +0.7
2,835,333 - +0.1
2,835,341 - -0.4
2,835,356 + +0.3
2,835,356 + -0.8
2,835,357 - +0.3
2,835,375 + +5.3
2,835,375 + -0.2
2,835,377 + +0.6
2,835,401 - -1.2
2,835,459 - -0.3
2,835,502 + +0.8
2,835,502 + -0.8
2,835,507 - -0.6
2,835,536 - yohK BNILDI_14065 0.12 -0.1
2,835,656 - yohK BNILDI_14065 0.30 +0.7
2,835,666 - yohK BNILDI_14065 0.31 +0.8
2,835,912 + yohK BNILDI_14065 0.67 -0.3
2,835,914 + yohK BNILDI_14065 0.67 -0.8
2,835,922 - yohK BNILDI_14065 0.68 +0.4
2,835,982 - yohK BNILDI_14065 0.77 -0.8
2,835,982 - yohK BNILDI_14065 0.77 -1.7
2,836,011 - yohK BNILDI_14065 0.81 +0.1
2,836,066 + yohK BNILDI_14065 0.89 -0.7
2,836,067 - yohK BNILDI_14065 0.89 +2.0
2,836,067 - yohK BNILDI_14065 0.89 -0.2
2,836,165 + +3.7
2,836,173 + +0.7
2,836,186 + yohJ BNILDI_14070 0.11 +1.8
2,836,231 - yohJ BNILDI_14070 0.23 -0.4
2,836,329 + yohJ BNILDI_14070 0.47 -0.4
2,836,331 + yohJ BNILDI_14070 0.48 +0.7
2,836,332 - yohJ BNILDI_14070 0.48 -0.2
2,836,343 - yohJ BNILDI_14070 0.51 +1.1
2,836,346 + yohJ BNILDI_14070 0.51 +0.4
2,836,346 + yohJ BNILDI_14070 0.51 -1.3
2,836,347 - yohJ BNILDI_14070 0.52 -0.0
2,836,347 - yohJ BNILDI_14070 0.52 -0.6
2,836,484 + yohJ BNILDI_14070 0.86 -0.0
2,836,621 + -0.1
2,836,641 - -0.6
2,836,641 - -0.1
2,836,671 + +0.9
2,836,672 - +1.2
2,836,672 - +1.0
2,836,704 - -0.3
2,836,843 + lysR BNILDI_14075 0.19 +0.4
2,836,844 - lysR BNILDI_14075 0.19 -0.2
2,837,020 - lysR BNILDI_14075 0.39 -0.7
2,837,020 - lysR BNILDI_14075 0.39 -2.1
2,837,020 - lysR BNILDI_14075 0.39 -2.3
2,837,070 + lysR BNILDI_14075 0.44 -0.2
2,837,082 + lysR BNILDI_14075 0.45 -1.5
2,837,198 + lysR BNILDI_14075 0.58 -2.0
2,837,198 + lysR BNILDI_14075 0.58 +0.2
2,837,480 - lysR BNILDI_14075 0.89 -0.9

Or see this region's nucleotide sequence